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OfflineZenn
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Ps. Cyanescens Indoors. Mulch box season 2. still alive.... disaster strikes
    #26535887 - 03/15/20 03:09 AM (3 years, 10 months ago)

Hi there :smile:

Got a little experiment going, and from a little research it seems I may have some tomentose mycelium growth? Ps. Cyanescens from a surprise agar germination, to popcorn grain spawn, to layered mulch box. Curious if the tomentose growth is a good sign? Or if it is indeed that. Everything seems to look real fluffy like mini snow mounds. Everything smells alright, I think/hope it's going well lol



Edited by Zenn (07/24/21 06:23 PM)


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Invisibletramalot
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Re: Tomentose mycelium? Ps. Cyanescens mulch box [Re: Zenn]
    #26535890 - 03/15/20 03:13 AM (3 years, 10 months ago)

Kinda looks like it wants to Blob!

But does look clean but I am new

Good luck


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OfflineAtmozFear
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Re: Tomentose mycelium? Ps. Cyanescens mulch box [Re: Zenn]
    #26535990 - 03/15/20 05:46 AM (3 years, 10 months ago)

that looks super clean to me!!  Are all Ps. Cyanescens like that at this stage (someone else can tell me)?


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Edited by AtmozFear (03/15/20 05:46 AM)


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OfflineZenn
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Re: Tomentose mycelium? Ps. Cyanescens mulch box [Re: AtmozFear]
    #26537733 - 03/16/20 01:02 AM (3 years, 10 months ago)

Thanks. Tbh I've been quite lucky getting this to this point. I've also never seen such fluffy growth before! So just wanted to be sure it was ok. I guess time will tell.


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn] * 1
    #26541243 - 03/17/20 05:44 PM (3 years, 10 months ago)

Here are a couple more pics of the formation development. I find it really interesting. So fluffy



The mulch (aimed for high lignin from more than chips) :
Dried oak leaves
Oak chips
Oak bark
Oak sawdust
Coir
Vermiculite
Oat bran
Tortoise food "meadow mix" (dried readi grass, nettle, dandelions other meadow greens and flowers).

"Casing":
Coir
Worm castings
Vermiculite
Pine cone bits
Pine bark
Pine needles
Mixed dried leaves (oak, birch, beech)

The wood etc was boiled and the dried leaves and grass etc was pasteurized then they were mixed together. I did try to case it with a layer of worm casting, coir, pine cone bits, pine needles, mixed dried leaves like it would have in a forest area but it ate it's way through that too haha.

I wonder if I should put a layer damp vermiculite on top once its fully colonised the top..


Edited by Zenn (03/17/20 07:17 PM)


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OfflineA.k.aM
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26541281 - 03/17/20 06:07 PM (3 years, 10 months ago)

There’s a whole thread on pans.

I’m just starting them myself, I was told to isolate thinner wispy growth on agar, because super puffy myc tends to do crazy overlay and never fruit.

But it looks like with all those rhizo fingers it should pin.

What are you fruiting it in?


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: A.k.a]
    #26541290 - 03/17/20 06:15 PM (3 years, 10 months ago)

Quote:

A.k.a said:
There’s a whole thread on pans.

I’m just starting them myself, I was told to isolate thinner wispy growth on agar, because super puffy myc tends to do crazy overlay and never fruit.

But it looks like with all those rhizo fingers it should pin.

What are you fruiting it in?




These are not pans they are psilocybe cyanescens. A wood lover. So far colonizing aggressively in an unmodified tub. I'm trying to do them indoor but replicating an environment they may have in nature. Also right now in this country it's cold enough to keep in my pantry or in a room if the window is open


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26543811 - 03/19/20 02:20 AM (3 years, 10 months ago)

Decided to put a layer of vermiculite over the mycelium since it's basically a big white brick now. I will be very pleased if I manage to get this to fruit. I have it in my pantry next to open window, which is between 7°C and 10°C most of the time. At night gets down to 5°C or below. We shall see if the fruit favours its growing conditions as much as the mycelium did.


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Invisiblec10h12n2o
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26543818 - 03/19/20 02:36 AM (3 years, 10 months ago)

Hope it works out for you! I've never had much luck with wood lovers indoors

I've always had them colonize aggressively but never had any luck with the fruiting indoors


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: c10h12n2o]
    #26543852 - 03/19/20 03:23 AM (3 years, 10 months ago)

Thanks :smile: yeah haha apparently they are a challenge. I just have a sort of gut feeling for the direction I took up till this point.

Including while they were colonizing before I "cased" they were in temps of 23°c to 26°c range. Then after casting  were kept in another room at between 10 and 15°C. Did an ice mist using a humidifier, straining bag and ice. I did move them to the pantry briefly before, then I realised it was consuming the casing more than anticipated likely due to the worm castings. So did take it back into the 10 to 15 temp room until the pics above. Its now got a layer of vermiculite to try and keep the moisture level and I think today the temps are below 10°C which should be hopefully good temps for primordia (if I remember correctly from waylitjim's posts).

I guess I am up for a challenge as I am currently trying this indoor and Psilocybe semilanceata indoor started on cakes. If I can get these projects to work I will be very happy :laugh:


Edited by Zenn (03/19/20 04:21 AM)


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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26543882 - 03/19/20 03:58 AM (3 years, 10 months ago)

Send a brother a print if you succeed w either !!


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: c10h12n2o]
    #26543899 - 03/19/20 04:21 AM (3 years, 10 months ago)

Noted :P


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26543944 - 03/19/20 05:59 AM (3 years, 10 months ago)

Quote:





I see 2 different types of mycelia/ Don't you think that it is going to overlay?

I wish you will get great experience and harvest. :thumbup:
And i wish to get few prints too :rolleyes:

I am not sure if you know growing parameters of Ps. Cyanescens... just in case:

Spawn Run:
Incubation Temperature: 65-75° F. (18-24° C.)
Relative Humidity: 95-100%
Duration: 45-60 days
C02: >5 000 ppm
FreshAir Exchanges: 0-1
Light Requirements: n/a

Primordia Formation:
Initiation Temperature: 45-55° F. (7-13° C.)
Relative Humidity: 95-100%
Duration: 10-14 days
C02: 1000-2000 ppm
Fresh Air Exchanges: 2-4 or as needed.
Light Requirements: 400-800 lux.

Fruitbody Development:
Temperature: 50-65° F. (10-18° C.)
Relative Humidity: 90-95%
Duration: 10-20 days.
C02: 1000-2000 ppm
Fresh Air Exchanges: 2-4 or as needed
Light Requirements: 400-800 lux.

Cropping Cycle:
2 crops, 3-4 weeks apart.


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26543993 - 03/19/20 06:51 AM (3 years, 10 months ago)

Quote:

Hamsabala said:
Quote:





I see 2 different types of mycelia/ Don't you think that it is going to overlay?

I wish you will get great experience and harvest. :thumbup:
And i wish to get few prints too :rolleyes:

I am not sure if you know growing parameters of Ps. Cyanescens... just in case:

Spawn Run:
Incubation Temperature: 65-75° F. (18-24° C.)
Relative Humidity: 95-100%
Duration: 45-60 days
C02: >5 000 ppm
FreshAir Exchanges: 0-1
Light Requirements: n/a

Primordia Formation:
Initiation Temperature: 45-55° F. (7-13° C.)
Relative Humidity: 95-100%
Duration: 10-14 days
C02: 1000-2000 ppm
Fresh Air Exchanges: 2-4 or as needed.
Light Requirements: 400-800 lux.

Fruitbody Development:
Temperature: 50-65° F. (10-18° C.)
Relative Humidity: 90-95%
Duration: 10-20 days.
C02: 1000-2000 ppm
Fresh Air Exchanges: 2-4 or as needed
Light Requirements: 400-800 lux.

Cropping Cycle:
2 crops, 3-4 weeks apart.





Yes thank you I was aware of temp ranges :smile: I found waylitjim fall cyanescens thread on another site.
Thank you for adding it, always good to have more notes. And the light info very useful input.

As for the mycelium formations I am quite unfamiliar with it. So please tell me about overlay?

Basically the story of this mulch box was I had been trying to get grain spawn with little success. One day I noticed an old malt agar plate I tried to germinate spore on MONTHS previously, I was going to throw it out as I thought it inactive, it now had white fuzz. So I watched and it soon took off on the agar just by pure luck I reckon. It sure took it's time! I had less Impressive growth on other potato/wood agar that was older (I still have them just incase) but decided to use this plate. Out of the jars I managed to knock up from it this was the healthiest growth by far and surprisingly never contaminated like all the rest.

Its growth although fluffy I have observed it to be heavily rhizomorphic as its eaten it's way through the mulch. Little fingers always greedily grabbing.
It seems where the sort of hexagonal shapes are it's like that's rhizomes? That have met? But I am unfamiliar with this kind of formation so any advice I will gladly listen to.
I may have bitten off more than I can chew for my level of experience, but I want to try best I can to help these little dudes fruit seeing as they just randomly sprung to life on a previously thought dead agar... I took it as a sign hahaha


Edited by Zenn (03/19/20 06:53 AM)


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26544025 - 03/19/20 07:10 AM (3 years, 10 months ago)

Here is where I keep it right now. I have a komodo hygrometer/thermometer attached to the lid. The sides of the box are lightly raised by folded gloves to allow air. (Not the most glamorous I know haha but its clean)



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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26544096 - 03/19/20 07:52 AM (3 years, 10 months ago)

Quote:

Zenn said:
As for the mycelium formations I am quite unfamiliar with it. So please tell me about overlay?





Overlay
A condition of the casing where mycelium been allowed to completely cover the surface. Overlay is caused by prolonged vegetative growth temperatures, high CO2 levels and excessive humidity. Overlay, if overwatered, becomes matted.


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26544109 - 03/19/20 07:59 AM (3 years, 10 months ago)

Ah, ok thank you. I didn't know about this. How do you think it will be effected by the thin layer of vermiculite I added to the top? It was almost fully white on the top but I put about 1cm of vermiculite  on last night..


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26544154 - 03/19/20 08:18 AM (3 years, 10 months ago)

Quote:

Zenn said:
Ah, ok thank you. I didn't know about this. How do you think it will be effected by the thin layer of vermiculite I added to the top? It was almost fully white on the top but I put about 1cm of vermiculite  on last night..




Looks like time to form primordia and fruiting then.
I never use vermiculite. So i don't know.
Overlay can affect further fruiting and decrease one.
I believe, that hardwood sawdust and wood chips, especially alder, oak, birch, and beech are better for supporting fruiting.


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Edited by Hamsabala (03/19/20 08:20 AM)


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26544170 - 03/19/20 08:23 AM (3 years, 10 months ago)

This is true. I have been using mostly oak. However I wasnt sure if I add more wood, if it would just try to eat that too like it did with the casing. Or would perhaps coir and bark be better I wonder as I did think they needed something chunky to grow next to/from


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26544196 - 03/19/20 08:43 AM (3 years, 10 months ago)

According Paul Stamets
"Heliotropic and primarily preferring deciduous woods, especially in riparian habitats, these mushrooms fruit in the fall. Possibly a saprophyte of fir seed cones, they show a particular fondness for the decorative mulch ("beauty bark") used in landscaping around newly constructed buildings."

So mulch | sawdust | wood chips + soil | peat | coir for cover layer looks good.


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