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OfflineEysea
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Registered: 02/25/20
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Grow log and Questions
    #26549927 - 03/21/20 10:41 PM (3 years, 10 months ago)

Agar is hard man been a month full of contams but just 2 weeks ago I received a wild print from a friend, I inoc 5 plates from it on the 13th of march and did T1 on the 16th. these are the last 2 plates from that batch and I just wanna know if they are clean and would be enough to start grains with them. If not where could I do sectoring? or should I still transfer and wait till a rhizomorphic myc forms? I don't know what Im supposed to do next.

Plate no.1 T1


Plate no.2 T1


Thanks for the answers and guides


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OfflineMorbility
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Re: Grow log and Questions [Re: Eysea]
    #26549970 - 03/21/20 11:18 PM (3 years, 10 months ago)

I'd do 1 or 2 more transfers at least before considering grain. Plate 1 looks pretty good. The 8 or 9 o'clock area seems more rhizo and where I'd transfer from. Plate 2 looks kinda funny, not necessarily contaminated though. I'd try grabbing a small wedge from between 5 and 6 o'clock to clean it up more.

You'll need more growth on the plates than what you have now before going to grain, but you're doing good so far!


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Offlinehazyhorse
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Re: Grow log and Questions [Re: Eysea]
    #26549972 - 03/21/20 11:21 PM (3 years, 10 months ago)

it might be clean but it's typically best practice to do at least 3 transfers to ensure there's no contamination. i've used t2 plates that looked clean & had luck. if you take a transfer take it anywhere from 6 to 9 on both pics


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InvisibleTranscendingLife
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Re: Grow log and Questions [Re: hazyhorse]
    #26549985 - 03/21/20 11:36 PM (3 years, 10 months ago)

4 oโ€™clock on both.
5 oโ€™clock on number 2 as well.

Look at the aggressive growth of both.
6.5 on number 2 I would not go close to, that extra dot worries me.

As previously stated, neither are ready for grain.


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OfflineEysea
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Re: Grow log and Questions [Re: TranscendingLife]
    #26550017 - 03/22/20 12:12 AM (3 years, 10 months ago)

Thank you so much for the inputs

but is it safe to let this grow out for a day or 2 before doing a transfer or should I do it asap?


Edited by Eysea (03/22/20 12:16 AM)


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OfflineMorbility
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Re: Grow log and Questions [Re: Eysea]
    #26550501 - 03/22/20 09:07 AM (3 years, 10 months ago)

The earliest you can make a transfer is always better since it gives any possible contaminates less time to establish on the plate. That being said, I don't think waiting a day or 2 is going to hurt anything.


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