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OfflineMomorush
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Mycelium in syringe... on purpose?
    #26545656 - 03/19/20 10:57 PM (3 years, 10 months ago)

I remember inspecting my syringes when they were delivered a couple weeks ago and noticed white stuff floating around. I thought it was normal, being my first time and all.
Decided to hit that up in the search and discovered it's actually mycelium and the spores germinated in the syringe. I attribute that to my super fast colonization times (compared to others' experiences I've read here).
I'm just wondering, for when I make my own syringes if it's possible to do it on purpose?  Or is it just luck? Tried searching, but....


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OfflineBikerfool
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Re: Mycelium in syringe... on purpose? [Re: Momorush]
    #26545686 - 03/19/20 11:41 PM (3 years, 10 months ago)

Inoculating with spores isn't really a great  long term strategy as far as contamination is concerned. You could try sterilizing a water, corn syrup mix in the syringe. Mixing  it with spores, drawing back to the  syringe and letting it colonize. There wouldn't be much gas exchange though. Better yet, get some agar and inoculate it with spores. Transfer once and inoculate grain with the wedges.


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Invisiblewoofwoof
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Registered: 01/04/19
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Re: Mycelium in syringe... on purpose? [Re: Bikerfool]
    #26545689 - 03/19/20 11:48 PM (3 years, 10 months ago)

To answe your question, you could just let it germinate in the syringe. I don't know exactly what triggers it, as the only logic seems to be is to let it sit and germinate itself. How old were the syringes you had that contained the myc growth in it?


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OfflineSynKyd
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Re: Mycelium in syringe... on purpose? [Re: woofwoof]
    #26545697 - 03/20/20 12:00 AM (3 years, 10 months ago)

Spores in a clean syringe won’t germinate.  You probably have a bacterial syringe.


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OfflineMomorush
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Re: Mycelium in syringe... on purpose? [Re: SynKyd]
    #26545719 - 03/20/20 12:21 AM (3 years, 10 months ago)

I want to get into agar, but no pressure cooker... yet...
Not sure how old the syringes were before they were sent to me, but I  noticed first day I got them. All 3 of them have wisps of mycelium. I wonder if maybe the vendor sent some lc(li?)...
My jars are coming along nicely. First batch done on the 9th, maybe a few more days until fully colonized.  Looking very healthy, no obvious signs of contamination.


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OfflineMomorush
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Re: Mycelium in syringe... on purpose? [Re: Momorush] * 1
    #26545994 - 03/20/20 07:20 AM (3 years, 10 months ago)

Here's a picture of one of the syringes.  Golden Teacher variety.


Hope it's clear enough. Been in the fridge, so the outside is a little misty.


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OfflineSynKyd
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Re: Mycelium in syringe... on purpose? [Re: Momorush] * 1
    #26546189 - 03/20/20 09:18 AM (3 years, 10 months ago)

If it’s a LC it won’t be cubensis, at least not from a sponsor.


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OfflineMomorush
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Re: Mycelium in syringe... on purpose? [Re: SynKyd]
    #26546203 - 03/20/20 09:24 AM (3 years, 10 months ago)

They're not from a sponsor. Got them from a local vendor. They had good reviews on their Facebook page that, I'd say, are legit.
It's nearly time to fruit them, so time will tell.
I'm feeling optimistic.


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OfflineA.k.aM
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Re: Mycelium in syringe... on purpose? [Re: SynKyd]
    #26546204 - 03/20/20 09:24 AM (3 years, 10 months ago)

Yeah they wouldn’t send that on purpose.

Definitely myc at the bottom there, and like you said for sure sped things up for you.

Look into lcs or li it’s the same thing but on purpose and clean.

I’ve sterilized syringes and sucked up a bunch of myc from an lc, works great.


Just saw your last post, I’m sure it’ll never be an issue but technically those syringes are illegal once the spores germinate and they could get you for mail fraud/distribution/manufacturing.


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Edited by A.k.a (03/20/20 09:26 AM)


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Offlinerumfor69
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Re: Mycelium in syringe... on purpose? [Re: A.k.a]
    #26546238 - 03/20/20 09:36 AM (3 years, 10 months ago)

I got mycelium syringes sent to me once and it was even a sponser here.
I wasn't exactly happy about it. They went into awesome rhizo's two
days after being dropped on agar and are the ones in my signature.


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OfflineSynKyd
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Re: Mycelium in syringe... on purpose? [Re: rumfor69]
    #26546251 - 03/20/20 09:46 AM (3 years, 10 months ago)

Quote:

rumfor69 said:
I got mycelium syringes sent to me once and it was even a sponser here.
I wasn't exactly happy about it. They went into awesome rhizo's two
days after being dropped on agar and are the ones in my signature.




That’s scary, for everyone involved.

What is that third pic in your signature?  Not cubes......I don’t think


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Offlinerumfor69
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Re: Mycelium in syringe... on purpose? [Re: SynKyd]
    #26546269 - 03/20/20 09:52 AM (3 years, 10 months ago)

Quote:

SinysterKyd said:
Quote:

rumfor69 said:
I got mycelium syringes sent to me once and it was even a sponser here.
I wasn't exactly happy about it. They went into awesome rhizo's two
days after being dropped on agar and are the ones in my signature.




That’s scary, for everyone involved.

What is that third pic in your signature?  Not cubes......I don’t think




Yes it was quite scary. They even put what I assume was some charcoal
dust in it to make it look legit. But it clearly had myce all through it.

The third picture was my first attempt at some shiitake blocks. It was
just some cheap genetics I acquired from some random website. Still got
a little over 2 pounds of them off those blocks and a buddy and I stuffed
and covered them with deer meat and cheese.


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OfflineSynKyd
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Re: Mycelium in syringe... on purpose? [Re: rumfor69]
    #26546292 - 03/20/20 10:02 AM (3 years, 10 months ago)

Nice!


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OfflineLogicaL ChaosM
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Re: Mycelium in syringe... on purpose? [Re: Momorush]
    #26546303 - 03/20/20 10:05 AM (3 years, 10 months ago)

Look like an LC syringe. Isnt that illegal? Hmmmmm….


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Offlinerumfor69
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Re: Mycelium in syringe... on purpose? [Re: LogicaL Chaos] * 1
    #26546318 - 03/20/20 10:08 AM (3 years, 10 months ago)

:trollcop:


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Invisiblegoatchild
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Re: Mycelium in syringe... on purpose? [Re: Momorush]
    #26546336 - 03/20/20 10:13 AM (3 years, 10 months ago)

Weeks ago while searching this forum for tips for how to make syringes I remember RR saying something like it is better to use distilled water for syringes so that the spores won't germinate in the water. Maybe that happened cause the water that was used contained enough nutrients for the spores to germinate.

But I don't know why is it a bad thing.


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OfflineMomorush
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Re: Mycelium in syringe... on purpose? [Re: goatchild] * 1
    #26546673 - 03/20/20 01:15 PM (3 years, 10 months ago)

I certainly won't be naming the vendor.
I do recall seeing black specks which I assumed to be spores floating around when I first received them, but now all I see is mycelium. They probably germinated en route.
As long as it's not bacterial, I'm happy.

A question though... anyone know how long these syringes will keep? Got one and a half left, keeping them wrapped in plastic in the fridge.


Edited by Momorush (03/20/20 01:18 PM)


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Offlinerumfor69
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Re: Mycelium in syringe... on purpose? [Re: Momorush] * 1
    #26546773 - 03/20/20 02:01 PM (3 years, 10 months ago)

LoL Now I'm so curious if it's the same vendor :confused2:


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OfflineA.k.aM
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Re: Mycelium in syringe... on purpose? [Re: rumfor69] * 1
    #26547176 - 03/20/20 05:19 PM (3 years, 10 months ago)

Seems like a long time in the fridge, I know lcs can last years according to some.


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OfflineBikerfool
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Re: Mycelium in syringe... on purpose? [Re: A.k.a]
    #26547594 - 03/20/20 08:42 PM (3 years, 10 months ago)

It looks like straight up liquid culture to me.

I've seen small amounts of mycelium in spore syringes but nothing like that.


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