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hallmaker
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Registered: 01/31/20
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Test to see if Contam or Myc
#26536774 - 03/15/20 02:19 PM (3 years, 10 months ago) |
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Is there any test I can run to see if my petri dishes contains a contam vs myc? I am really trying to hold onto what I believe is myc growing - white, cloudy. Is there any test I can do without growing out to mushrooms to verify?
One other quick one - I am making new petri dishes now. I plan on letting them sit for 3 days to see if any contams pop up. Is 72 hours typically enough? Thanks for the help
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woofwoof
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Registered: 01/04/19
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Re: Test to see if Contam or Myc [Re: hallmaker]
#26536782 - 03/15/20 02:23 PM (3 years, 10 months ago) |
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I usually let them sit for a few days after I make plates. I started keeping test plate (untouched sterile and one where I touch it with my bare hand to see how long or if contams will appear)
Take a small piece and do a transfer to see if its myc or not.
Let the plate grow out a bit first before transferring. I am a newbie at spotting myc and bacteria so I let it grow out a bit first then do a transfer. Sometimes bacteria can look like germinating myc and visa versa so I let it spread a bit then do a transfer and see what it does
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hallmaker
Busy



Registered: 01/31/20
Posts: 75
Loc: Hopefully tripping
Last seen: 2 years, 9 months
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Re: Test to see if Contam or Myc [Re: woofwoof]
#26537506 - 03/15/20 09:06 PM (3 years, 10 months ago) |
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Thanks. Kinda curious, after transfers - can you tell it's a contam most of the time? Have you ever grown out a contam and placed it in a jar for inoculation? At what point do you know?
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PrimalSoup
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Registered: 11/17/09
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Re: Test to see if Contam or Myc [Re: hallmaker]
#26537631 - 03/15/20 10:53 PM (3 years, 10 months ago) |
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There's no point to leaving petris to see if contams grow, because contams grow at different speeds. Just make clean petris. You can leave one alone to see if it contams as a control.
For myc vs contams, (a) experience, (b) myc grows slower than most contams, (c) myc is usually better structured than contams when you examine it.
I have grown out significant amounts once of a contam that I though was myc, but it had some characteristics in retrospect that should have given it away...
If in doubt post pics.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
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Re: Test to see if Contam or Myc [Re: PrimalSoup]
#26537667 - 03/15/20 11:42 PM (3 years, 10 months ago) |
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100% right.
And for sure post pictures next time. We can’t tell a contam from a verbal description.
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