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Invisiblethe_chosen_one
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos] * 1
    #26582858 - 04/06/20 09:51 PM (3 years, 9 months ago)

:highfive:
Someone spray some agar plates next. :laugh2:. If it turns out we've been wasting our time with PC'S and flow hoods then maybe this time I have seen it all :facepalm:
Pretty bad ass.
I'm really not that surprised though.  Cubensis are tough and aggressive like oysters.  Oysters can process all kinds of wicked chemicals.. so why not? Mushrooms are cool. :shrug:
I am curious about the longevity of the spores in the solution.  Really need to fix that time machine lol. Guess it wouldn't be critical if this repeats easily.  Ooooh rabbit holes!
I really want to spray an agar plate now :lol:


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"Luck favors the observant." - Workman



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Invisiblewoofwoof
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: the_chosen_one]
    #26590563 - 04/10/20 09:04 AM (3 years, 9 months ago)

Any updates?

Have you tried several to confirm that it works?


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OfflineLogicaL ChaosM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: woofwoof]
    #26590576 - 04/10/20 09:13 AM (3 years, 9 months ago)

I'll post an update today. I got 5 PF jars all showing white mycelium. Pretty cool :awesome:

The_chosen_one, im curious if cubenis spores could take a direct hit of Lysol and still germinate. Feel free to experiment :awehigh:


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OfflineLogicaL ChaosM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos]
    #26592331 - 04/10/20 10:34 PM (3 years, 9 months ago)

Photos

My previous batch with no treatment, open air spore print syringe:



My current batch of 5 PF jars with the open-air spore print with Lysol spray spritz Spore Syringe treatment added:


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InvisibleDarkslide
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos]
    #26633891 - 04/28/20 09:07 AM (3 years, 8 months ago)

Quote:

LogicaL Chaos said:
Photos

My previous batch with no treatment, open air spore print syringe:







Yep. That looks like Spring in the PNW alright. :lol:


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InvisiblefahtsterM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: Darkslide] * 1
    #26640194 - 05/01/20 12:10 AM (3 years, 8 months ago)

How these cakes doing?

Faht


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OfflineLogicaL ChaosM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: fahtster]
    #26640198 - 05/01/20 12:14 AM (3 years, 8 months ago)

Funny u say that, i was going to take some pics this weekend. They are doing excellent! They are almost ready for birthing. Im really surprised this worked. Its kinda scary how well it worked considering i did open-air printing in a room that is dusty and has air-leaks to the outside.

Im curious to see what kind of fruits come out of it. Perhaps some artifical selection has occurred due to the presence of the Lysol....

Im excited to try anti-bacterial soap next. I think it will work but cant be sure.


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InvisiblefahtsterM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos] * 1
    #26640215 - 05/01/20 12:23 AM (3 years, 8 months ago)

Yeah.. that’s super cool it worked (knock on wood). You think there was just so many cube spores that they survived due to shear numbers compared to the probably small amount of contamination? 

Faht


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OfflineLogicaL ChaosM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: fahtster]
    #26640257 - 05/01/20 12:47 AM (3 years, 8 months ago)

I believe so. I used extra spores because it was my belief that most would not survive the harshness of the Lysol. And in doing so, maybe there was some "survival of the fittest" action going on.

Wish i had a microscope so i can see whats rrally happening. Might have to pop into the Microscopy subforum for help.


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OfflineLogicaL ChaosM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos]
    #26642149 - 05/01/20 08:28 PM (3 years, 8 months ago)

All white jars. Some are even pinning. No infections. Only needed a small amount of Lysol in the syringe. Didnt think it would be this easy....



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InvisiblefahtsterM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos] * 1
    #26642280 - 05/01/20 09:52 PM (3 years, 8 months ago)

Does the Lysol say kill 99.99 percent of germs?  Looks like the .01% was cubie spores lol. Pretty rad

Faht


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OfflineLogicaL ChaosM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: fahtster]
    #26642368 - 05/01/20 11:00 PM (3 years, 8 months ago)

Yup, it says "99.9% of germs" on the back! Cube spores are special. That's what I learned.


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Offlinethe man
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos] * 1
    #26642490 - 05/02/20 12:26 AM (3 years, 8 months ago)

peroxide is neat too.... just dont want to eat most of that type of antibac stuff crazier more effective stuff esp.


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OfflineLogicaL ChaosM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: the man]
    #26642502 - 05/02/20 12:35 AM (3 years, 8 months ago)

No doubt. Im hestitiant to even eat the shrooms honestly. Maybe they are too clinical. Back the day, dealer shrooms felt "clinical", no love them, like a clinical drug. It was weird.


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InvisiblecoAsTal
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos] * 1
    #26643109 - 05/02/20 08:10 AM (3 years, 8 months ago)

FWIW, Hydrogen Peroxide breaks down relatively quickly into water and oxygen, so could conceptually be a good flash-sterilizer in that respect-- and if I'm not mistaken, bleach breaks down into water and salt pretty quickly- especially up around 80 degrees.

I know I've seen people talk about them not being the best solution before elsewhere though-- but I don't remember why


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Invisiblenooneman
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos] * 1
    #26643603 - 05/02/20 12:32 PM (3 years, 8 months ago)

I'm impressed that this worked at all. I didn't expect it to. When I first saw the thread title, honestly my initial reaction was "lol noobs that will never work" but then I saw it was posted by LC, and I was like "woah, LC posted this? Well, I guess I'll check it out."

And low and behold, those are some healthy looking jars! I guess I was the uninformed noob this time. Super impressed.

I'm surprised Alan hasn't shown up with some spores under microscope to show what the effect of antibacterial soap on spores is. Maybe someone can get him in this thread later on for some science. I have some doubts about hydrogen peroxide given its effects on mycelium, but it seems like some good old fashioned antibacterial soap might have some use!

Cube spores are amazingly sturdy and resilient.


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OfflineLogicaL ChaosM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: nooneman]
    #26643610 - 05/02/20 12:35 PM (3 years, 8 months ago)

I didn't think it would work either. I know I was spore-printing dirty. The reason I know was because I had so many shrooms growing last year that I got lazy with printing and just started mass-printing open-air which is the worst way to do it. So I had like 30 spore prints but they were all dirty. Pretty dumb in retrospect...

I should PM Alan. I bet he might pick this up....

I heard mushroom spores can survive in space! Not sure if its true, but if it is, could explain this Lysol situation.


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Offlinesporecap
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos] * 2
    #26643793 - 05/02/20 01:50 PM (3 years, 8 months ago)

But if it doesn't kill the cube spores, what is it doing to all the contaminant spores like green mold?
Maybe it kills a huge fraction of everything, and then the mold spores are outnumbered by the cube spores?


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OfflineLogicaL ChaosM
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: sporecap]
    #26643796 - 05/02/20 01:51 PM (3 years, 8 months ago)

Im not really sure whats going at the microscopic level so right now its a bit of a mystery :shrug:

All I know is the mold spores that were on that spore print did not germinate while the cubenis spores did germinate.


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Invisiblethe_chosen_one
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Re: Add Anti-Bacterial Soap to a Syringe? [Re: LogicaL Chaos] * 1
    #26643859 - 05/02/20 02:21 PM (3 years, 8 months ago)

Mold spores and bacteria are quite a bit smaller. Suffracants typically soften cell walls in a lot of applications. Cubie spores appear tough and large enough to take it to an extent.

Can't get Lysol to save my life right now :lol: but a discussion about straw contamination led to Dawn dish soap. Any kidney killing chemical that saves not only ducks, but straw logs has gotta be worth a shot.  :shrug:


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