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OfflineZenn
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Psilocybe semilanceata mycelium on cakes ...something curious. Pics. Thoughts? * 3
    #26541144 - 03/17/20 04:36 PM (3 years, 10 months ago)

Hi

Just wanted to share a picture of these cakes. Inoculated 1 week ago I think so far they are going well. Considering these have been notoriously difficult for people to grow I'm not sure yet how successful I will be. Never know though maybe my idea will work out. Any success I have I will be sharing :smile: currently I have this experiment and a Ps. Cyanescens experiment. So we shall see how they go...



The cakes are made from a mix of oat bran, vermiculite, meadow grass seed, rabbit droppings and worm castings put in the PC for 90min.


Edited by Zenn (07/27/20 09:53 PM)


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Offlinebw86
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26541182 - 03/17/20 04:58 PM (3 years, 10 months ago)

Is it knocked up with a lc made from a  clean agar culture or home made spore syringe from wild print? Why not make grain spawn?


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Invisiblethe_chosen_one
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: bw86] * 1
    #26541196 - 03/17/20 05:10 PM (3 years, 10 months ago)

They look happy :awesomenod:

Do you intend to fruit from the jar? saralove does her Hispanica this way with great results. My Zapotecorum and Caerulescens do very well this way too.


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: the_chosen_one]
    #26541219 - 03/17/20 05:29 PM (3 years, 10 months ago)

Quote:

bw86 said:
Is it knocked up with a lc made from a  clean agar culture or home made spore syringe from wild print? Why not make grain spawn?




Knocked up with syringe. Got syringe and prints from same place. I was considering grain spawn but opted for cake to start with. I can try from agar etc in future :smile: I'd like to try an LC if this goes well.

Quote:

the_chosen_one said:
They look happy :awesomenod:

Do you intend to fruit from the jar? saralove does her Hispanica this way with great results. My Zapotecorum and Caerulescens do very well this way too.




Well this is where the more experimental part comes in. As these little guys are colonizing I am also pre growing the substrate. I have a tub full of a coir, vermiculite, rabbit droppings and worm castings mix and using that to grow meadow grasses. Since the grass takes about 40 days to die, I knocked up the cakes when the grass germinated. Once the grass dies I am going to mulch the whole box. I will try grating up the fully colonised cakes.
Rough plan atm is to put down half the mulched substrate, then the cake spawn, perhaps damp burlap before pre germinated meadow seed again.

These like a well matured soil and feed on decaying grass roots in meadows and fields by nature so I wanted to try and recreate the type of food they would get. And in nature they tend to start fruiting when the grass starts to die off and it starts getting colder. So that's why I add the germinated grass seed. To try to get the spawn and seeds to grow at the same time and hopefully as the grass is dying they may be fruiting.

I know it might sound like alot of effort but I want to see if it works. If it does then YAY!


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn] * 1
    #26541236 - 03/17/20 05:41 PM (3 years, 10 months ago)

Great experiment! My oldest mentor Bob Harris was really into duplicating environment. It's stuck with me all these years and really taught me how to listen to our little fungal friends.  :sporedrop:


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: the_chosen_one] * 2
    #26541266 - 03/17/20 05:59 PM (3 years, 10 months ago)

Quote:

the_chosen_one said:
Great experiment! My oldest mentor Bob Harris was really into duplicating environment. It's stuck with me all these years and really taught me how to listen to our little fungal friends.  :sporedrop:




Thank you :smile:
That is what I'm hoping to achieve. Maybe they will be grateful and bless me for the environment they are given *crosses fingers *


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26541275 - 03/17/20 06:05 PM (3 years, 10 months ago)

:threadmonitor:


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26546538 - 03/20/20 12:05 PM (3 years, 10 months ago)

Quote:

And in nature they tend to start fruiting when the grass starts to die off and it starts getting colder.



Very true

Quote:

So that's why I add the germinated grass seed.



Where I live, Psilocybe semilanceata grows next to Deschampsia grass


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Hamsabala]
    #26546588 - 03/20/20 12:30 PM (3 years, 10 months ago)

Where I live it is common in fields that often have fescue grasses. I got a meadow seed mix.

What’s in the Mixture
20% Meadow Fescue
10% Tall Fescue
2% Common Bent
15% Timothy


Edited by Zenn (03/20/20 12:31 PM)


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26546628 - 03/20/20 12:54 PM (3 years, 10 months ago)

Quote:

Zenn said:
Hi

Just wanted to share a picture of these cakes. Inoculated 1 week ago I think so far they are going well. Considering these have been notoriously difficult for people to grow I'm not sure yet how successful I will be. Never know though maybe my idea will work out. Any success I have I will be sharing :smile: currently I have this experiment and a Ps. Cyanescens experiment. So we shall see how they go...



The cakes are made from a mix of oat bran, vermiculite, meadow grass seed, rabbit droppings and worm castings put in the PC for 90min.



consolidate and case in the jars, mine fruited ok on regular cakes


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: cronicr]
    #26548126 - 03/21/20 05:18 AM (3 years, 10 months ago)

Thank you Cronicr, I have seen your grows and will take your advice with great consideration.
I am also curious though, what your thoughts are on my experiment idea? If you didn't read that bit:

"have a tub full of a coir, vermiculite, rabbit droppings and worm castings mix and using that to grow meadow grasses. Since the grass takes about 40 days to die, I knocked up the cakes when the grass germinated. Once the grass dies I am going to mulch the whole box. I will try grating up the fully colonised cakes.
Rough plan atm is to put down half the mulched substrate, then the cake spawn, perhaps damp burlap before pre germinated meadow seed again.
To try to get the spawn and seeds to grow at the same time and hopefully as the grass is dying they may be fruiting."

From the growth so far I am unsure how they would do if a cake was grated, the mycelium so far is very fine so may have to rethink that perhaps, if I dont do as you recommend as case in jars once consolidated.


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26548477 - 03/21/20 09:31 AM (3 years, 10 months ago)

yeah for sure trying shit is what paves paths fam. The key to fucking around though is spawn, and lots of it because you are going to fail far more than you succeed.
Getting an understanding of needs and timeframes of the species will be the best tool in your belt so knowing when your grass will die is one step but you also need to get an idea of how your mycelium is colonizing your cakes and then your bulk subs and then how long it takes to see knots and how long those knots can take to turn into pins and how long those pins will take to mature, these are nothing like any tropical species they are slower , more fragile and with multispore it's even more of an unknown as they are not even close to domesticated.
try your experiment but save yourself a cake, considate it for a couple weeks and case it as part of your experiment, my problems in the start with libs was speed , pasteurized subs give about a 2 week window so I prefer sterile substrates for delicate exotics but that's not to say it can not be done.
Good luck fam


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: cronicr]
    #26548480 - 03/21/20 09:34 AM (3 years, 10 months ago)


17 days after I spotted knots


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: cronicr]
    #26551320 - 03/22/20 04:10 PM (3 years, 10 months ago)

Yeah for sure you fail more than succeed with the challenging ones. I have the time (proper hermit!) And luckily I do still have a couple other prints to use and a little still in a syringe from these cakes, pretty sure I have agar somewhere so I do have more to work with just incase.
At least from growing the grass seed now for the bulk, I will get a good idea of how long it will take to die. If lucky the grass and cakes might take roughly the same amount of time, but of course that's just a guess right now.
I'm actually very surprised at the progress of the mycelium so far. I wasnt sure if it would take to the cake but so far so good. A little bigger than the above pic. (Will try upload new one)

Quote:

cronicr said:

17 days after I spotted knots




Oh wow, strange looking.. what happened with that??


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26551397 - 03/22/20 05:04 PM (3 years, 10 months ago)

I'm not sure it just made a cluster of blobs and a couple normalish primodora in the back lol


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: cronicr] * 1
    #26551709 - 03/22/20 08:15 PM (3 years, 10 months ago)

Almost a week since 1st pic, the jars had been moved but think I ligned them up same as before. Also this is how the grass has grown since the day the cakes were inoculated.



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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn] * 1
    #26551733 - 03/22/20 08:27 PM (3 years, 10 months ago)

This pic slightly better:



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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn] * 2
    #26551862 - 03/22/20 09:46 PM (3 years, 10 months ago)

looking good!


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26551882 - 03/22/20 09:52 PM (3 years, 10 months ago)

Quote:

Zenn said:
This pic slightly better:





Those are looking good :super:


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: BooShow]
    #26567524 - 03/30/20 02:57 PM (3 years, 9 months ago)

Roughly a week since the last pics. Lots of growth looking pretty good. mild concern for 1 jar. It's either 3 extra aggressive spots of the mycelium or we may have an invader of a myc of a different shroom. The 3 spots are all at the same height  inbetween the original inoculation points. Makes me think it maybe came from the 5th/middle inoculation point.

Also I may have to rethink the bulk substrate. I might have to PC or pasturize it after the grass has grown as it seems.... the bunny poop I got seems to have spores in it!! I spotted some TINY shroom last night and then checked the bag of poop....to find a nice big white patch of mycelium  in the bag. I did pasturizr the substrate for the grass before but it seems it didn't kill the spores in the poop.




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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26580256 - 04/05/20 07:08 PM (3 years, 9 months ago)

Ok so seems that 1 jar is definitely competing with something else for the substrate. The intruder is very white in comparison to the semilanceata mycelium with slight yellowing. But so far the other 5 are still looking as normal.


A question to anyone that may have tried, is it possible to plant a cake or 2 in a bulk substrate for it to colonize without grating up the cake? Just curious...


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26580378 - 04/05/20 08:01 PM (3 years, 9 months ago)

not a very big sub, it will expand a fe inches but it's slow


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: cronicr]
    #26580439 - 04/05/20 08:31 PM (3 years, 9 months ago)

Noted. Just trying to think what could be the best option for the next stage. As is I'm highly considering casing in the jars with the substrate I'm growing, but PC it before using since it seems to have a grass land mushroom mycelium in it.

Or with the above in mind perhaps a box big enough for 4 or 5 cakes but with only a couple inches around/between each. 

Seeing that it is such a fine mycelium definitely thinking against grating the cakes.


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26620241 - 04/22/20 03:14 PM (3 years, 9 months ago)

I'll keep an eye on this thread! Best of luck !


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Senku] * 1
    #26620400 - 04/22/20 04:11 PM (3 years, 9 months ago)

Pic update. The 3 on the right appear fully colonised. One on the left is almost there, one is still to fill in on the bottom and the 3rd as you can see something else entirely is going on in there. Slight greening in a couple BUT I believe that to be processing the chlorophyll from the grass in the bunny poop since it was very grass green to start with but faded. And appeared in same formation in each it is in. It didn't behave like contamination and the mycelium continued unbothered. Unlike the 1 that is contaminated.that white just took over the jar and infiltrated the fine semilanceata myc that had already grown.



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Re: Psilocybe semilanceata mycelium on cakes ...so far *DELETED* [Re: Zenn] * 2
    #26622664 - 04/23/20 03:26 PM (3 years, 9 months ago)

Post deleted by Senku

Reason for deletion: wrong


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Senku] * 2
    #26622941 - 04/23/20 05:20 PM (3 years, 9 months ago)

Quote:

Zenn said:

...As these little guys are colonizing I am also pre growing the substrate. I have a tub full of a coir, vermiculite, rabbit droppings and worm castings mix and using that to grow meadow grasses. Since the grass takes about 40 days to die, I knocked up the cakes when the grass germinated. Once the grass dies I am going to mulch the whole box....

...These like a well matured soil and feed on decaying grass roots in meadows and fields by nature so I wanted to try and recreate the type of food they would get. And in nature they tend to start fruiting when the grass starts to die off and it starts getting colder. So that's why I add the germinated grass seed. To try to get the spawn and seeds to grow at the same time and hopefully as the grass is dying they may be fruiting.

I know it might sound like alot of effort but I want to see if it works. If it does then YAY!





Quote:

Zenn said:
Where I live it is common in fields that often have fescue grasses. I got a meadow seed mix.

What’s in the Mixture
20% Meadow Fescue
10% Tall Fescue
2% Common Bent
15% Timothy




:smile:


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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26682463 - 05/20/20 02:31 AM (3 years, 8 months ago)

Alright. So an update of sorts. This is technically are going well. I caused 5 of the jars and used grass seeds on top to grow as planned. I used 2Ltr soda bottles with the bottoms cut off for humidity. Grass has grown well. All still looks good.
However it seems I may have run a bit off with my time management as the weather is getting a good bit warmer now. In the "cold" room I have, it gets up to 18°C at times during the day now :frown: some days it's about 15°C. So I'm not sure if the temps are going to be desirable. From what I've read seem to need 15°C or below during the day and 10°C or below during the night...

Which means for both my colder temp experiments I might be a little bit screwed


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26682523 - 05/20/20 04:03 AM (3 years, 8 months ago)

Question to anyone in the know, what are the chances a good cooling fan could lower the temperature enough? It is a small room and mostly made of stone. 2 small windows that get a bit of sun from roughly 2pm or 3pm onwards so heats up more then.
Trying to think of a plan to save these 5 cakes.

Question 2. If I cant get the right temps, can I keep them in the fridge? Or am i basically fucked and better off trying again later in the year?

Excuse the rambling.


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26682725 - 05/20/20 07:16 AM (3 years, 8 months ago)

Id use a portable A/C unit but even that Im not sure it will get cold enough. Might have to fruit inside a modified fridge oe something :strokebeard:

Also very cool project! Ive heard that Liberty Caps are very difficult to cultivate.


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: LogicaL Chaos]
    #26682786 - 05/20/20 08:02 AM (3 years, 8 months ago)

:takingnotes:


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: LogicaL Chaos] * 1
    #26682788 - 05/20/20 08:03 AM (3 years, 8 months ago)

Cheers :smile: I've heard the same but wanted to see if I could. Mini ac unit could maybe work with the fact it's a stone room. Or perhaps a battery powered light might work in the fridge.
To be fair I might be lucky that I got this far on a first attempt haha. Need to get the thinking cap on as the grass is about ready to start dying back so almost time to try set potential fruiting stage


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26683031 - 05/20/20 10:37 AM (3 years, 8 months ago)

I think fridge would be the easiest way to go... When u wake up in the morning put it to the fridge, and when u go to sleep put it in the room under a light.
Then it would have around 4C in the "night" and around 15 on the day time.
Do u have pics? :P


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Senku] * 1
    #26683063 - 05/20/20 10:57 AM (3 years, 8 months ago)

Mini-fridges are great, and you can often find one for cheaper than an AC unit on Craigslist (plus you're using way less power to cool a small space as opposed to a large one).  Really excited to see what comes of these.  I've been dreaming about cultivating these ever since I saw Cronicr's grow(s). :toast:


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: alaskappalachian]
    #26683098 - 05/20/20 11:16 AM (3 years, 8 months ago)

Quote:

alaskappalachian said:
Mini-fridges are great, and you can often find one for cheaper than an AC unit on Craigslist (plus you're using way less power to cool a small space as opposed to a large one).  Really excited to see what comes of these.  I've been dreaming about cultivating these ever since I saw Cronicr's grow(s). :toast:




Can u post a link to that grow?

I will give it a try as well to grow psilocybe semilanceata. I was tripping acid when I got an idea to pick shitloads of liberty caps in the fall and soak them in cold water for some time to get spore water.

And few minutes later we had an idea to get this spore water to turf farm  (place where they grow grass patches) irrigation system to cultivate them that way. So that every1 who buys this turf has some magic hidden in it :smile:


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Senku]
    #26686416 - 05/21/20 07:25 PM (3 years, 8 months ago)



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OfflineZenn
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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: the_chosen_one]
    #26714651 - 06/02/20 04:54 PM (3 years, 7 months ago)

Sorry for the delayed response. I took some pictures of them just now. To be honest I'm not very sure if there is much activity in them. There is condensation, a d the grass is growing but since it was such fine mycelium to start with, it's difficult to tell if it's still alive.


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26806988 - 07/06/20 01:17 PM (3 years, 6 months ago)

How's it going? :smile:


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Senku]
    #26807020 - 07/06/20 01:34 PM (3 years, 6 months ago)

Thanks for asking, I've been meaning to write an update. I think I made a mistake with these little pots. But my mistake was back when I seeded them to grow the grass on top. Noob mistake but I'm sure this is what messed it up.
Before I cased the tops of these to try get to fruiting, I gave them all a little water, as I remember doing that with my other cakes, yet I gave them all too much and didnt realise I'd likely drowned the mycelium before even casing it and growing the grass on top.
BUT
this might not be a complete failure. I could perhaps still use the cakes that had the grass growing through them. None appear to have any contamination. Just looks like in those pictures (since them I did cut the grass so it's much shorter.
I'm wondering since the addition of the extra grass roots, if I could just try again with these cakes? PC them first as it was done before, inoculate cakes again as before but this time do not drown the cakes before going to fruit?

Lol sorry for such a lengthy reply


Edited by Zenn (07/19/20 12:02 AM)


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26831654 - 07/18/20 11:25 PM (3 years, 6 months ago)

Watch this space.

This experiment is not over. Will be restarting in near future. Temperatures will be better soon to do so.

To be continued


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26845255 - 07/25/20 10:05 PM (3 years, 6 months ago)

So I decided to grow grass on top of these again to get more roots into the cakes. I was going to try reuse these cakes after doing so. But here are the pics of some little white formations happening on all 5 cakes. Thoughts on what it could be?



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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26848882 - 07/27/20 09:45 PM (3 years, 5 months ago)

Sorry to be a pest lol, just wondering if anyone thinks it could be the mycelium is still alive and feeding on the old grass roots and grass cuttings?

Any time I trim them I just add the grass cuttings back on top of the cake, there is a very fine whispyness gathering over the clippings, I cant quite get a good enough picture of it to show just yet as it is so fine. It is also hard to find photos of examples of this type of mycelium. Best 2 examples I found are these ( one from erowid, one from shroomery):





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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26849237 - 07/28/20 04:12 AM (3 years, 5 months ago)

I heave no idea, but I really hope it's semilanceata mycelium...

I hope u can pull it off! Best of luck and keep us up to date.


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Senku]
    #26849860 - 07/28/20 12:36 PM (3 years, 5 months ago)

toss in the grass!  isnt wise to reuse the cakes for several reasons so dont do that! if at first it didnt work why would it  if tried again?  tuf to get moisture correct. also contams, spent cakes have less nutrients and mushroom pee.

yes grass seed comes pre inoculated from nature with its symbiotic myco.


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: the man]
    #26850113 - 07/28/20 03:08 PM (3 years, 5 months ago)

Albeit not wise to reuse, mind if I tell you my reasoning for trying it?

due to an error in too much water I added before casing, I wondered if doing same process again but not making the same mistake would work out better (I get it, that's still better to start again entirely. )

Each of the cakes doesnt smell bad, all just smell like dirt like if you dug into the ground.
Also each cake had no visible contamination apart from what is happening on top of the cake. I wondered if this was due to the fact ps. Semilanceata is good at competing or hindering other things:

"Laboratory tests have shown P. semilanceata to suppress the growth of the soil-borne water mold Phytophthora cinnamomi, a virulent plant pathogen that causes the disease root rot. When grown in dual culture with other saprobic fungi isolated from the rhizosphere of grasses from its habitat, P. semilanceata significantly suppresses their growth. This antifungal activity, which can be traced at least partly to two phenolic compounds it secretes, helps it compete successfully with other fungal species in the intense competition for nutrients provided by decaying plant matter."


As for Symbiotic relationship with grass, I did read somewhere it is considered by some to be more of a parasitic type relationship? but I cant currently find where I read that. Unless of course symbiotic and parasitic are the same thing, then that was my misunderstanding.

if it turns out that they really are just done fair enough. I was originally going to throw them out after it failed before, I thought they were dead till these little signs of life. I'm just curious to observe whatever it is I guess.


Edited by Zenn (07/28/20 05:35 PM)


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26850482 - 07/28/20 06:41 PM (3 years, 5 months ago)

What should I search for to find semi agar recipes?
The weak growth on my plates could be nutrient based.


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: smalltalk_canceled]
    #26850522 - 07/28/20 07:03 PM (3 years, 5 months ago)

Hm that's a good question. What about rabbit poop tea agar? Or like use straw (or combo of both) to make a tea, strain with fine filter so you have a clear tea and make agar with that? I know theres been lots of different types of agar recipes like using dog food/kibble etc?

I tried something similar with Ps. Cyanescens on agar made with oak shavings tea, seemed to far prefer that over potato or malt agar.

Just try give it what it likes to eat. Experiments are how we learn :smile:


Edited by Zenn (07/28/20 07:08 PM)


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: Zenn]
    #26851528 - 07/29/20 10:34 AM (3 years, 5 months ago)

Will be making attempts with straw water and grain waiter


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Re: Psilocybe semilanceata mycelium on cakes ...so far [Re: smalltalk_canceled]
    #26852535 - 07/29/20 07:41 PM (3 years, 5 months ago)

Good luck :smile: maybe they will respond better to some straw water


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