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OfflineMushCat
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Registered: 03/15/20
Posts: 8
Last seen: 3 years, 4 months
Trying to germinate spores on agar, whats wrong with these plates?
    #26545992 - 03/20/20 07:19 AM (3 years, 10 months ago)

Greetings,

I'm new to the shroom cultivation world.

My start was with a GrowKit, which was successfull, I took a spore print from one of the best looking mushrooms and now I've unsuccessfully tried to grow my own mushrooms.

I'm trying to start with agar plates, but I can't seem to germinate my spores.

Here is the process:

I'm using Nutrient Agar from BioLife.

There has been total of three attempts. But the spores don't seem to germinate.

I've tried with just the Nutrient Agar and I've tried with Potatoe flour, Yeast, Honey mixture + Nutrient Agar.

The first IMG doesn't look like mycelium and the second one, I don't eve know. A lot of times, it will be white for a while and then get these dark spots. I'm not even sure wht it is, because it is BELOW the white.





As I said, I'm completely new, and I'm not even sure what I'm looking for.

Are any of the above contain mycelium or is it both cotam?

The process is:

- Create agar mixture
- Sterilize in pressure cooker (15PSI 40 min)
- Pour plates in SAB
- Let ot cool off
- Smear the spores with a loop  in a SAB
- Keep the plates in 79F(26C) controlled incubator

Is the anything wrong with this process?
Could the spores be harvested too soon? (the weil wasn't broken when I harvested the mushroom, I took it off by hand, to harvest spores)
Could there be something wrong with the agar?


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OfflineZenn
LittleLurker
Female User Gallery


Registered: 09/22/19
Posts: 300
Last seen: 1 year, 4 months
Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: MushCat]
    #26546003 - 03/20/20 07:27 AM (3 years, 10 months ago)

Good chance its contaminated. I once had a little white puff on agar I thought mycelium at first but it darkened below the fuzz eventually turning grey.
In another dish I had white start like the 1st pic in agar with mycelium...except this white grew much faster then turned green. The first time I officially met trich.
Up to you but those pics dont look great.

I recommend this. I did these Instead of petri (pastywhyte easy agar):
https://www.shroomery.org/forums/showflat.php/Number/19208976


Edited by Zenn (03/20/20 07:30 AM)


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OfflineMycoBrainz
Mycotic
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Registered: 03/14/15
Posts: 616
Loc: West Coast
Last seen: 15 days, 21 hours
Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: Zenn]
    #26546019 - 03/20/20 07:38 AM (3 years, 10 months ago)

Keep transferring until its clean.


--------------------
:stoned:


PFC x Creeper


Lets Get Stoned


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InvisibleInfiniteDreams
Male

Registered: 10/25/19
Posts: 1,224
Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: Zenn]
    #26546022 - 03/20/20 07:39 AM (3 years, 10 months ago)

I recommend not using a premixed agar.  You want to be able to control the nutrient content.  Try making your own and lowering the nutrient level to 1.5%.


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Offlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,941
Loc: 7b
Last seen: 5 hours, 49 minutes
Trusted Cultivator
Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: Zenn]
    #26546027 - 03/20/20 07:42 AM (3 years, 10 months ago)

I would not recommend going backwards to pasty plates.
Agar is extremely easy. Just read a bit more.Your pics are terrible so we cant help there.
You cannot harvest the spores to soon. Yes there can be something wrong with your agar.
You should have tried to clone one of the mushrooms instead of taking a spore print.

You can practice with grocery store mushrooms.


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,816
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Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: bw86]
    #26546272 - 03/20/20 09:54 AM (3 years, 10 months ago)

That first one looks ok.

Sometimes spore plates look a little wild and need a couple transfers. I only messed with clones at first and when I moved to spores it was a whole different thing. You rarely get nice clean myc right away like with clones.


--------------------
LAGM2020


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 10 hours, 33 minutes
Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: A.k.a]
    #26546281 - 03/20/20 09:58 AM (3 years, 10 months ago)

Quote:

A.k.a said:
That first one looks ok.




:whathesaid:


--------------------

Links For Beginners And Beyond:mspoil:


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OfflineZenn
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Registered: 09/22/19
Posts: 300
Last seen: 1 year, 4 months
Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: rumfor69]
    #26546320 - 03/20/20 10:09 AM (3 years, 10 months ago)

@rumfor69 diggin the profile pic! (Just tried to make mine Radagast but I cant figure out how to edit the size on android device :rolleyes: )


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 10 hours, 33 minutes
Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: Zenn]
    #26546333 - 03/20/20 10:12 AM (3 years, 10 months ago)

Yeah they come out looking bad using the photoshop app.
I had to fire up photoshop CS on my desktop.


--------------------

Links For Beginners And Beyond:mspoil:


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OfflineMushCat
Stranger
Registered: 03/15/20
Posts: 8
Last seen: 3 years, 4 months
Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: A.k.a]
    #26548324 - 03/21/20 08:16 AM (3 years, 10 months ago)

Thank you, I didn't know the fact about spore plates going wild, I thought it needs to look like those pretty ones I see being posted around.

I would have cloned, but when I got my grow kit, I didn't have any supplies as they are not easy to come by where I live.

I took a spore print in case I was going to grow at some point, now I'm doing it 4 months later.


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OfflineAtmozFear
just a shade of myself
Male

Registered: 01/25/19
Posts: 1,032
Last seen: 2 days, 5 hours
Re: Trying to germinate spores on agar, whats wrong with these plates? [Re: rumfor69]
    #26548414 - 03/21/20 09:02 AM (3 years, 10 months ago)

Quote:

rumfor69 said:
Quote:

A.k.a said:
That first one looks ok.




:whathesaid:



same.


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