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OfflineClc420
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Are my jars taking too long to colenize?
    #26554391 - 03/24/20 03:59 AM (3 years, 10 months ago)

24 days ago my jars germinated and had myc growing. I shook last Wednesday and they recovered in just over a day and I have myc growing nicely spread out on the jars. But a I feel it should be quicker.. is 24 days too long and would you wait it out or start over ? Temps are 22C


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Offlineantmanmax
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Re: Are my jars taking too long to colenize? [Re: Clc420]
    #26554395 - 03/24/20 04:09 AM (3 years, 10 months ago)

24 days isn't insanely long from spores to 100% colonization, but it's on the longer side. If you shook and didn't see any contams take over you're probably good to go.


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OfflineClc420
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Re: Are my jars taking too long to colenize? [Re: antmanmax]
    #26554398 - 03/24/20 04:16 AM (3 years, 10 months ago)

Quote:

antmanmax said:
24 days isn't insanely long from spores to 100% colonization, but it's on the longer side. If you shook and didn't see any contams take over you're probably good to go.





Whats your average time on ms 100% colonization? No everything was clean, I had a bit of bruising after the shake but the myc has covered up bruising now. I really dont want to have to start over. Its NY 3rd time due to shit spore vendors but I had germination in 6 days from this. So you think I should be good to wait it out? I'm planning on cloning fruits when I grown them out too


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Offlineantmanmax
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Re: Are my jars taking too long to colenize? [Re: Clc420]
    #26554404 - 03/24/20 04:24 AM (3 years, 10 months ago)

Quote:

Clc420 said:
Quote:

antmanmax said:
24 days isn't insanely long from spores to 100% colonization, but it's on the longer side. If you shook and didn't see any contams take over you're probably good to go.





Whats your average time on ms 100% colonization? No everything was clean, I had a bit of bruising after the shake but the myc has covered up bruising now. I really dont want to have to start over. Its NY 3rd time due to shit spore vendors but I had germination in 6 days from this. So you think I should be good to wait it out? I'm planning on cloning fruits when I grown them out too




I never do spore to grains, so I couldn't tell you, but I've seen people have 100% colonization in two weeks, and 100% colonization in 6 weeks. IMO there's no sense learning sterile technique and then ruining it with a dirty spore print / syringe as you've experienced.

It looks good for now, you may very well get some fruits out of that, but my best advice to you is to learn agar. It will save you so much time in the long run.


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Edited by antmanmax (03/24/20 04:26 AM)


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OfflineSockadin
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Re: Are my jars taking too long to colenize? [Re: antmanmax]
    #26554405 - 03/24/20 04:25 AM (3 years, 10 months ago)

I don't usually get bruising from a shake
Got a picture?


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OfflineClc420
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Re: Are my jars taking too long to colenize? [Re: antmanmax]
    #26554407 - 03/24/20 04:28 AM (3 years, 10 months ago)

Quote:

Sockadin said:
I don't usually get bruising from a shake
Got a picture?




Quote:

antmanmax said:
Quote:

Clc420 said:
Quote:

antmanmax said:
24 days isn't insanely long from spores to 100% colonization, but it's on the longer side. If you shook and didn't see any contams take over you're probably good to go.





Whats your average time on ms 100% colonization? No everything was clean, I had a bit of bruising after the shake but the myc has covered up bruising now. I really dont want to have to start over. Its NY 3rd time due to shit spore vendors but I had germination in 6 days from this. So you think I should be good to wait it out? I'm planning on cloning fruits when I grown them out too




I never do spore to grains, so I couldn't tell you, but I've seen people have 100% colonization in two weeks, and 100% colonization in 6 weeks. IMO there's no sense learning sterile technique and then ruining it with a dirty spore print / syringe as you've experienced.

It looks good for now, you may very well get some fruits out of that, but my best advice to you is to learn agar. It will save you so much time in the long run.





I've got everything to go with agar now, my hepa is being delivered this week hopefully so I'll be on the agar track from now on. I only started from ms so I can get my own culture


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OfflineClc420
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Re: Are my jars taking too long to colenize? [Re: Sockadin]
    #26554409 - 03/24/20 04:29 AM (3 years, 10 months ago)

Quote:

Sockadin said:
I don't usually get bruising from a shake
Got a picture?




No? It was blue brusing that happened within an hour of the shake. It's gone/covered by new myc now


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OfflineClc420
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Re: Are my jars taking too long to colenize? [Re: antmanmax]
    #26554424 - 03/24/20 05:03 AM (3 years, 10 months ago)

Quote:

antmanmax said:
Quote:

Clc420 said:
Quote:

antmanmax said:
24 days isn't insanely long from spores to 100% colonization, but it's on the longer side. If you shook and didn't see any contams take over you're probably good to go.





Whats your average time on ms 100% colonization? No everything was clean, I had a bit of bruising after the shake but the myc has covered up bruising now. I really dont want to have to start over. Its NY 3rd time due to shit spore vendors but I had germination in 6 days from this. So you think I should be good to wait it out? I'm planning on cloning fruits when I grown them out too




I never do spore to grains, so I couldn't tell you, but I've seen people have 100% colonization in two weeks, and 100% colonization in 6 weeks. IMO there's no sense learning sterile technique and then ruining it with a dirty spore print / syringe as you've experienced.

It looks good for now, you may very well get some fruits out of that, but my best advice to you is to learn agar. It will save you so much time in the long run.





With spores on agar, how to you go about it? Cant you end up with sub strains that wont fruit ? Do you inoc a whole petri dish and inoc a hard with that whole plate ?


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OfflineBooShow
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Re: Are my jars taking too long to colenize? [Re: Sockadin]
    #26554425 - 03/24/20 05:05 AM (3 years, 10 months ago)

Quote:

Sockadin said:
I don't usually get bruising from a shake
Got a picture?



Really? I notice bruising in a lot of my jars after I shake 'em.


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You are what is. That's all.


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Offlinepoisoned
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Re: Are my jars taking too long to colenize? [Re: Clc420]
    #26554693 - 03/24/20 08:35 AM (3 years, 10 months ago)

I highly recommend you to read all of this:
https://www.shroomery.org/forums/showflat.php/Number/22721954

You'll see that you don't need a HEPA and that there's a shit ton of ways how to inoculate with it.


--------------------
How I do glass dishes


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OfflineBallzagna
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Re: Are my jars taking too long to colenize? [Re: poisoned] * 2
    #26554977 - 03/24/20 11:41 AM (3 years, 10 months ago)

Quote:

Clc420 said:
Quote:

antmanmax said:

I never do spore to grains, so I couldn't tell you, but I've seen people have 100% colonization in two weeks, and 100% colonization in 6 weeks. IMO there's no sense learning sterile technique and then ruining it with a dirty spore print / syringe as you've experienced.

It looks good for now, you may very well get some fruits out of that, but my best advice to you is to learn agar. It will save you so much time in the long run.





With spores on agar, how to you go about it? Cant you end up with sub strains that wont fruit ? Do you inoc a whole petri dish and inoc a hard with that whole plate ?





I've only recently started with agar(wish I'd have done it in the first place, highly recommend you do as well), but you can have multi spore agar plates in addition to isolating strains.

To my understanding, some isolates won't fruit, but at the same time multi spore is essentially a genetic crapshoot, but the odds of having fruiting genetics are extremely high but it'll never be consistent from grow to grow.

I swabbed 8 initial plates from a spore print. If you were to use a syringe, just use one drop per plate. It'll also stretch out the usability of your syringes too. I saw growth within 2-3 days but I waited a little longer to do a transfer.

I then took a transfer from each MS plate to put on 8 new plates. I kept the MS plates around and put 3 good looking ones to grain for a MS shoebox grow that I'm going to try to take a clone or two from/get started on an actual grow. I left the remaining MS plates alone to see if they'll pin to try to clone that as well but I have no idea what's going to happen  :smile2:

My goal is to have 3 MS shoeboxes, maybe get a clone or two if I can figure that out but I just want a successful grow under my belt.

While I do that, I'm going to take transfers from plates and trying until I isolate 10 strains to test in shoeboxes at once and decide which one to keep.

Pasty Plates

Stro's Isolating/Cleaning

Bod's Shoebox

If anything, this'll give you something to do while you wait around for it to finish colonizing  :awethumb:


--------------------
No one likes a naysayer

It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine.
PE Adventure
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OfflineClc420
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Re: Are my jars taking too long to colenize? [Re: Ballzagna]
    #26555320 - 03/24/20 03:28 PM (3 years, 10 months ago)

Quote:

Ballzagna said:
Quote:

Clc420 said:
Quote:

antmanmax said:

I never do spore to grains, so I couldn't tell you, but I've seen people have 100% colonization in two weeks, and 100% colonization in 6 weeks. IMO there's no sense learning sterile technique and then ruining it with a dirty spore print / syringe as you've experienced.

It looks good for now, you may very well get some fruits out of that, but my best advice to you is to learn agar. It will save you so much time in the long run.





With spores on agar, how to you go about it? Cant you end up with sub strains that wont fruit ? Do you inoc a whole petri dish and inoc a hard with that whole plate ?





I've only recently started with agar(wish I'd have done it in the first place, highly recommend you do as well), but you can have multi spore agar plates in addition to isolating strains.

To my understanding, some isolates won't fruit, but at the same time multi spore is essentially a genetic crapshoot, but the odds of having fruiting genetics are extremely high but it'll never be consistent from grow to grow.

I swabbed 8 initial plates from a spore print. If you were to use a syringe, just use one drop per plate. It'll also stretch out the usability of your syringes too. I saw growth within 2-3 days but I waited a little longer to do a transfer.

I then took a transfer from each MS plate to put on 8 new plates. I kept the MS plates around and put 3 good looking ones to grain for a MS shoebox grow that I'm going to try to take a clone or two from/get started on an actual grow. I left the remaining MS plates alone to see if they'll pin to try to clone that as well but I have no idea what's going to happen  :smile2:

My goal is to have 3 MS shoeboxes, maybe get a clone or two if I can figure that out but I just want a successful grow under my belt.

While I do that, I'm going to take transfers from plates and trying until I isolate 10 strains to test in shoeboxes at once and decide which one to keep.

Pasty Plates

Stro's Isolating/Cleaning

Bod's Shoebox

If anything, this'll give you something to do while you wait around for it to finish colonizing  :awethumb:





Yeah my worry with spores to agar is are you gonna end up with a fruiting potential. I came up with a plan today and I'm gonna inoculate agar plates with spore syringes and transfer. If I get sectors I'm gonna take multiple sectors and put them into a jar to make liquid culture and then grow that out to get fruits so I can get clones from them. I dont want to try and isolate from spores, I'd rather isolate from clones.


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Offlineohfunkya
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Re: Are my jars taking too long to colenize? [Re: Clc420]
    #26555333 - 03/24/20 03:34 PM (3 years, 10 months ago)

24 days seems a bit long. What size are your jars? and grains or BRF? Ive had some BRF take about a month to fully colonize! and depending on jar sizes anything could have a long turn around.


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Offlinepoisoned
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Re: Are my jars taking too long to colenize? [Re: ohfunkya]
    #26555450 - 03/24/20 04:34 PM (3 years, 10 months ago)

When you’re growing on agar, you won’t just isolate a culture randomly. There’s practically zero worry  that you’re going to get anything that doesn’t fruit in a few transfers.


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OfflineClc420
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Re: Are my jars taking too long to colenize? [Re: poisoned]
    #26556369 - 03/25/20 05:17 AM (3 years, 9 months ago)

Quote:

poisoned said:
When you’re growing on agar, you won’t just isolate a culture randomly. There’s practically zero worry  that you’re going to get anything that doesn’t fruit in a few transfers.





Yeah I know that but say after 2 transfers can you still take a small peice if mycelium and still get multiple sub strains from that ?


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InvisibleInfiniteDreams
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Re: Are my jars taking too long to colenize? [Re: Clc420] * 1
    #26556392 - 03/25/20 06:07 AM (3 years, 9 months ago)

Yes.  Your plan to grow some fruits and clone from there is more efficient.


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Offlinepoisoned
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Re: Are my jars taking too long to colenize? [Re: Clc420]
    #26556409 - 03/25/20 06:28 AM (3 years, 9 months ago)

I'd say chances of having something that won't fruit after 5 transfers are magnitudes lower than having a contamination when going MS->grain.


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