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InvisibleD3_Myc
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: eatyualive]
    #26604125 - 04/15/20 09:02 PM (3 years, 9 months ago)

Quote:

eatyualive said:
What are all the little circles in your plates? Looks like it’s a hole punch.




Yea I use a hollow leather punch for most my xfers unless I’m trying to grab something really small. I have OCD and the perfect circles are somewhat satisfying especially if the growth matches.


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OfflineA.k.aM
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: D3_Myc]
    #26604249 - 04/15/20 09:44 PM (3 years, 9 months ago)

Damn that yeti plate is nice.

Did your other rw plates have that type of growth?

Idk if it’s a coincidence but I tend to see myc exactly like that on my blue plates also.

Like this one looked totally different on the plate it came from



Starting to think it’s the coloring.


lol demonic you need to add an explanation about your hole punch to your signature


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LAGM2020


Edited by A.k.a (04/15/20 09:45 PM)


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InvisibleD3_Myc
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: A.k.a]
    #26604267 - 04/15/20 09:54 PM (3 years, 9 months ago)

Lol I’ll do a video one day once my technique is a little better. I still have bad habits of hovering over plates and stuff.

All my RW plates where tomentos,



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Invisiblewoofwoof
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: D3_Myc]
    #26604650 - 04/16/20 02:08 AM (3 years, 9 months ago)

Thanks eats!



I did 4 agar xfers and its still tomatose. Damn 6 xfers huh?


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Invisibleeatyualive
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: D3_Myc]
    #26604879 - 04/16/20 05:26 AM (3 years, 9 months ago)

That is really cool demonic. OCD or not. You should write a tek on it. I’m digging it. Do you have a link to the tool.

Something like this? Honestly that is pure genius man.

https://www.amazon.com/Spurtar-Carbon-Leather-Gaskets-Storage/dp/B07L1FBTZV/ref=mp_s_a_1_9?dchild=1&keywords=leather+hole+punch&qid=1587036649&sr=8-9

I’m interested which size you use and how do you pop the transfer out? The growth on your plates is near perfect. I noticed it on all the plates you posted and i was like there is no way there are perfect condensation circles on all of his plates. 😂

Yes al transfers I’ve done til this plate have been tomentose. T6


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Edited by eatyualive (04/16/20 05:43 AM)


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InvisibleD3_Myc
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: eatyualive]
    #26605630 - 04/16/20 11:17 AM (3 years, 9 months ago)

Quote:

eatyualive said:
That is really cool demonic. OCD or not. You should write a tek on it. I’m digging it. Do you have a link to the tool.

Something like this? Honestly that is pure genius man.

https://www.amazon.com/Spurtar-Carbon-Leather-Gaskets-Storage/dp/B07L1FBTZV/ref=mp_s_a_1_9?dchild=1&keywords=leather+hole+punch&qid=1587036649&sr=8-9

I’m interested which size you use and how do you pop the transfer out? The growth on your plates is near perfect. I noticed it on all the plates you posted and i was like there is no way there are perfect condensation circles on all of his plates. 😂

Yes al transfers I’ve done til this plate have been tomentose. T6





Eat, I get this proxy error when I click your link.



But these are the ones I ordered , only $7 right now. SS would probably be ideal if you can find it

OWDEN 9 PIECES HOLLOW PUNCH SET... https://www.amazon.com/dp/B07GCWY3HH?ref=ppx_pop_mob_ap_share

I use the 1/4” or 5/16” ones. They come with a little machine oil on them but a good iso wipe gets it off. Unless you have sterilizing bags I don’t recommend PC them as I tried one and it got some surface rust from water getting in the foil.

These don’t make the transfer, more like a cookie cutter that cuts perfect circles then scoop them up with your scalpel by stabbing through the side. Real quick and easy. Maybe I’ll write up a tek like you suggested in a few days. Also cover my really clear agar since a lot of people comment on how I get it that way.


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Invisibleeatyualive
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: D3_Myc]
    #26605973 - 04/16/20 02:20 PM (3 years, 9 months ago)

So you sterilize with a bernzo torch and then poke and lift and use scalpel to transfer?

Yes it is very clear.


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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: eatyualive]
    #26605980 - 04/16/20 02:25 PM (3 years, 9 months ago)

That's alot of work in and out of the dish, I wonder how we can make a tool that will do this, but do it all at once? Like a metal straw with your finger on the end to create a suction?

Demonic I love the cleanliness of this culture tool. It is very creative, but I get scared going in with multiple tools.


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InvisibleD3_Myc
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: Sockadin]
    #26606086 - 04/16/20 03:37 PM (3 years, 9 months ago)

Meh, it’s not so bad. It’s honestly faster than using just a scalpel. Just poke poke poke poke poke, grab scalpel, poke n lift n flip n drop, poke n lift n flip n drop: repeat 😂

I’ve seen biopsy tools that have a plunger  but most are designed for one time use.

I made a video but it’s not that good. Soon as I’m done I’ll edit something together. So far the only contams I’ve had are from spores nothing from xfers. That said after watching the vid from my point of view it doesn’t look like i’m hovering over plates but in vid my hand sits over plate too much. Good learning experience, and of course anything that can go wrong will while you’re trying to record it.











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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: Sockadin]
    #26606087 - 04/16/20 03:37 PM (3 years, 9 months ago)

You’d need something like those little plumbing snakes where you push the button and the claws come out the other end.

Then you could just pop the hole punch into the plate and twist so the wedge stays inside, move it to the new plate and push the button down so it pops the wedge out.

lol talk about specialty niche tools.


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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: A.k.a]
    #26606091 - 04/16/20 03:39 PM (3 years, 9 months ago)

Those are good pictures. 

Am I the only one that holds the plate sideways while taking wedges??


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InvisibleD3_Myc
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: A.k.a]
    #26606119 - 04/16/20 03:54 PM (3 years, 9 months ago)

I do both 50/50 but had a camera in my other hand.


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Invisibleeatyualive
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: D3_Myc] * 2
    #26606147 - 04/16/20 04:20 PM (3 years, 9 months ago)

I lift my plates and turn them sideways using the lid as a barrier from things from above. I also use a SAB.


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InvisibleD3_Myc
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: eatyualive]
    #26606176 - 04/16/20 04:39 PM (3 years, 9 months ago)

I started in a SAB, the hood made life much more enjoyable. Now I can take a lid off set to side and take pics or what ever I want to do. Best $400 I’ve spent in a while

I’m probably a little weird for building a hood before ever even getting a harvest. I just enjoy the agar work that much. Only been in hobby since January. Hopefully going to tubs soon with a few.

Speaking of dirty spores.... good thing I streaked a handful





Edited by D3_Myc (04/16/20 04:49 PM)


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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: D3_Myc]
    #26606275 - 04/16/20 05:29 PM (3 years, 9 months ago)

I have to be honest eat. I still open and tilt the lid in the direction of the flowhood take my transfer and do the same with the other lid. I have still gotten drunk during agar work and fucked up some plates because I feel like my FH makes me lazy. Gotta keep that clean work game strong.

I dropped a plate, lid off and it fell upside-down on the floor. Picked it up and used the wedge from it in a jar and started a bottle grow. But this fucking raccoon opened it last week and dumped out a bunch of colonized rye on the back porch. I think he was eating the rye...


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Invisiblestaytrippy420
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: Sockadin]
    #26606314 - 04/16/20 05:43 PM (3 years, 9 months ago)

You’ve never had a harvest D3monic? Damn bruh!
Can’t wait to see some monsters with that sexy camera. You gona kill it :rockon:

Does anyone do anything productive with there dead subs? I’m thinking about making a dead sub mountain in my yard. Mix it with some compost and cover it with coir. Water accordingly and if it doesn’t do shit I’ll just toss it back into the compost pile lol :shrug:

But the slugs and bugs are real bad out here I might just mix it in bins and leave them on my porch. With the nicer weather here I’d like to try something outside.

Oh that reminds me ....
Does anyone have an extra Psilocybe cyanescens print to spare? :begger:


Edited by staytrippy420 (04/16/20 05:49 PM)


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InvisibleD3_Myc
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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: staytrippy420]
    #26606420 - 04/16/20 06:31 PM (3 years, 9 months ago)

Nope, my first jars I fucked up and they dried out. I just used the ring and a filter disc. Surprisingly though I managed to revitalize those jars by PC some distilled water and pouring into the jars. This melmak was completely dry and only 30% colonized. Was going to trash it so said why not try.

Few days later they almost colonized



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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: D3_Myc]
    #26606551 - 04/16/20 07:45 PM (3 years, 9 months ago)

Some PEU from an LC that was a few years old.
Agressive mycelium, but IDK doesn't look like PEU.



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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: Sockadin]
    #26606562 - 04/16/20 07:49 PM (3 years, 9 months ago)

I’ve been saving my old subs the last few weeks cuz supposedly you can grow oysters on them.

We’ll see here pretty soon.


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Re: 🦠🤮VIRUSCATION THREAD🤮🦠 [Re: A.k.a]
    #26606566 - 04/16/20 07:52 PM (3 years, 9 months ago)

You can grow Oysters on spent subs? This would be cool. How do you do this?


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