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Syntax.Error
Ringmaster


Registered: 11/20/19
Posts: 33
Last seen: 3 years, 5 months
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Contam or Early MYC? PICS
#26535650 - 03/14/20 10:43 PM (3 years, 10 months ago) |
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These were nocced up about 4 days ago. Kinda new to agar so was wondering if everything looks ok?
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Syntax.Error
Ringmaster


Registered: 11/20/19
Posts: 33
Last seen: 3 years, 5 months
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Any opinions?
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Syntax.Error
Ringmaster


Registered: 11/20/19
Posts: 33
Last seen: 3 years, 5 months
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I guess I am going to assume these are contams and not Myc from the spores i Nocced with.
Just curious I have made a couple post asking for help and never get a reply? How does one go about getting opinions from some of the TCs or more advanced forum users?
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IDX Carnage
Sir


Registered: 03/12/20
Posts: 39
Last seen: 3 years, 9 months
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No expert but it’s nice to get some feedback...
Looks okay to me, if I was guessing did you inoculate agar from syringe?
If so cut a wedge near edge with strong growth and start a new agar plate if so and you my get more defined rhizo.... just repeat until satisfied..
If you have area of concern can you take better photo and mark it up where your concerned?
Hope this helps
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Syntax.Error
Ringmaster


Registered: 11/20/19
Posts: 33
Last seen: 3 years, 5 months
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Re: Contam or Early MYC? PICS [Re: IDX Carnage]
#26538427 - 03/16/20 11:37 AM (3 years, 10 months ago) |
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Pretty much with the whole area. It doesnt look feathery like other agars I have seen pictured. I dropped spores from a print onto the agar in a SAB. The weird circle structures are throwing me off as there isnt any defined "Flow" to the myc.
These are my first attempts at agar and all 12 look the same. but doesnt look like anything i have seen pictured.
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IDX Carnage
Sir


Registered: 03/12/20
Posts: 39
Last seen: 3 years, 9 months
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So this is how I understand it okay but it may be wrong
Whey you use spores your using tons and tons of possible genetic combinations which is why people get worked over the word strain... cuz if you inbox a brf cake from a syringe you may get 100 of strains.....
So when you innoc agar from this many possible genetic combos you won’t have isolation of many let alone 1 strain.... this is why you should take a grain size piece from a vain of rhizo and transfer to a new plate and let that grow out and repeat.
There is an entire thread on agar you should read on here as it’s helpful. Also google search agar clone isolation and I am sure you will get good information that can help you progress
The blobs and growth you see is likely the cause of competing gentetics and “mutants”
So my final advice really is the same take strong healthy grain size piece and start new tray... rinse repeat!
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Mr Solo Dolo


Registered: 02/24/19
Posts: 243
Loc: U.S
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Is the growth covering the whole plate already? Looks a bit wierd to me.. 4 days also seems pretty fast in my experience..
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IDX Carnage
Sir


Registered: 03/12/20
Posts: 39
Last seen: 3 years, 9 months
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Okay this will help you tons, use this link and read bods tek. Also read strohs.... I think it will answer all you need
https://www.shroomery.org/forums/showflat.php/Number/22721954
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IDX Carnage
Sir


Registered: 03/12/20
Posts: 39
Last seen: 3 years, 9 months
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Re: Contam or Early MYC? PICS [Re: IDX Carnage]
#26538462 - 03/16/20 11:59 AM (3 years, 10 months ago) |
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RR is also insanely knowledgeable and I just saw there are links to videos I will be checking those out I would say do the same. Let us know if this helped and if your questions are answered.
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Syntax.Error
Ringmaster


Registered: 11/20/19
Posts: 33
Last seen: 3 years, 5 months
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Re: Contam or Early MYC? PICS [Re: IDX Carnage]
#26538557 - 03/16/20 12:45 PM (3 years, 10 months ago) |
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Correct within 4 days the growth covers the top of the agar. The spores are less than a month old if that matters to the speed that much but i fear not.
Tad of history.
Bought a syringe from a not so good source. Nocced up some boom bags. had about 2 good flushes. broke the bags into shoe boxes with coir had a couple more flushes.
https://www.shroomery.org/forums/showflat.php/Number/26478290#26478290
Took prints from the fruits. Now I am looking to learn from those prints before working with some RustyWhyte prints I have and I would like to start with agar. Pretty much did the pastywhyte agar substituting the gladware with half pint jars.
And yep been reading for a few months now (not sure what teks I have not read LOL I have fallen in love with mycology) but just havent seen anything that looks like this in what is posted here or anywhere. I just dont want to start doing transfers and such if this is indeed a contam and not the expected Myc. Would kind of defeat my purpose to start learning agar with a contam and not actual myc.
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IDX Carnage
Sir


Registered: 03/12/20
Posts: 39
Last seen: 3 years, 9 months
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do you have any shrooms you could just clone from. If so use sanitary part that is in very middle of stem. This is what I plan to do.
Also that’s kind of the wonderful thing about agar even if it’s contaminated you can cut a clean section and start a new tray..... maybe if you try to get the cleanest best part possible the growth will have an area that’s great and then section and tray that piece.... rinse and repeat, worst case scenario you grow another tray of bacteria .... do it for science:)
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Syntax.Error
Ringmaster


Registered: 11/20/19
Posts: 33
Last seen: 3 years, 5 months
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Re: Contam or Early MYC? PICS [Re: IDX Carnage]
#26538613 - 03/16/20 01:17 PM (3 years, 10 months ago) |
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Here are some top down pics
It is all white as a fresh snow, there are just some shadows.
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IDX Carnage
Sir


Registered: 03/12/20
Posts: 39
Last seen: 3 years, 9 months
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Much better photos, yea that is funky looking, someone smarter than me will be able to much better assist you with an answer now, I still say do another plate for science maybe near the very edge on the top right. Good luck!!
Part of agar is seeing and understanding contamination so hey if your learning something what’s the harm. That or you have so insanely strong and prominent mycelium ha
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Mr Solo Dolo


Registered: 02/24/19
Posts: 243
Loc: U.S
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Re: Contam or Early MYC? PICS [Re: IDX Carnage]
#26538767 - 03/16/20 02:50 PM (3 years, 10 months ago) |
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I would toss that.. doesn't look good
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Kimble
Idiot

Registered: 03/08/18
Posts: 508
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Quote:
Syntax.Error said: Here are some top down pics
It is all white as a fresh snow, there are just some shadows.


Mine look like this.
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Syntax.Error
Ringmaster


Registered: 11/20/19
Posts: 33
Last seen: 3 years, 5 months
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Any clue what it could be just for shits and giggles? I have been searching all over and cant find anything that looks like it haha
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SynKyd
ctrl-alt-delite



Registered: 09/27/13
Posts: 1,554
Loc: ૐ
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Bacterial probably
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Syntax.Error
Ringmaster


Registered: 11/20/19
Posts: 33
Last seen: 3 years, 5 months
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Re: Contam or Early MYC? PICS [Re: SynKyd]
#26540270 - 03/17/20 08:37 AM (3 years, 10 months ago) |
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Thanks for the input will be tossing and restarting.
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