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Offlinepsycho_nauticus
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Significant difference in isopropyl alcohol?
    #26535162 - 03/14/20 04:30 PM (3 years, 10 months ago)

With this Coronavirus thing I was only able to get 91% instead of 70%. Is there any change or significant difference with using the 91%?


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InvisiblebodhisattaMDiscordReddit
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Re: Significant difference in isopropyl alcohol? [Re: psycho_nauticus] * 2
    #26535169 - 03/14/20 04:36 PM (3 years, 10 months ago)

Dilute it down to 70% then its fine.


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Offlinerumfor69
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Re: Significant difference in isopropyl alcohol? [Re: bodhisatta]
    #26535181 - 03/14/20 04:43 PM (3 years, 10 months ago)

I only use 91% :shrug:


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Offlinepsycho_nauticus
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Re: Significant difference in isopropyl alcohol? [Re: bodhisatta]
    #26535190 - 03/14/20 04:49 PM (3 years, 10 months ago)

Is it a necessity that I have to? Is there anything the 90% one will affect and can’t do that the 70% one does?


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Re: Significant difference in isopropyl alcohol? [Re: psycho_nauticus] * 2
    #26535192 - 03/14/20 04:50 PM (3 years, 10 months ago)



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Offlinerumfor69
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Re: Significant difference in isopropyl alcohol? [Re: Strainsfordaze]
    #26535198 - 03/14/20 04:56 PM (3 years, 10 months ago)

Hmm news to me. I've only ever used 91% and haven't seen a contaminated
jar or tub in years. Only ever see contams if I'm using a ms syringe on
some agar to start some new genetics.


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OfflinecronicrFacebook
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Re: Significant difference in isopropyl alcohol? [Re: rumfor69] * 2
    #26535207 - 03/14/20 05:04 PM (3 years, 10 months ago)

Quote:

rumfor69 said:
Hmm news to me. I've only ever used 91% and haven't seen a contaminated
jar or tub in years. Only ever see contams if I'm using a ms syringe on
some agar to start some new genetics.



technique trumps iso any day of the week


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Re: Significant difference in isopropyl alcohol? [Re: cronicr] * 3
    #26535213 - 03/14/20 05:06 PM (3 years, 10 months ago)

open a plate in open air, when you see green on the plate squirt a syringe full of iso at any percent on it and suck it up, then squirt it in a grain jar and see for yourself just how magic it actually isnt


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Offlinerumfor69
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Re: Significant difference in isopropyl alcohol? [Re: cronicr]
    #26535220 - 03/14/20 05:16 PM (3 years, 10 months ago)

My thoughts exactly. I remember a famous Rabbit here that I believe
once said "As long as your jars are sterile, your mycelium is transferred clean,
and your substrate is pasteurized correctly, you can pick your butt while
putting things together and it will probably be fine."

or something to that extent lol


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InvisibleStipe-n CapMDiscord
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Re: Significant difference in isopropyl alcohol? [Re: rumfor69]
    #26535237 - 03/14/20 05:30 PM (3 years, 10 months ago)

He's also the same guy that drowns his receiving jars in iso during his g2g video in "let's grow mushrooms "


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Offlinerumfor69
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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap]
    #26535240 - 03/14/20 05:33 PM (3 years, 10 months ago)

I also do that, and not so much the butt picking thing lmao


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Re: Significant difference in isopropyl alcohol? [Re: cronicr]
    #26535241 - 03/14/20 05:34 PM (3 years, 10 months ago)

Quote:

cronicr said:
open a plate in open air, when you see green on the plate squirt a syringe full of iso at any percent on it and suck it up, then squirt it in a grain jar and see for yourself just how magic it actually isnt




If it's ineffective why do people use it...do you use it?


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OfflineA.k.aM
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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap]
    #26535246 - 03/14/20 05:38 PM (3 years, 10 months ago)

I think it’s like a motorcycle helmet, it might save you if you screw up, but you’re better off not crashing in the first place.


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Re: Significant difference in isopropyl alcohol? [Re: A.k.a]
    #26535253 - 03/14/20 05:42 PM (3 years, 10 months ago)

I don't always use my jars right away but need to use the pc. Sometimes the jars sit on the shelf and collect dust. You wouldn't wash them with iso before opening in front of the hood?


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Offlinerumfor69
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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap]
    #26535258 - 03/14/20 05:44 PM (3 years, 10 months ago)

I think we're getting off topic from the OP...possible threadjacking in progress lol


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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap] * 1
    #26535279 - 03/14/20 05:56 PM (3 years, 10 months ago)

Quote:

p9hu7 said:
Quote:

cronicr said:
open a plate in open air, when you see green on the plate squirt a syringe full of iso at any percent on it and suck it up, then squirt it in a grain jar and see for yourself just how magic it actually isnt




If it's ineffective why do people use it...do you use it?



I'm not saying it's ineffective it's just not a huge crutch , it's a disinfectant for molds and bacteria but not super effective on spores, I see people daily using iso in their sab  I stead of soap and water or they use Lysol or bleach but you can be just as effective if not more with soap and water and not have to work in a fire hazard, jars and shit can get wiped down because you don't want to saturate your filters and iso evaporates quickly which is also why it's not great for sab prep


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OfflinecronicrFacebook
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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap] * 1
    #26535282 - 03/14/20 05:58 PM (3 years, 10 months ago)

Quote:

p9hu7 said:
He's also the same guy that drowns his receiving jars in iso during his g2g video in "let's grow mushrooms "



brings up another good point, there are big differences between working with a sab and a flowhood:super:


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Re: Significant difference in isopropyl alcohol? [Re: cronicr]
    #26535304 - 03/14/20 06:14 PM (3 years, 10 months ago)

Thanks, Cron.


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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap] * 1
    #26535323 - 03/14/20 06:25 PM (3 years, 10 months ago)

Is hydrogen peroxide better than ipa for sanitation?


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Re: Significant difference in isopropyl alcohol? [Re: woofwoof]
    #26535385 - 03/14/20 07:08 PM (3 years, 10 months ago)

Hydrogen peroxide ain't no good at all.


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Re: Significant difference in isopropyl alcohol? [Re: InfiniteDreams] * 1
    #26535410 - 03/14/20 07:24 PM (3 years, 10 months ago)

91% just evaporates faster. Sometimes too fast.


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Re: Significant difference in isopropyl alcohol? [Re: Errl_Shmirl]
    #26535461 - 03/14/20 08:08 PM (3 years, 10 months ago)

CDC - https://www.cdc.gov/infectioncontrol/guidelines/disinfection/disinfection-methods/chemical.html

In the healthcare setting, β€œalcohol” refers to two water-soluble chemical compoundsβ€”ethyl alcohol and isopropyl alcoholβ€”that have generally underrated germicidal characteristics 482. FDA has not cleared any liquid chemical sterilant or high-level disinfectant with alcohol as the main active ingredient. These alcohols are rapidly bactericidal rather than bacteriostatic against vegetative forms of bacteria; they also are tuberculocidal, fungicidal, and virucidal but do not destroy bacterial spores. Their cidal activity drops sharply when diluted below 50% concentration, and the optimum bactericidal concentration is 60%–90% solutions in water.

Mode of Action.

The most feasible explanation for the antimicrobial action of alcohol is denaturation of proteins. This mechanism is supported by the observation that absolute ethyl alcohol, a dehydrating agent, is less bactericidal than mixtures of alcohol and water because proteins are denatured more quickly in the presence of water


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Re: Significant difference in isopropyl alcohol? [Re: woofwoof] * 3
    #26535544 - 03/14/20 08:55 PM (3 years, 10 months ago)

Quote:

woofwoof said:
Is hydrogen peroxide better than ipa for sanitation?



IPA is a little weak for a sanitizer I'd drink it instead. Lmao before someone comes and trys to correct me I should say I'm only joking. I don't drink IPAs.


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OfflineBabylon
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Re: Significant difference in isopropyl alcohol? [Re: gizmo1]
    #26535643 - 03/14/20 10:35 PM (3 years, 10 months ago)

How about bleach?  Or Iodine?


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OfflineLogicaL ChaosM
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Re: Significant difference in isopropyl alcohol? [Re: Babylon]
    #26535729 - 03/15/20 12:11 AM (3 years, 10 months ago)

cronicr mentioned he uses bleach/Lysol for cleaning his SAB.

Yeah, IPA is a terrible beer and disinfectant! :lol:


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InvisibleTranscendingLife
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Re: Significant difference in isopropyl alcohol? [Re: Errl_Shmirl]
    #26535758 - 03/15/20 12:44 AM (3 years, 10 months ago)

Quote:

Errl_Shmirl said:
91% just evaporates faster. Sometimes too fast.




not sometimes... all the time.
You have the correct answer, Sir.
70% has enough water in it that it penetrates cell walls of bacteria and mold. 91% does not.


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Re: Significant difference in isopropyl alcohol? [Re: cronicr]
    #26536020 - 03/15/20 06:12 AM (3 years, 10 months ago)

Quote:

cronicr said:
Quote:

p9hu7 said:
Quote:

cronicr said:
open a plate in open air, when you see green on the plate squirt a syringe full of iso at any percent on it and suck it up, then squirt it in a grain jar and see for yourself just how magic it actually isnt




If it's ineffective why do people use it...do you use it?



I'm not saying it's ineffective it's just not a huge crutch , it's a disinfectant for molds and bacteria but not super effective on spores, I see people daily using iso in their sab  I stead of soap and water or they use Lysol or bleach but you can be just as effective if not more with soap and water and not have to work in a fire hazard, jars and shit can get wiped down because you don't want to saturate your filters and iso evaporates quickly which is also why it's not great for sab prep




bod said the other day that he doesn't even use soapy water anymore to prep his SAB... he said he leaves the walls dry with nothing.  Maybe you guys can debate?  I am just interested in learning.


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Edited by AtmozFear (03/15/20 06:13 AM)


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Re: Significant difference in isopropyl alcohol? [Re: AtmozFear]
    #26536195 - 03/15/20 08:58 AM (3 years, 10 months ago)

So why bother saying anything, I wasn't talking about a SAB I was asking about the effectiveness of 70% iso when sanitizing surfaces. People have been making comments lately that make it sound like they don't use iso at all, which seemed strange. Cron cleared it up for me.


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OfflineA.k.aM
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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap]
    #26536210 - 03/15/20 09:07 AM (3 years, 10 months ago)

The guys that use dry boxes are just saying that technique is what matters. Iso and soapy water are there as a back up in case you make a mistake, but if you know what you’re doing and can do it flawlessly on a regular basis then you don’t really need the safety net.


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Re: Significant difference in isopropyl alcohol? [Re: A.k.a]
    #26536218 - 03/15/20 09:13 AM (3 years, 10 months ago)

Well I don't use a SAB, I have a flow hood. The example I used was that I often have sterilized jars laying around on a shelf collecting dust. Opening them in front of the hood without spraying and whipping them doesn't sound like reliance on a crutch at all, it sounds like common sense. That was my issue. Bod said that he doesn't use it and that sometimes he flames the neck of his jars when I know for sure that he uses a flow hood.
I would certainly still clean those shelved jars even if I was using a SAB.

Eats seem to agree that a dry SAB may increase rates of contamination, so I wouldn't call soap a crutch either, but hey :shrug:

Ultimately I was just trying to get to the bottom of this whole no use of ISO thing.


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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap]
    #26536226 - 03/15/20 09:16 AM (3 years, 10 months ago)

I used a sab a lot longer than ive had a hood. The outside of jars is always dirty. Even wiped down. Technique saves you tho.

I use a sab at my own home still for any mycology endeavors.


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Re: Significant difference in isopropyl alcohol? [Re: bodhisatta]
    #26536232 - 03/15/20 09:19 AM (3 years, 10 months ago)

So you wouldn't wipe your jars under those circumstances when using a hood?


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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap]
    #26536239 - 03/15/20 09:22 AM (3 years, 10 months ago)

Mason jar lids cover the threads. You take the lid off you have a clean jar neck anyway. Like i said sometimes i flame the thread/neck area but meh


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Re: Significant difference in isopropyl alcohol? [Re: bodhisatta]
    #26536255 - 03/15/20 09:29 AM (3 years, 10 months ago)

Ok, thanks for your input.


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Offlinepsycho_nauticus
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Re: Significant difference in isopropyl alcohol? [Re: TranscendingLife]
    #26536697 - 03/15/20 01:45 PM (3 years, 10 months ago)

Well I got lucky today and found the last bottle in a store, and it was 70%!!! I never realized how much I go through when having to clean and wipe down my SAB.

But I feel like using 90% isn't going to make a difference if it came down to me having to use it. They both disinfect and will get you to the cleanliness you need.


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Re: Significant difference in isopropyl alcohol? [Re: AtmozFear]
    #26536874 - 03/15/20 03:25 PM (3 years, 10 months ago)

Quote:

LogicaL Chaos said:
cronicr mentioned he uses bleach/Lysol for cleaning his SAB.

Yeah, IPA is a terrible beer and disinfectant! :lol:



I kind of worded it funny I don't use Lysol or bleach just soapy water, I will sometimes disinfect my sab an hour or so before I use it but mostly I just use soap and water , things get wet and contams don't move it's a pretty basic principle and it works for me so I'm not the least bit worried about iso not being on shelves right now.
Quote:

AtmozFear said:
Quote:

cronicr said:
Quote:

p9hu7 said:
Quote:

cronicr said:
open a plate in open air, when you see green on the plate squirt a syringe full of iso at any percent on it and suck it up, then squirt it in a grain jar and see for yourself just how magic it actually isnt




If it's ineffective why do people use it...do you use it?



I'm not saying it's ineffective it's just not a huge crutch , it's a disinfectant for molds and bacteria but not super effective on spores, I see people daily using iso in their sab  I stead of soap and water or they use Lysol or bleach but you can be just as effective if not more with soap and water and not have to work in a fire hazard, jars and shit can get wiped down because you don't want to saturate your filters and iso evaporates quickly which is also why it's not great for sab prep




bod said the other day that he doesn't even use soapy water anymore to prep his SAB... he said he leaves the walls dry with nothing.  Maybe you guys can debate?  I am just interested in learning.



there isn't much to debate, he also does unmodded tubs where I stick to classic monos but the end goal is the same


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Re: Significant difference in isopropyl alcohol? [Re: cronicr]
    #26536890 - 03/15/20 03:39 PM (3 years, 10 months ago)

By classic do you mean poly or MP tape?


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Re: Significant difference in isopropyl alcohol? [Re: Sockadin]
    #26536891 - 03/15/20 03:40 PM (3 years, 10 months ago)

Holes in general


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Re: Significant difference in isopropyl alcohol? [Re: bodhisatta]
    #26536991 - 03/15/20 04:45 PM (3 years, 10 months ago)

Most SAB cleaning can be done with just soap & bleach or Lysol ECT.

Save ISO for scaple\loop hands and jars. A qt should last 100-200 jars bags or plates easy


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Re: Significant difference in isopropyl alcohol? [Re: tramalot]
    #26537313 - 03/15/20 07:40 PM (3 years, 10 months ago)

How many oz of distilled water do I need to add to 16oz of 91% to get it to around 70%?
Is there a formula I can use to figure % I suck at math.
If I mix 50% and 70% 50/50 what percentage will I get.

Can I get a crash course in figuring percentages?


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Re: Significant difference in isopropyl alcohol? [Re: gizmo1]
    #26537342 - 03/15/20 07:51 PM (3 years, 10 months ago)

Quote:

gizmo1 said:
Can I get a crash course in figuring percentages?




https://www.calkoo.com/en/solution-dilution-calculator


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OfflineA.k.aM
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Re: Significant difference in isopropyl alcohol? [Re: gizmo1]
    #26537349 - 03/15/20 07:54 PM (3 years, 10 months ago)

I want to say 7 parts water to 4 parts alcohol but idk.

I figure if it was 100% you’d go 7 to 3, so 10% more alcohol is 3.3 parts...

Now I’m lost.


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Edited by A.k.a (03/15/20 07:56 PM)


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Re: Significant difference in isopropyl alcohol? [Re: gizmo1]
    #26537396 - 03/15/20 08:17 PM (3 years, 10 months ago)

Quote:

gizmo1 said:
If I mix 50% and 70% 50/50 what percentage will I get.




I'll go out on a limb here and say 60%.  (It's right in the middle...)

Quote:

gizmo1 said:
How many oz of distilled water do I need to add to 16oz of 91% to get it to around 70%?
Is there a formula I can use to figure % I suck at math.

Can I get a crash course in figuring percentages?




Trick here is the dilution has to be calculated first.  what is the alcohol to water?  16oz of 91%  is how much alcohol to water?  And the alcohol volume will stay the same.  (it is just 16 * .91)

16oz of 91% iso = 14.56oz of iso and 1.44oz of H2O.

How much total volume will you have for 70% iso?  This is 14.56 / .7 = 20.8 oz

But remember, you were not pure, you already have 1.44oz of H2O there.  So you need to add 20.8 - 14.56 - 1.44 = 4.8oz of high quality H2O.


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InvisibleTranscendingLife
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Re: Significant difference in isopropyl alcohol? [Re: InfiniteDreams]
    #26537429 - 03/15/20 08:35 PM (3 years, 10 months ago)

:header:


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InvisibleInfiniteDreams
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Re: Significant difference in isopropyl alcohol? [Re: TranscendingLife]
    #26537459 - 03/15/20 08:50 PM (3 years, 10 months ago)

Make it simpler.  Need 20.8oz.  Have 16oz.  Add 4.8oz of H2O.


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Invisiblegizmo1
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Re: Significant difference in isopropyl alcohol? [Re: TranscendingLife]
    #26537487 - 03/15/20 09:00 PM (3 years, 10 months ago)

Quote:

InfiniteDreams said:
Make it simpler.  Need 20.8oz.  Have 16oz.  Add 4.8oz of H2O.



If that's not simple enough round up.
Need 21oz. Have 16. Add 5oz.
Lol thanks ID. :thumbup:
Quote:

TranscendingLife said:
:header:



Gor some reason I feel like I just saw a ghost. Lol how ya been TL?


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OfflineNoisecontrol
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Re: Significant difference in isopropyl alcohol? [Re: gizmo1]
    #26616060 - 04/20/20 06:51 PM (3 years, 9 months ago)

TL.... o_o thought you died.  :thatistroubling:


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