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Congame
Stranger
Registered: 02/11/20
Posts: 30
Last seen: 2 years, 4 months
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Try to get mono culture or not (photos included)
#26536735 - 03/15/20 02:01 PM (3 years, 10 months ago) |
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Hi guys,
So a quick background story.... I have been collecting stuff all january and inoculated my first 10 dishes (MEA) on the 31th of january with at 50/50 split between GT and B+
You learn a lot while going at it and my first try got me nowhere but with help from you guys I ditch my incubator and let the dishes sit on top of closet at room temperature, and I re-inoculated my first 10 dishes and made some fresh agar and did another 10 fresh dishes. I did the second inoculation on the 20th of march and after about 5 days I started seeing myc growing on much of the dishes and only contamination on one of them. Initially I didn't plane to do transfers but after reading through a lot of threads it seemed like the way to go and after a few more days I did the first transfer.
This was where I learned my next lesson... Remember to label your dishes... While making transfers from the newest dishes, both GT and B+, I forgot to label them so I didn't knew what was what. I did the old re-inoculated dishes afterwards which I remembered to label  Bummer not to know what was what but then again cube's are cube's I figured so I didn't care to much about it.
I snapped photos of all the plates for you wise guys to take a look at and give me a comprehensive advise a long the way  Some of the dishes wasn't that easy to photograph do to moist in the dishes and being in a zip lock bag...
#1 / B+ (re-inoculated dish)

#1 / GT (re-inoculated dish) Very uniform fluffy growth

#2 / GT (re-inoculated dish) Only fluffy growth

#3 / GT (re-inoculated dish) Fluffy growth almost all the way to the edge, the plate the transfer comes from looked the same actually

#4 / GT (re-inoculated dish)

#1 / Unknown strain

#2 / Unknown strain Only fluffy growth

#3 / Unknown strain

#4 / Unknown strain Only fluffy growth

So know I faced with the next step... Should I try to go straight to grains now or should I try to hit mono culture from making further transfers?! I'm planning to do 8 big tubs so I have also thought about making going to grain with part of the myc and the do some transfer as well, maybe do 2 tubs and then clone the best fruits.?!
Edited by Congame (03/15/20 02:05 PM)
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woofwoof
such mushrooms!



Registered: 01/04/19
Posts: 1,127
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Re: Try to get mono culture or not (photos included) [Re: Congame]
#26536894 - 03/15/20 03:41 PM (3 years, 10 months ago) |
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#3 ? seems like best looking plate to me, except that 9-11 o'clock area. You should do a few transfers, if your doing big tubs, why risk to bad spawn? How many times have these been transferred?
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
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Re: Try to get mono culture or not (photos included) [Re: woofwoof]
#26536902 - 03/15/20 03:55 PM (3 years, 10 months ago) |
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#1 GT
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Babylon
Shaman


Registered: 05/15/11
Posts: 442
Last seen: 2 years, 9 months
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Re: Try to get mono culture or not (photos included) [Re: woofwoof]
#26536906 - 03/15/20 03:58 PM (3 years, 10 months ago) |
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It looks like your question is "should I spawn these or try to isolate them?" and the answer woofwoof gave was "transfer some more to make sure they are clean" which is not one of the options you included.
I'm commenting mostly so I can be sure I understand the conversation so I can learn from it. I appreciate the pics, the moisture and bags may make them harder to see, but they look nice and visible to me. I look forward to seeing how they grow.
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Congame
Stranger
Registered: 02/11/20
Posts: 30
Last seen: 2 years, 4 months
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Re: Try to get mono culture or not (photos included) [Re: Babylon]
#26537105 - 03/15/20 05:44 PM (3 years, 10 months ago) |
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Quote:
woofwoof said: #3 ? seems like best looking plate to me, except that 9-11 o'clock area. You should do a few transfers, if your doing big tubs, why risk to bad spawn? How many times have these been transferred?
Yes defiantly wanted to do a transfer from that one but do I go for multiple small wedges near the edge from 11-9 and put them in each of there own plate?
Quote:
Sockadin said: #1 GT
Why would you choose this particularly one, to my understand (from what I've read) one should go for rhizo growth or is this wrong?
Quote:
Babylon said: It looks like your question is "should I spawn these or try to isolate them?" and the answer woofwoof gave was "transfer some more to make sure they are clean" which is not one of the options you included.
I'm commenting mostly so I can be sure I understand the conversation so I can learn from it. I appreciate the pics, the moisture and bags may make them harder to see, but they look nice and visible to me. I look forward to seeing how they grow.
Yeah thats the question I'm asking  I kind of knew that was the answer but it's also a game of patience but it's hard when you a longing to see those first pins popping up
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Congame
Stranger
Registered: 02/11/20
Posts: 30
Last seen: 2 years, 4 months
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Re: Try to get mono culture or not (photos included) [Re: Congame]
#26537929 - 03/16/20 05:47 AM (3 years, 10 months ago) |
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Actually I was thinking about doing a small tub with one of the dishes I dont plane to clone any, just to get a hang of the process before I scale it up, since it's my first grow and it would be nice to give it a try before it really counts...
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