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Eysea
Neophyte in myco



Registered: 02/25/20
Posts: 9
Loc: philippines
Last seen: 3 years, 7 months
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Grow log and Questions
#26549927 - 03/21/20 10:41 PM (3 years, 10 months ago) |
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Agar is hard man been a month full of contams but just 2 weeks ago I received a wild print from a friend, I inoc 5 plates from it on the 13th of march and did T1 on the 16th. these are the last 2 plates from that batch and I just wanna know if they are clean and would be enough to start grains with them. If not where could I do sectoring? or should I still transfer and wait till a rhizomorphic myc forms? I don't know what Im supposed to do next.
Plate no.1 T1

Plate no.2 T1

Thanks for the answers and guides
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Morbility
Cybernetic Psychonaut


Registered: 12/04/18
Posts: 166
Loc: Ilus IV
Last seen: 1 year, 7 months
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Re: Grow log and Questions [Re: Eysea]
#26549970 - 03/21/20 11:18 PM (3 years, 10 months ago) |
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I'd do 1 or 2 more transfers at least before considering grain. Plate 1 looks pretty good. The 8 or 9 o'clock area seems more rhizo and where I'd transfer from. Plate 2 looks kinda funny, not necessarily contaminated though. I'd try grabbing a small wedge from between 5 and 6 o'clock to clean it up more.
You'll need more growth on the plates than what you have now before going to grain, but you're doing good so far!
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,821
Last seen: 8 days, 11 hours
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Re: Grow log and Questions [Re: Eysea]
#26549972 - 03/21/20 11:21 PM (3 years, 10 months ago) |
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it might be clean but it's typically best practice to do at least 3 transfers to ensure there's no contamination. i've used t2 plates that looked clean & had luck. if you take a transfer take it anywhere from 6 to 9 on both pics
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Grow log and Questions [Re: hazyhorse]
#26549985 - 03/21/20 11:36 PM (3 years, 10 months ago) |
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4 oโclock on both. 5 oโclock on number 2 as well.
Look at the aggressive growth of both. 6.5 on number 2 I would not go close to, that extra dot worries me.
As previously stated, neither are ready for grain.
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Eysea
Neophyte in myco



Registered: 02/25/20
Posts: 9
Loc: philippines
Last seen: 3 years, 7 months
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Thank you so much for the inputs
but is it safe to let this grow out for a day or 2 before doing a transfer or should I do it asap?
Edited by Eysea (03/22/20 12:16 AM)
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Morbility
Cybernetic Psychonaut


Registered: 12/04/18
Posts: 166
Loc: Ilus IV
Last seen: 1 year, 7 months
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Re: Grow log and Questions [Re: Eysea]
#26550501 - 03/22/20 09:07 AM (3 years, 10 months ago) |
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The earliest you can make a transfer is always better since it gives any possible contaminates less time to establish on the plate. That being said, I don't think waiting a day or 2 is going to hurt anything.
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