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 Actionjunkie
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Registered: 05/05/18
Posts: 200
Last seen: 7 months, 18 days
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 Shoebox pics. Trying to avoid white mold thus round 
#26540340 - 03/17/20 11:10 AM (11 months, 6 days ago) |
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Hi, I had 4 monotubs recently that produced a white mold and no fruit. I thought everything look good until they never pinned or only had a few pins that aborted immediately. So I sent pics of grain spawn (oats) before using them them this time. Also I switched to shoeboxes to isolate each jar of grain so that if there was one bad jar it wouldn't wipe out a mono with 5-6 jars utilized.
Can someone please take a look at these shoeboxes? These pics are after 4 days incubating in the closet at 82°f with the lid left on.
1. Any issues seen in the pics?
2. Should I go ahead and fruit? Or back in the closet to colonize more? (I usually go straight to fruiting without incubation period)
3. If I cleaned cultures on agar (3-4) transfers. Then cut agar wedges to put on the grain. Is it safe to say the issue was my sterile techniques introducing agar to grain or g2g transfer? (I did one g2g transfer before fruiting).
THANK YOU!!!!
J+
         
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Consuming mushrooms may not be addictive but growing them is!
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A.k.a
Stranger
Registered: 10/27/19
Posts: 10,754
Loc: Gaming the system
Last seen: 30 minutes, 45 seconds
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Re: Shoebox pics. Trying to avoid white mold thus round [Re: Actionjunkie]
#26540351 - 03/17/20 11:15 AM (11 months, 6 days ago) |
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I think the last pic looks best.
Lower the temperature down to mid 70s, also I would fruit them.
That myc with the sparse scraggly rhizo fingers always worries me, but sometimes that’s just how it grows.
And yes if your plates were for sure clean then a contamination must’ve come during a2g or g2g.
It takes a little bit to get it down, I had a huge contam rate when I first started dropping wedges and g2g.
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 LAGM2020      
Edited by A.k.a (03/17/20 11:15 AM)
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 LadysKnight
Hello Ladies
Registered: 10/09/15
Posts: 794
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Re: Shoebox pics. Trying to avoid white mold thus round [Re: A.k.a]
#26540467 - 03/17/20 12:17 PM (11 months, 6 days ago) |
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Quote:
A.k.a said:
I think the last pic looks best.
Lower the temperature down to mid 70s, also I would fruit them.
That myc with the sparse scraggly rhizo fingers always worries me, but sometimes that’s just how it grows.
And yes if your plates were for sure clean then a contamination must’ve come during a2g or g2g.
It takes a little bit to get it down, I had a huge contam rate when I first started dropping wedges and g2g.
I agree with this, but will add: I don't think most of us know what a clean plate looks like.
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