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Offlinepatreides5
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1 week after inoculation, no visible mycelium
    #26536282 - 03/15/20 09:44 AM (3 years, 10 months ago)

As the title says, I inoculated some jars pf Tek style a little over a week ago, no visible growth yet that I see. Maybe the one pictured left shows something?? Temp is probably  75F on average, holes notcovered with tape. PF classic strain
Any suggestions? Thanks in advance



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OfflineC12ShroomMan
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26536287 - 03/15/20 09:48 AM (3 years, 10 months ago)

wait more...start with another week and work your way up :smile:


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: C12ShroomMan]
    #26536301 - 03/15/20 09:54 AM (3 years, 10 months ago)

Thanks for the prompt reply. Last time it took this long it was my first grow and I left the tape on. I should add it was 2cc each jar and waited about 18 hours from sterilizing them before inoculating


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Offlinerumfor69
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26536464 - 03/15/20 11:23 AM (3 years, 10 months ago)

Yeah spores can take up to 3 weeks sometimes in this situation

just hang in there


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Re: 1 week after inoculation, no visible mycelium [Re: rumfor69]
    #26536486 - 03/15/20 11:36 AM (3 years, 10 months ago)

How long did you wait for the jars to cool after PC or steam bath before Inoculation?


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OfflineSurf707
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Re: 1 week after inoculation, no visible mycelium [Re: Sockadin]
    #26536907 - 03/15/20 03:59 PM (3 years, 10 months ago)

My friend did a multi spore run recently. Took him 15 days before he started to see growth.


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Offline6 Speed
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Re: 1 week after inoculation, no visible mycelium [Re: Sockadin]
    #26537038 - 03/15/20 05:07 PM (3 years, 10 months ago)

Quote:

Sockadin said:
How long did you wait for the jars to cool after PC or steam bath before Inoculation?




This is an important piece of info.  Even though a jar may have cooled enough to where you can touch it without burning yourself, if it's still warm, then the contents inside are still very hot.  Too hot for a spore to remain viable.


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OfflineC12ShroomMan
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Re: 1 week after inoculation, no visible mycelium [Re: 6 Speed]
    #26537248 - 03/15/20 07:12 PM (3 years, 10 months ago)

Quote:

6 Speed said:
Quote:

Sockadin said:
How long did you wait for the jars to cool after PC or steam bath before Inoculation?




This is an important piece of info.  Even though a jar may have cooled enough to where you can touch it without burning yourself, if it's still warm, then the contents inside are still very hot.  Too hot for a spore to remain viable.




OP posted 18hrs after, probably fine. I waited 2 days after injected and see nothing after a month, gonna depend on the spores you get too.


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InvisibleTranscendingLife
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Re: 1 week after inoculation, no visible mycelium [Re: C12ShroomMan]
    #26537291 - 03/15/20 07:34 PM (3 years, 10 months ago)

Did you make sure to press the needle against the side of the jar during inoculation?
Because, if you didn’t, you may have growth just not visible yet.
Also, make sure to shake your syringes very well before inoculation to distribute the spores evenly. And don’t use over 1cc per jar, since you may screw up the moisture content, but shouldn’t w/ only 2cc.
Temps sounds fine, time between “sterilization” and inoculation sounds fine.
Are you running 12/12 indirect lighting? That will help speed up colonization a bit as well.


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26543510 - 03/18/20 09:02 PM (3 years, 10 months ago)

Sorry for late response guys, dealing with Corona virus garbage, schools closed, etc. I did press the needle against the side. I'm thinking they're weaker syringes, got them from spore trade.
I waited nearly a day after sterilizing, unlike my first grow where I waited an hour and it took a couple weeks to show signs.
I did just try a couple more jars yesterday, this time with 5cc each, since I think these syringes are weak. I waited about 5 hours after sterilizing this time.

Should they be getting any light? Currently I have them behind a box in top shelf of closet. The light in there is incandescent. Where I fruit has daylight spectrum. I'll post some pics soon.. I think maybe one jar of a couple weeks ago is barely showing life.


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26543521 - 03/18/20 09:08 PM (3 years, 10 months ago)

Attached are some pics, the first I feel like might show the most hope. Each image I show what I feel is barely something.. but maybe wishful thinking :wink:





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Offlineoldest hippie
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26543556 - 03/18/20 09:45 PM (3 years, 10 months ago)

Quote:

patreides5 said:
Attached are some pics, the first I feel like might show the most hope. Each image I show what I feel is barely something.. but maybe wishful thinking :wink:








I think that I see what you are seeing. I presume that you have presented one of the inoculation points.
It is very early, but maybe . I think that you will find out pretty soon.


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Re: 1 week after inoculation, no visible mycelium [Re: oldest hippie]
    #26543590 - 03/18/20 10:04 PM (3 years, 10 months ago)

You can throw off your moisture content by adding too much MS solution. Not likely with that little though.
Give them time, and we’ll find out.
They should be getting indirect 12/12 lighting throughout colonization then direct 12/12 during fruiting.
What temps are you colonizing at? 74 F is about ideal.


--------------------
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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26543617 - 03/18/20 10:18 PM (3 years, 10 months ago)

So is about 12 hours of incandescent fine or should I use my daylight spectrum for it?
Avg temp is about 74-75, I leave the closet door cracked open


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: oldest hippie]
    #26543619 - 03/18/20 10:19 PM (3 years, 10 months ago)

Thanks oldest hippie, yes that was my goal for each :wink:


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InvisibleTranscendingLife
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26543624 - 03/18/20 10:24 PM (3 years, 10 months ago)

Quote:

patreides5 said:
So is about 12 hours of incandescent fine or should I use my daylight spectrum for it?
Avg temp is about 74-75, I leave the closet door cracked open




I use a 6500K bulb just like I do for fruiting.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26543638 - 03/18/20 10:35 PM (3 years, 10 months ago)

Gotcha. I had been using 5000K in previous 2 grows so I'll just use those instead and leave them on every night


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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26543651 - 03/18/20 10:43 PM (3 years, 10 months ago)

Make sure that your lights are on the same 12/12 schedule as far as mycelium growth and fruiting. Circadian rhythm and all.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26543657 - 03/18/20 10:48 PM (3 years, 10 months ago)

Will do! That's usually what I have done. Thanks a bunch for the help, I'll keep y'all posted <3


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26550668 - 03/22/20 10:35 AM (3 years, 10 months ago)

Curious:
- what is the longest I should wait before disposing of the jars? I'm reading old threads about some taking 2 months!
- I'm seeing in old threads some people would turn the jars upside down when they stalled. Is that a deprecated practice now?

I'm considering wrapping their sides in small rags to see if the additional heat helps


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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26550772 - 03/22/20 11:23 AM (3 years, 10 months ago)

I was having this exact problem around the same time. Now I'm seeing fuzzy growth lined around the bottom edges. Be patient.


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Offline6 Speed
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5] * 1
    #26550835 - 03/22/20 11:49 AM (3 years, 10 months ago)

Quote:

patreides5 said:
Curious:
- what is the longest I should wait before disposing of the jars? I'm reading old threads about some taking 2 months!
- I'm seeing in old threads some people would turn the jars upside down when they stalled. Is that a deprecated practice now?

I'm considering wrapping their sides in small rags to see if the additional heat helps




Best advice is honestly to simply set them aside and forget about them for a while. If you want another project to occupy your mind while you wait, you could buy some more spore syringes from a different vendor and inoculate some additional jars. Maybe you'll have faster progress from another vendor's product. You could also begin your journey into agar. You could build a flow hood. Plenty of other mushroom things to get involved in while you wait.


Edited by 6 Speed (03/22/20 11:50 AM)


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: SunMnstr]
    #26550839 - 03/22/20 11:51 AM (3 years, 10 months ago)

Yeah it's driving me crazy. I feel like if I got the spores from spore works they would've been nearly done by now. I have several prints from my first grow that I may use to do agar soon


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26551758 - 03/22/20 08:48 PM (3 years, 10 months ago)

Is this contam?



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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26551761 - 03/22/20 08:51 PM (3 years, 10 months ago)

Bottom left of the picture or dead center?
Either way it’s extremely hard to tell.
Leave the jar alone and quit fiddling with it for a few days. Then you’ll be able to tell.


--------------------
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"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26551767 - 03/22/20 08:54 PM (3 years, 10 months ago)

Either but mostly dead center. Will do


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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26551771 - 03/22/20 08:56 PM (3 years, 10 months ago)

Yea, give it a few days. And you’ll know.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26552702 - 03/23/20 09:30 AM (3 years, 10 months ago)

Safe to say this is growth I think!:cool:



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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26552939 - 03/23/20 11:36 AM (3 years, 10 months ago)

I don't think I can see it yet, keep hanging in there! 😎


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: rumfor69]
    #26560721 - 03/27/20 08:36 AM (3 years, 9 months ago)

Well the good news is I notified the vendor of concern for the syringe viability and they're sending replacements. If I don't see much more in a couple weeks I may dump these in a garden pot in my garden to free up more jars


Edited by patreides5 (03/27/20 08:45 AM)


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26563330 - 03/28/20 02:05 PM (3 years, 9 months ago)

Is this contam?



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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26563348 - 03/28/20 02:16 PM (3 years, 9 months ago)

Yes


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Re: 1 week after inoculation, no visible mycelium [Re: PiptheGreAtest]
    #26563376 - 03/28/20 02:31 PM (3 years, 9 months ago)

Bye bye jar. Time for another try :shrug:
We all get contams


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26563382 - 03/28/20 02:33 PM (3 years, 9 months ago)

Yup. I only had 1 my first grow after like second flush. Already secluded that one away before you guys responded :/


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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26569946 - 03/31/20 08:07 PM (3 years, 9 months ago)

W00t. Nearly a month later finally some noticable mycelium. The pics are 2 different jars





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Offlinepoisoned
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26570337 - 04/01/20 03:07 AM (3 years, 9 months ago)

It doesn't look like cube mycelium sadly


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: poisoned]
    #26570413 - 04/01/20 05:31 AM (3 years, 9 months ago)

You think that's contamination?


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Offlinepoisoned
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26570418 - 04/01/20 05:35 AM (3 years, 9 months ago)

Yeah, but wait for someone else to jump in before throwing anything away


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Re: 1 week after inoculation, no visible mycelium [Re: poisoned]
    #26570449 - 04/01/20 06:12 AM (3 years, 9 months ago)

Yeah I felt like it's mycelium but it has been a few months since my last grow


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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26570531 - 04/01/20 07:20 AM (3 years, 9 months ago)

Started a new thread to see if it's contam, one feedback says it looks like mycelium
https://www.shroomery.org/forums/showflat.php/Number/26570526/vc/1#26570526


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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26570551 - 04/01/20 07:34 AM (3 years, 9 months ago)

It looks like some kind of wispy mold to me. Try to remember how you inoculated, normally you push the needle up against the glass, past the dry verm layer. So good myc is usually starting in a line kind of and growing out from there.

The pattern of this growth looks like it started from spores that got in and just past the dry verm layer so you see that half circle coming down from the top.

It’s possible that it’s thin cube myc maybe but if that showed up at that size over night you know it’s mold.


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Re: 1 week after inoculation, no visible mycelium [Re: Roger Clemency]
    #26570633 - 04/01/20 08:23 AM (3 years, 9 months ago)

On this post just focusing on the growth https://www.shroomery.org/forums/showflat.php/Number/26570606/vc/1#26570606
I have at least one comment saying it looks like mycelium. FWIW, those 2 jars were almost a month old and had displayed what I would consider weak growth


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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26571163 - 04/01/20 12:52 PM (3 years, 9 months ago)

Looks a bit light to be mushroom mycelium, imho.


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AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
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:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26571618 - 04/01/20 04:56 PM (3 years, 9 months ago)

Ah, just posted on thread for the growth if a trusted cultivator could weigh in. Ugh, really hard to decide to toss, maybe I just move away from my usual grow space for a couple days. I just want so desperately to believe after all this time I gained victory 😭


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InvisibleTranscendingLife
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Re: 1 week after inoculation, no visible mycelium [Re: patreides5]
    #26571966 - 04/01/20 08:15 PM (3 years, 9 months ago)

Set it aside for a day or so. :shrug:
We all get contams. Check out my gallery


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AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26572138 - 04/01/20 09:39 PM (3 years, 9 months ago)

Yeah. I did get one so far that even my newbie self knew was contam.
I just inoculated some new jars tonight. Golden teacher and galindoi. If the galindoi succeeds, I may just try to fruit them for prints instead of truffles since I can't do grain right now.


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Offlinepatreides5
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Re: 1 week after inoculation, no visible mycelium [Re: TranscendingLife]
    #26579216 - 04/05/20 11:02 AM (3 years, 9 months ago)

Dumped that contam jar in an old pot of soil, mixed, added dead leaves, watered.. thinking I'm seeing the beginnings/pin of a fruit here 😅



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