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YukonXCornelius
Toadstool Enthusiast


Registered: 07/17/19
Posts: 22
Last seen: 2 years, 6 months
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Quick Question Vís-a-Vís: LC Contams
#26550350 - 03/22/20 07:42 AM (3 years, 10 months ago) |
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TL;DR: LC is clear but becomes cloudy after stirring it. Is this normal or does this indicate contams? (Pics below)
Hello friends,
Yukon Cornelius here, (you can call me Yukon ): first time poster, long time lurker.
Lemme start by thanking you fine gentlemen, ladies, gender non-conformists, etc. for all the help you have unknowingly provided me over the past year and change.
I had a [hopefully] quick question that I’m certain one of your OG cultivators could answer *fingers crossed*
I’ve taken a shot at making LC’s (for shits and giggles) after working with agar for a hot minute. In a nutshell:
I have taken a syringe (boiled for 30 min beforehand) and filled it with sterile water. I then (under the protection of my SAB) squirted said water (through a DIY silicone ship) into a “dish” (ie: 1/2 pint mason jar) which had a vigorously pan specimen growing on it (which was sliced up using a flame sterilized scalpel moments prior to the addition of the water). The now myc rich water “slurry” was sucked into the syringe and ejected into a freshly sterilized LME liquid culture (made ~125ml H2O + 0.25g dry, light malt extract). This was about 48 hours ago.
I’ve been looking at the LC’s for which this procedure was performed and had a burning question based on what I’m witnessing:
It seems to be the consensus that cloudy = no bueno  My LC’s appear to be as clear as they were the moment they were removed from my PC, post-sterilization, EXCEPT after being agitated/stirred by yours truly.
With the exception of maybe 1 out of 4, all of these LC’s contained no visible sediment prior to inoculation and all were strained prior to sterilization.
So, now that you have all the background info that (I would imagine) is necessary, can any of you fine folks chime in and let me know what you think with regard to the possibility of my LC’s being contaminated (or at the very least, not being up to snuff, visually speaking anyway)
Here are the pics I promised:
Before agitating/stirring:


(Close up of mycelium pre-agitation)

After agitation/stirring:


Thanks in advance! Y’all are doin the lord’s work!
-------------------- -Yukon X. Cornelius “BUMBLES BOUNCE!” https://imgur.com/gallery/81KphGM
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bw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,937
Loc: 7b
Last seen: 23 hours, 6 minutes
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we cannot tell you if your LC is contaminated. Use it and find out.
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YukonXCornelius
Toadstool Enthusiast


Registered: 07/17/19
Posts: 22
Last seen: 2 years, 6 months
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Re: Quick Question Vís-a-Vís: LC Contams [Re: bw86]
#26551706 - 03/22/20 08:14 PM (3 years, 10 months ago) |
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Quote:
bw86 said: we cannot tell you if your LC is contaminated. Use it and find out.
Ok well you either (A) didn’t read my post beyond the title (...not even the “TL;DR” section I wrote at the top of the post specifically for members who won’t/can’t read posts beyond a paragraph, give or take)...
Or (B) you DID, in fact, read it, so the sub-par, elitist response I got can be chalked up to laziness or perhaps being intimidated as a result of knowing fuck-all about LC’s. Both options bad, and as a long time member and apparent spokesperson for Shroomery (“we cannot tell you...”) one would expect a response that’s much less off-putting instead of playing into the holier-than-thou behavior that this site has become known for across varying mushroom based communities (and it’s ironic because it wasn’t long ago I was playing devils advocate and saying how Shroomery has probably changed since the time of elitism and alienation... those words taste about as good as the humble pie I’ll be having when the members of those communities tell me “I told ya so!”)
Anywho, if any other members who know about LC’s want to ignore all this bullshit and help me with my original inquiry, I would be very grateful for your expertise. It would also serve a second purpose of helping to welcome a n00b and show them that Shroomery is a welcoming place chock full o’ nice people willing to answer legitimate questions instead of lazy ones who condescend to new members they don’t know.
Thanks in advance...
-------------------- -Yukon X. Cornelius “BUMBLES BOUNCE!” https://imgur.com/gallery/81KphGM
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 8 minutes, 55 seconds
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I’m not super experienced with lc but mine were crystal clear. I had some go cloudy and they smelled horrible when opened.
I used water and a tiny bit of lme.
Here’s an old pic where I was asking the same thing.
One on the left was bad

That’s an extreme case, I’ve since had some go bad but just slightly cloudy.
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LAGM2020     
Edited by A.k.a (03/22/20 09:28 PM)
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