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Zombie-ant
Mushroom Ninja in training



Registered: 09/02/19
Posts: 551
Loc: UK
Last seen: 8 days, 12 hours
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Agar plate with myc... but is that cobweb
#26542264 - 03/18/20 08:37 AM (3 years, 10 months ago) |
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Shows how little, I still know,
Is that cobweb (7 and 11 o’clock)

or is that mycelium. It was doing fine...
Boohoo or wahaaay?
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 9 hours, 24 minutes
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Re: Agar plate with myc... but is that cobweb [Re: Zombie-ant] 1
#26542287 - 03/18/20 08:51 AM (3 years, 10 months ago) |
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It looks like just wispy finer fuzzy myce. And cobweb grows super fast. Just transfer from the other ropy rhizo edges and you'll be good. It looks like it's doing great!
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Eclipse3130
Servant of the Fungi



Registered: 10/06/13
Posts: 6,233
Loc: PNW
Last seen: 16 minutes, 19 seconds
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Re: Agar plate with myc... but is that cobweb [Re: rumfor69]
#26542290 - 03/18/20 08:55 AM (3 years, 10 months ago) |
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Looks like a pin mold or cobweb. Watch closely and it will be more obvious here shortly what it is
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
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Zombie-ant
Mushroom Ninja in training



Registered: 09/02/19
Posts: 551
Loc: UK
Last seen: 8 days, 12 hours
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Re: Agar plate with myc... but is that cobweb [Re: Eclipse3130]
#26542296 - 03/18/20 09:02 AM (3 years, 10 months ago) |
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Quote:
rumfor69 said: It looks like just wispy finer fuzzy myce. And cobweb grows super fast. Just transfer from the other ropy rhizo edges and you'll be good. It looks like it's doing great!
Quote:
Eclipse3130 said: Looks like a pin mold or cobweb. Watch closely and it will be more obvious here shortly what it is
Cheers guys... will watch and wait.
I’m about to do some agar on shop bought king oysters and shiitake tissue samples
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Eclipse3130
Servant of the Fungi



Registered: 10/06/13
Posts: 6,233
Loc: PNW
Last seen: 16 minutes, 19 seconds
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Re: Agar plate with myc... but is that cobweb [Re: Zombie-ant]
#26542300 - 03/18/20 09:05 AM (3 years, 10 months ago) |
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Transfer from the other areas in a SAB before it does expand if you want to keep that healthy myc
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
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Zombie-ant
Mushroom Ninja in training



Registered: 09/02/19
Posts: 551
Loc: UK
Last seen: 8 days, 12 hours
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Re: Agar plate with myc... but is that cobweb [Re: Eclipse3130] 1
#26542332 - 03/18/20 09:29 AM (3 years, 10 months ago) |
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Quote:
Eclipse3130 said: Transfer from the other areas in a SAB before it does expand if you want to keep that healthy myc
Yes sir, on it
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 9 hours, 24 minutes
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Re: Agar plate with myc... but is that cobweb [Re: Eclipse3130]
#26542373 - 03/18/20 09:55 AM (3 years, 10 months ago) |
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Quote:
Eclipse3130 said: Transfer from the other areas in a SAB before it does expand if you want to keep that healthy myc
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Zombie-ant
Mushroom Ninja in training



Registered: 09/02/19
Posts: 551
Loc: UK
Last seen: 8 days, 12 hours
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Re: Agar plate with myc... but is that cobweb [Re: rumfor69]
#26542407 - 03/18/20 10:16 AM (3 years, 10 months ago) |
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Quote:
rumfor69 said:
Quote:
Eclipse3130 said: Transfer from the other areas in a SAB before it does expand if you want to keep that healthy myc

Transferred to three other dishes
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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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Re: Agar plate with myc... but is that cobweb [Re: Zombie-ant] 1
#26542433 - 03/18/20 10:31 AM (3 years, 10 months ago) |
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Your speedy turnaround makes me question your sterile technique
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Zombie-ant
Mushroom Ninja in training



Registered: 09/02/19
Posts: 551
Loc: UK
Last seen: 8 days, 12 hours
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Re: Agar plate with myc... but is that cobweb [Re: InfiniteDreams]
#26542468 - 03/18/20 10:53 AM (3 years, 10 months ago) |
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Quote:
InfiniteDreams said: Your speedy turnaround makes me question your sterile technique

I always question my sterile technique too. Using SAB, alcohol dipped and flamed scalpel, Oven sterilised Petri dishes in SAB, stored in tin cans covered in foil. Will see what happens, that will answer your questions, pos or neg will raise some for me always
Aimed at me:
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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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Re: Agar plate with myc... but is that cobweb [Re: Zombie-ant]
#26542509 - 03/18/20 11:18 AM (3 years, 10 months ago) |
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Did you shower recently? Use a mask? Did you use gloves and/or iso on your hands? Did you give time for the air currents to still? Were your movements slow and steady? Did you ever let something unsterile pass above the sterile agar?
Also, IDK about oven sterilization of petris.
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Zombie-ant
Mushroom Ninja in training



Registered: 09/02/19
Posts: 551
Loc: UK
Last seen: 8 days, 12 hours
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Re: Agar plate with myc... but is that cobweb [Re: InfiniteDreams]
#26542547 - 03/18/20 11:43 AM (3 years, 10 months ago) |
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Quote:
InfiniteDreams said: Did you shower recently? Use a mask? Did you use gloves and/or iso on your hands? Did you give time for the air currents to still? Were your movements slow and steady? Did you ever let something unsterile pass above the sterile agar?
Also, IDK about oven sterilization of petris.
Movement and mask... dang
We’ll
Best lessons are the hardest
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