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InvisibleDeckardCain
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Opinions on these plates and lc questions
    #26552973 - 03/23/20 11:58 AM (3 years, 10 months ago)

How do these plates look, would one be preferable if so why? And would you put one of these to lc?
I recently did agar to grain and a lot of them failed all my other jars have been grain to grain transfers from an orginal syringe and they have went a lot better, still could tell there were contams as the fruits got a lot shorter. I probably have bad technique for transferring agar to grain, I tried cutting a bunch of squares on one attempt and just plucking them all up and tossing them in the jar, and I’ve tried cutting 4 or 5 wedges and putting one to each jar, both have had some failures, 2 blueish Mold and most the rest have this nasty pink Mold that spreads to every single grain after a shake. My guess is they get on the agar after they’re cut and on the way into the jar as the lid is on almost the whole time



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Offlinerumfor69
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Re: Opinions on these plates and lc questions [Re: DeckardCain]
    #26553065 - 03/23/20 12:42 PM (3 years, 10 months ago)

Those look good. Are you using a flowhood or SAB?

I'd suggest keep making transfers and keep tossing
wedges in grains trying to cultivate a healthy master
jar.

What grains are you using and what tek?

Are you going to go with a monotub design?


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InvisibleDeckardCain
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Re: Opinions on these plates and lc questions [Re: rumfor69]
    #26553269 - 03/23/20 02:58 PM (3 years, 10 months ago)

I have 2 jars that look strong to me right now I’m thinking of using as masters, I’m using oats (BOD’s oat tek) probably use a mono tub , and I use a still air box, a flow hood would be nice for my next investment.

I inoculated 4 or 5 oyster jars on the same batch of oats and the oyster myc seemed to grow so fast I didn’t get any contams at all, maybe just luck. If you were to pick one tho for an lc which would you use and how big should the wedge be?

Don’t ask why the pictures are sideways I have no idea
These are contaminated thinking about top fruiting


These are what I hope to be my masters


These are very questionable, shook recently but they’re looking really weak
]

These I’m not yet sure of, super slow growth I think I over dried the grain on this run


Edited by DeckardCain (03/23/20 03:06 PM)


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Offlinerumfor69
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Re: Opinions on these plates and lc questions [Re: DeckardCain]
    #26553387 - 03/23/20 04:04 PM (3 years, 10 months ago)

I'm not a fan of LC personally cause you can't tell if it's contaminated.

It's better to just toss agar wedges in grains making master jars
and using those jars in grain to grain transfer to other jars.

The SAB will work but will lower your percentage success rate imo
so just always make more of everything than you need so you have
extra chances at it.

Also your lid setup needs upgraded. You gotta get synthetic filter disks
and do the 4 1/8" holes in lids ring set up. It really is awesome,cheap, and
You can even buy aluminum rings and lids off Amazon so they never rust.


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Edited by rumfor69 (03/23/20 04:06 PM)


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Offlineshevanel
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Re: Opinions on these plates and lc questions [Re: rumfor69]
    #26553405 - 03/23/20 04:13 PM (3 years, 10 months ago)

Myc on oats looks wierd compared to other grains.

Are you pc those oat at 2 or 3 hours?

Anything less than 3 and oats fail for me.

Ive since gone back to millet and ill never change. Just so much more consistent and easier to get properly hydrated.


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OfflineTankie_J
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Re: Opinions on these plates and lc questions [Re: rumfor69]
    #26553447 - 03/23/20 04:31 PM (3 years, 10 months ago)

Quote:

rumfor69 said:
Also your lid setup needs upgraded. You gotta get synthetic filter disks
and do the 4 1/8" holes in lids ring set up. It really is awesome,cheap, and
You can even buy aluminum rings and lids off Amazon so they never rust.




I agree. And I also agree that your plates look good. I think it’s probably your jar setup.

I do all my work in a SAB and have a very low contam rate. Shit, I even did some A2G and didn’t realize I was out of gloves. All the jars (9) fully colonized no contam. And if your plate transfers are coming out like that, it seems as though you got it.

I personally only take a max of two large transfers from any given plate, one at a time, for grain. The less time those lids are off the better. First I take the ring off the receiving jar, make sure the lid will come off easy. Then I make the transfer from the agar plate. As quick, but with slow steady movements, as possible.

Keep it up, you’ll get there


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InvisibleDeckardCain
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Re: Opinions on these plates and lc questions [Re: Tankie_J]
    #26553500 - 03/23/20 04:52 PM (3 years, 10 months ago)

half my jars use synthetic filter disks half use poly fill I see no difference in which get contams, I’ve read bod post poly works fine for him, and also before using a2g I had no noticeable moulds just some sorts of bacteria that got in over the course of 3-4 g2g generations. So I think my lids really aren’t my problem so much as my sterile technique. I should probably do fewer wedges like suggested as well next time

About to pick these now, as the generations of g2gs have gone on they get shorter


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Edited by DeckardCain (03/23/20 04:56 PM)


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Offlinerumfor69
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Re: Opinions on these plates and lc questions [Re: DeckardCain]
    #26553621 - 03/23/20 05:58 PM (3 years, 10 months ago)

Multiple generations of g2g causes the mycelium to genetically breakdown
over the distance it's having to grow. G2G doesn't make new mycelium
but is just an extension of the mycelium network. Master jar - g2g to more jars - spawn to sub.
Any further g2g transfers beyond that and it starts to breakdown.


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OfflineMachiavelliavore
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Re: Opinions on these plates and lc questions [Re: rumfor69]
    #26553706 - 03/23/20 06:37 PM (3 years, 10 months ago)

Your cultures look super clean.  Whatever's going on is most likely about the transfer technique or the lids.  I don't use band lids cause I find they come off in two pieces quite often, or if they leave just the top it's awkward to get off.  Anyway, just make sure your hands are never over your jars, and you have a system for where everything goes.  Good luck, you'll get there.  A2G is my favorite inoculation method, though LI is quite good as well, just more work for greater speed.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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