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Mycobum7
Stranger


Registered: 01/28/19 
Posts: 180
Last seen: 10 months, 21 days
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guys i fucked up my agar
#26538167 - 03/16/20 08:57 AM (3 years, 10 months ago) |
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im still very new. i recently poured my own agar, 500 ml water to 10 g malt 10 g agar. i poured in front of a hood and a couple days later i have bacteria in at least two agar dishes. I am confident the hood is good because i watched my mentor do transfers in front of it and they are all good, no splotchy bacteria.
I think it is in my pouring tek. off the bat i wasnt fully prepared with a proper agar vessel because my large pc needs a new seal atm so i had to use my small one which meant i used a pint jar to make the agar which meant that pouring it was rather messy.
can someone with experience maybe give me some suggestions? i sterilized the outside of the sleeve and stacked the dishes in front of the hood from bottom to top, mask and gloves on.
im just trying to figure out what part of my tek i need to change, because like i said i watched someone do transfers to plates that succeeded, and i got a significant failure with the same flow hood.
also, what is a good idea of a container for agar for a small pc, i was thinking a small glass vinegar bottle?
-------------------- "This isn't my first rodeo ya know.......its like my third."
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bw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,937
Loc: 7b
Last seen: 1 day, 1 hour
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Re: guys i fucked up my agar [Re: Mycobum7]
#26538178 - 03/16/20 09:01 AM (3 years, 10 months ago) |
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Make sure your hands are behind the plates. Not hovering over or in front. Where a mask. Use any glass bottle you want. I know how much everyone loves RR quotes so i wont post it. But you can read what he said to me years ago here.
Edited by bw86 (03/16/20 09:05 AM)
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Mycobum7
Stranger


Registered: 01/28/19 
Posts: 180
Last seen: 10 months, 21 days
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Re: guys i fucked up my agar [Re: bw86]
#26538193 - 03/16/20 09:10 AM (3 years, 10 months ago) |
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thanks, i went to the post and read it and saved it, RR info is alwasys great sometimes its just hard to find old threads like the one you referred me to during a search, good info about his routine. this will help me. im wondering if maybe i didnt sterilize the handle of the scalpul between transfers and the bacteria came off there.
anyone reading this, im assuming bacteria is like a spore like mold? im going to do a search but if someone sees this and has info on how bacteria moves around that would be cool, but im already off to the searches.
-------------------- "This isn't my first rodeo ya know.......its like my third."
Edited by Mycobum7 (03/16/20 09:10 AM)
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Mycobum7
Stranger


Registered: 01/28/19 
Posts: 180
Last seen: 10 months, 21 days
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Re: guys i fucked up my agar [Re: Mycobum7]
#26538200 - 03/16/20 09:15 AM (3 years, 10 months ago) |
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question: if it appears that the bacteria is growing on top of the agar than is it safe to assume that the bacteria was introduced post pour? as in during the transfer? or would bacteria grow on top if the agar itself got bacteria during the pour after pc?
its funny how these little mistakes open up a whole can of worms in terms of questions
-------------------- "This isn't my first rodeo ya know.......its like my third."
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