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owntharabbithole
Stranger

Registered: 03/19/20
Posts: 17
Last seen: 1 month, 15 days
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Looking for agar transfer advice (timing, contams)?
#26544873 - 03/19/20 02:23 PM (3 years, 10 months ago) |
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I've been wanting to start growing again after a few PF Teks in my early 20's. The gist is that I wanted to try stepping up a bit and like many others, love tech & automation and have seen the arguments both for and against automation. Long story short, I want to dabble and work towards a proof of concept. So far I obtained spores from didntreadrules.com, was supposed to get 1 PE syringe and 1 pan cyan Jamaican print, however, they were out of that print so without asking to specify they just sent me the syringe of the cyans too. I've been reading and watching everything I could on the steps to the next level grow and was able to build a pretty badass (IMO) portable flow hood that uses an inline duct fan to get the CFM to 100 (actual CFM 107). I created 4 32qt tubs for fruiting and modified a Crane Drop humidifier to handle all 4 tubs with the use of "t's", although I don't expect it to run all the time.
 To start my flow hood was set up in the doorway of a closet to minimize the airflow around the environment. After spraying and wiping it all down, I used the syringes with pre-poured PDA agar dishes I sourced from Etsy. Next time I'd like to pour my own but I wanted to eliminate at least 1 contam vector and cut my startup work a tiny bit with the plan to make half Penis Envy and half Pan Cyan Jamaican. As for my noob agar procedure lol, I did just one ms drop off of an inoculating loop I made from 18ga SS vape wire that I twisted with a drill and secured in an Exacto knife handle and sterilized. I've never done agar and I def think I need practice so I don't gouge it lol. I don't know if the loop was too big, wire too thin, agar too soft, but when I tried to streak the various lines and patterns it would catch and dig in to the agar. Nonetheless, I flamed the tip in between each streak.
 Currently, for incubation I have all the dishes inside a smaller tub on top of a dry towel, then placed inside one of the 32qt tubs on top of a seedling mat that's controlled by an Inkbird temp/humid controller keeping it within 5 degrees of 83. Currently, there aren't plans for the Inkbird hygrometer; I imagine I could hook a fan up to it but again, I doubt on the small scale it'd be needed much anyway. They have been incubating for 11 days and now im HAPPY to say, I have lots of growth, both good and bad, and likely need to transfer but need to make sure im not grabbing anything im not supposed to lol. The green colonies are obvious but want to be sure the others are mycelium before doing all the work. I thought the pan cyans weren't working but then I spotted the thinner and almost transparent look of it as it starts. Some plates still have absolutely nothing still. Any suggestions or feedback regarding the transfers are greatly appreciated. I've read to make sure they are properly timed, before they hit the edge of the plate, and to look for the most characteristic sections to transfer to the new dishes. I think its time for some and the PE's look decent too, don't they? Im excited and grateful to be a part of this community ;-p
Edited by bodhisatta (03/19/20 04:40 PM)
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owntharabbithole
Stranger

Registered: 03/19/20
Posts: 17
Last seen: 1 month, 15 days
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Re: Looking for agar transfer advice (timing, contams)? [Re: owntharabbithole]
#26545658 - 03/19/20 10:59 PM (3 years, 10 months ago) |
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did I not post this in the correct place?
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HoneyBadger9321
Mushroom Bookie



Registered: 08/07/18
Posts: 64
Last seen: 2 years, 7 months
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Re: Looking for agar transfer advice (timing, contams)? [Re: owntharabbithole]
#26548514 - 03/21/20 09:51 AM (3 years, 10 months ago) |
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Bro that "LFH" looks like it would bring a lot of damn contams. Witch from the pics I can see you have plenty. My thing would be to upgrade to a proper FH. And keep sharpening that teh. down.
-------------------- “Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Heres Tom with the Weather.” ― Bill Hicks
 
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Sunny Skies
Cluster Head


Registered: 05/03/17
Posts: 421
Loc: my house
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Re: Looking for agar transfer advice (timing, contams)? [Re: HoneyBadger9321]
#26551392 - 03/22/20 05:02 PM (3 years, 10 months ago) |
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fuckin hell! you have spent more money on your kit than I have in years..KISS. and KISS some more..good luck though and respect to you and your drive.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Looking for agar transfer advice (timing, contams)? [Re: owntharabbithole] 1
#26551972 - 03/22/20 10:17 PM (3 years, 10 months ago) |
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Quote:
owntharabbithole said: Currently, for incubation I have all the dishes inside a smaller tub on top of a dry towel, then placed inside one of the 32qt tubs on top of a seedling mat that's controlled by an Inkbird temp/humid controller keeping it within 5 degrees of 83.
No no no no no. Keep the temp around high room temp at the most - low 70s. Where you are is where trouble lives.
Next to last pic of PE has the most interesting germinations to me, xfer from those fluffy bits off to the right.
Unfortunately it's a little too common to go the "tech" route when mostly it's not needed. But it's a hobby and you'll learn.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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owntharabbithole
Stranger

Registered: 03/19/20
Posts: 17
Last seen: 1 month, 15 days
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Re: Looking for agar transfer advice (timing, contams)? [Re: PrimalSoup]
#26577643 - 04/04/20 04:21 PM (3 years, 9 months ago) |
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Checking in, havent been able to update much with all the craziness. I tossed the bad plates & picked a couple to transfer, some are getting pretty decent I think but Im still struggling with the jamaican pans. I tried a few more plates out of the syringe, this time with a swab but there all slow fat donuts lol. When would it be ideal to move one to LC?
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slipperyjack66
Scientist


Registered: 02/18/16
Posts: 22
Loc: Middle England
Last seen: 1 year, 2 months
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Re: Looking for agar transfer advice (timing, contams)? [Re: owntharabbithole]
#26588323 - 04/09/20 08:47 AM (3 years, 9 months ago) |
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I'd agree with what was said above, either upgrade to a proper flowhood or just use a still air box. Plenty of people work with agar using a still air box, I did for years with minimal issues. The HEPA filter you're using doesn't look up to scratch, and the fan set up probably isn't producing laminar air flow. There are some good flowhood builds on here to get inspiration from, along with several good glove box/still air box builds. In regards to temperatures, 83 +/-5 is far too high, room temp is fine providing its around 20-24C. You could also build a tub in a tub incubator, they utilise an aquarium heater to keep temps in the interior tub constant. Again, builds can be found on here. Your technique seems solid, unless I'm missing something, so I'd say it's most likely your flowhood or incubation temperatures.
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