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OfflineZombie-ant
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Registered: 09/02/19
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Last seen: 16 days, 12 hours
Petri Pin
    #26554303 - 03/24/20 01:30 AM (3 years, 10 months ago)

I do wrong
I do right
Sing my songs
Even in plight
Mycelial growth
Mycelium fights
In the Petri
A pin’s in sight

What should I do
What should I give
To make sure
This baby does live
Do I do
What I’ve just done
what is hard
But can be fun
Even though
it is a pain
Do I  do transfers
Again and again?



Edited by Zombie-ant (03/24/20 03:01 AM)


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Offlinejbgtaa
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Re: Petri Pin [Re: Zombie-ant]
    #26554312 - 03/24/20 01:58 AM (3 years, 10 months ago)

Let it grow for a couple more days and clone it. Strong fruitier, those petri pins are.


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OfflineSockadin
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Re: Petri Pin [Re: jbgtaa]
    #26554414 - 03/24/20 04:38 AM (3 years, 10 months ago)

What is with all the discoloration on the dish? Just digested agar?


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OfflineBooShow
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Re: Petri Pin [Re: Sockadin] * 1
    #26554415 - 03/24/20 04:40 AM (3 years, 10 months ago)

It looks so lonely :frown:


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You are what is. That's all.


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OfflineZombie-ant
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Re: Petri Pin [Re: Sockadin]
    #26554453 - 03/24/20 05:36 AM (3 years, 10 months ago)

Quote:

Sockadin said:
What is with all the discoloration on the dish? Just digested agar?



My poor attempt at making BRF agar


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OfflineZombie-ant
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Re: Petri Pin [Re: Zombie-ant] * 1
    #26554467 - 03/24/20 05:45 AM (3 years, 10 months ago)

Quote:

BooShow said:
It looks so lonely :frown:



🤣

Aptly sized violin needed 🎻



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InvisibleSunny Skies
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Re: Petri Pin [Re: Sockadin] * 1
    #26554497 - 03/24/20 06:25 AM (3 years, 10 months ago)

Fingers I think


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OfflineZombie-ant
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Re: Petri Pin [Re: Sunny Skies]
    #26554512 - 03/24/20 06:39 AM (3 years, 10 months ago)

Quote:

Sunny Skies said:
Fingers I think



?


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OfflineBooShow
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Re: Petri Pin [Re: Zombie-ant]
    #26554521 - 03/24/20 06:47 AM (3 years, 10 months ago)

Sunny Skies got his head in da clouds :aweohyou:


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OfflineZombie-ant
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Re: Petri Pin [Re: jbgtaa]
    #26557480 - 03/25/20 05:17 PM (3 years, 9 months ago)

Quote:

jbgtaa said:
Let it grow for a couple more days and clone it. Strong fruitier, those petri pins are.




How comes it make the fruit stronger?


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InvisibleLadysKnight
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Re: Petri Pin [Re: Zombie-ant]
    #26557524 - 03/25/20 05:53 PM (3 years, 9 months ago)

Cuz it's able to fruit despite imperfect conditions, like a petri dish.


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OfflineZombie-ant
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Re: Petri Pin [Re: LadysKnight]
    #26557556 - 03/25/20 06:06 PM (3 years, 9 months ago)

Quote:

LadysKnight said:
Cuz it's able to fruit despite imperfect conditions, like a petri dish.



Fair enough.

Would it be best to slice it it half lengthwise, and then slice the halves up and transfer them to separate dishes?


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Offlinepoisoned
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Re: Petri Pin [Re: Zombie-ant]
    #26557564 - 03/25/20 06:09 PM (3 years, 9 months ago)

Probably better to clone the whole pin and do transfers later.


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OfflineSockadin
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Re: Petri Pin [Re: Zombie-ant]
    #26557651 - 03/25/20 07:00 PM (3 years, 9 months ago)

Quote:

Zombie-ant said:
Quote:

LadysKnight said:
Cuz it's able to fruit despite imperfect conditions, like a petri dish.



Fair enough.

Would it be best to slice it it half lengthwise, and then slice the halves up and transfer them to separate dishes?





No is the simplest answer. The more you touch it the more you increase the contamination rate. Flames some tweezers pluck and out in a new dish.


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OfflineZombie-ant
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Re: Petri Pin [Re: Sockadin]
    #26558687 - 03/26/20 09:23 AM (3 years, 9 months ago)

Quote:

Sockadin said:
Quote:

Zombie-ant said:
Quote:

LadysKnight said:
Cuz it's able to fruit despite imperfect conditions, like a petri dish.



Fair enough.

Would it be best to slice it it half lengthwise, and then slice the halves up and transfer them to separate dishes?





No is the simplest answer. The more you touch it the more you increase the contamination rate. Flames some tweezers pluck and out in a new dish.




cool, should I pluck or cut its gotten bigger



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OfflineSockadin
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Re: Petri Pin [Re: Zombie-ant]
    #26558696 - 03/26/20 09:26 AM (3 years, 9 months ago)

It will come right off the agar with ease and some tweezers.


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OfflineZombie-ant
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Re: Petri Pin [Re: Sockadin]
    #26563438 - 03/28/20 03:04 PM (3 years, 9 months ago)

I Have Two more Petri dishes pinning now




Also I got to the other one too late with all the covid-19 happening and my kids needing attention.
I cut it in half and noticed a few minutes ago that the cap half (last pic) has made a spore print on agar.
Is that an issue?


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OfflineSockadin
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Re: Petri Pin [Re: Zombie-ant]
    #26563587 - 03/28/20 04:09 PM (3 years, 9 months ago)

Yes because now instead of a clone you will grow a MS culture.


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OfflineZombie-ant
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Re: Petri Pin [Re: Sockadin]
    #26563738 - 03/28/20 05:15 PM (3 years, 9 months ago)

Quote:

Sockadin said:
Yes because now instead of a clone you will grow a MS culture.



duh,

Is it advisable to cut the bottom part and transfer?


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Invisiblewoofwoof
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Registered: 01/04/19
Posts: 1,127
Re: Petri Pin [Re: Zombie-ant]
    #26563789 - 03/28/20 05:32 PM (3 years, 9 months ago)

just take flame sterilized tweezers and pick it out of the agar in whole piece then move to a clean blank agar dish


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