Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   Mushroom-Hut Substrate Mix   Original Sensible Seeds Autoflowering Cannabis Seeds

Jump to first unread post Pages: 1
OfflineClc420
Stranger
 User Gallery

Registered: 06/24/18
Posts: 276
Last seen: 11 months, 15 days
Isolating clones
    #26535978 - 03/15/20 05:22 AM (3 years, 10 months ago)

So when you clone a fruiting body you can isolate sectors and transfer until you get monocultures of each sector. Then fruit them out to see how they grow. Is it actually worth isolating sectors or can you just grow out the original clone mycelium?


Extras: Filter Print Post Top
Offlinemagicschoolbus
I'm a teapot User Gallery


Registered: 02/19/18
Posts: 160
Loc: Shroomery Flag
Last seen: 8 months, 15 days
Re: Isolating clones [Re: Clc420]
    #26536003 - 03/15/20 05:58 AM (3 years, 10 months ago)

Sure,

let's say you clone a fruit tissue and end up with a culture with say, 5 clear sectors.

Why not transfer out each sector, put each transfer to 2 grain jars, then grow 5 x 2quart shoeboxes

Then you can use the same substrate recipe and grain prep tek, and you can measure the end weight of each shoebox.

Then, once you've discovered the most successful culture, return to the culture on agar, expand it again, and save a transfer in a master slant.

To me this is the coolest part of Mycology

If you don't wanna #doitfortheculture then yeah you could just put the original sectored clone culture to grain and get the same results, but that wouldn't be nearly as fun


--------------------
:trippinballs::trippinballs:


Edited by magicschoolbus (03/15/20 05:59 AM)


Extras: Filter Print Post Top
OfflineClc420
Stranger
 User Gallery

Registered: 06/24/18
Posts: 276
Last seen: 11 months, 15 days
Re: Isolating clones [Re: magicschoolbus]
    #26536177 - 03/15/20 08:42 AM (3 years, 10 months ago)

Quote:

magicschoolbus said:
Sure,

let's say you clone a fruit tissue and end up with a culture with say, 5 clear sectors.

Why not transfer out each sector, put each transfer to 2 grain jars, then grow 5 x 2quart shoeboxes

Then you can use the same substrate recipe and grain prep tek, and you can measure the end weight of each shoebox.

Then, once you've discovered the most successful culture, return to the culture on agar, expand it again, and save a transfer in a master slant.

To me this is the coolest part of Mycology

If you don't wanna #doitfortheculture then yeah you could just put the original sectored clone culture to grain and get the same results, but that wouldn't be nearly as fun





I totally agree with you just curious on why it's best to isolate. I'm super excited to work through a clone and find a winner. Same as popping seeds growing cannabis and finding the best pheno.

I've got my hepa coming for my flow hood so that will be built soon, cant wait to be self sufficient by having my own isolates and constantly grow!


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 14 seconds
Trusted Cultivator
Re: Isolating clones [Re: Clc420]
    #26536197 - 03/15/20 08:59 AM (3 years, 10 months ago)

Isolate isn’t always best, unless you happen to get great genetics. The more isolated the more consistent and uniform the shrooms will be, but you can end up getting a lot of slow/low yield/weak potency cultures too.

Idk if this is the correct way to look at it, but I think of ms as an average of all the genetics so it’ll almost always be decent potency and speed. When you only grab one set of genes its still likely to be pretty average, but way more likely than ms to be extremely above or below.


--------------------
LAGM2020


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   Mushroom-Hut Substrate Mix   Original Sensible Seeds Autoflowering Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* differences betwen strain isolation by transfers and by cloning?
( 1 2 3 4 all )
FGL 14,940 62 10/06/09 08:13 PM
by feelfunny
* Do you isolate strains? Liquidkick 4,151 9 07/21/06 10:14 AM
by RogerRabbit
* rhizomorphic isolate going back to linear / tomentose ohmatic 4,650 15 01/19/05 01:47 PM
by tripndicular
* Is there a Poor Man's way to ISOLATE?
( 1 2 3 all )
SHROOMpimp 6,557 49 04/13/05 12:49 PM
by Hotnuts
* Strain Isolation from Syringe: check my procedure.
( 1 2 all )
Omnicracker 3,752 23 07/28/06 10:56 PM
by Roadkill
* whens the best time to isolate the strain on agar? A_Phlux 676 4 11/18/06 08:06 PM
by RogerRabbit
* agar to agar transfer ohmatic 15,620 11 01/12/21 09:00 PM
by bodhisatta
* Explain to me the joys of substrain isolation (karo jars etc) IfIWereARichMan 2,135 14 03/24/10 04:09 PM
by rberg79

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
152 topic views. 17 members, 181 guests and 46 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.009 seconds on 14 queries.