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OfflineBabylon
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Registered: 05/15/11
Posts: 442
Last seen: 2 years, 9 months
How long should it take for contamination to show up on agar?
    #26541586 - 03/17/20 08:49 PM (3 years, 10 months ago)

I'm just getting started and was planning on practicing my SAB agar technique with empty plates.  How long should I expect to wait before something shows up?

Hopefully not too long, I'd like to know if I need to improve, or if I can start innoculating with a reasonable expectation of success before too long.


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OfflineIDX Carnage
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Registered: 03/12/20
Posts: 39
Last seen: 3 years, 10 months
Re: How long should it take for contamination to show up on agar? [Re: Babylon]
    #26542214 - 03/18/20 08:14 AM (3 years, 10 months ago)

I would say go for it either way, if your using a sab properly it will cut down on contam a lot. Remember to have a spray bottle inside to use mist particles to clean air on inside, as wiping down with just iso cleans the sab not the air.

If you do get contam on first agar tray find healthy growth and section off a rice size piece and see if you can get that one 100% clean, if not rinse and repeat.

End result will be you isolating your original culture, but with only the best and most speedy growth. Hope this helps, and if your looking for tips and tricks probably better to search agar and read posts as there are 100’s.


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