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OfflineMycocoirs
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Germinating Spores on Agar, Cleaning Away From Mold Contamination Help Plz
    #26554257 - 03/24/20 12:34 AM (3 years, 10 months ago)

Hey guys, so i had a verticillium contamination and had to throw out most of my spores that i had saved. 

Recently I purchased some new spores from spore supplier and put them to agar.  All three spore prints (from two different vendor batches) have a mold contamination that germinates along with the mushroom spores. 

I have tried multiple ways of inoculating the spores to agar. Including a spore swipe to dilute the colonies and hopefully get one that dosent have mold germinating with the mycelium.

Usually first transfer away from spore plates will result in rhizomorphic mycelium growing in that fashion because it has this mold growing over top of it,


I have tried to sample small pieces of mycelium from the leading edge of the rhizo growth but the second transfer plate seems to grow slowly and contain the mold contamination in it still..


Any advice how to get away from mold that is growing over top of your mycelium? Should i try getting new spores from a new vendor?

Is there a way i could be inoculating and transferring off agar differently?

Or are these spores far to contaminated with said mold in order to be able to germinate and isolate clean mycelium (remember 3 diff prints from 2 batches of GT from spore vendor)


Edited by Mycocoirs (03/24/20 11:19 AM)


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OfflineMycocoirs
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Re: Germinating Spores on Agar, Cleaning Away From Mold Contamination Help Plz [Re: Mycocoirs]
    #26554282 - 03/24/20 01:06 AM (3 years, 10 months ago)

Such a pain because it looks clean on first transfer.  The mycelium is appearing rhizomorphic on first transfer like this because of the mold its trying to escape?


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Invisibletramalot
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Re: Germinating Spores on Agar, Cleaning Away From Mold Contamination Help Plz [Re: Mycocoirs]
    #26554421 - 03/24/20 04:51 AM (3 years, 10 months ago)

You can get antibiotic to add to your agar. You can get it $25gr from a site supporter.

I don't think your supposed to post vendor names.


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OfflineMycocoirs
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Re: Germinating Spores on Agar, Cleaning Away From Mold Contamination Help Plz [Re: tramalot]
    #26554932 - 03/24/20 11:20 AM (3 years, 10 months ago)

ok thanks for that i edited the post,

and antibiotic will not help as this is a fungal (mold) contamination, not bacterial.


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