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InvisibleErrl_Shmirl
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Re: Significant difference in isopropyl alcohol? [Re: InfiniteDreams] * 1
    #26535410 - 03/14/20 07:24 PM (3 years, 10 months ago)

91% just evaporates faster. Sometimes too fast.


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Invisiblemurderlabz
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Re: Significant difference in isopropyl alcohol? [Re: Errl_Shmirl]
    #26535461 - 03/14/20 08:08 PM (3 years, 10 months ago)

CDC - https://www.cdc.gov/infectioncontrol/guidelines/disinfection/disinfection-methods/chemical.html

In the healthcare setting, “alcohol” refers to two water-soluble chemical compounds—ethyl alcohol and isopropyl alcohol—that have generally underrated germicidal characteristics 482. FDA has not cleared any liquid chemical sterilant or high-level disinfectant with alcohol as the main active ingredient. These alcohols are rapidly bactericidal rather than bacteriostatic against vegetative forms of bacteria; they also are tuberculocidal, fungicidal, and virucidal but do not destroy bacterial spores. Their cidal activity drops sharply when diluted below 50% concentration, and the optimum bactericidal concentration is 60%–90% solutions in water.

Mode of Action.

The most feasible explanation for the antimicrobial action of alcohol is denaturation of proteins. This mechanism is supported by the observation that absolute ethyl alcohol, a dehydrating agent, is less bactericidal than mixtures of alcohol and water because proteins are denatured more quickly in the presence of water


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Invisiblegizmo1
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Re: Significant difference in isopropyl alcohol? [Re: woofwoof] * 3
    #26535544 - 03/14/20 08:55 PM (3 years, 10 months ago)

Quote:

woofwoof said:
Is hydrogen peroxide better than ipa for sanitation?



IPA is a little weak for a sanitizer I'd drink it instead. Lmao before someone comes and trys to correct me I should say I'm only joking. I don't drink IPAs.


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OfflineBabylon
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Re: Significant difference in isopropyl alcohol? [Re: gizmo1]
    #26535643 - 03/14/20 10:35 PM (3 years, 10 months ago)

How about bleach?  Or Iodine?


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OfflineLogicaL ChaosM
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Re: Significant difference in isopropyl alcohol? [Re: Babylon]
    #26535729 - 03/15/20 12:11 AM (3 years, 10 months ago)

cronicr mentioned he uses bleach/Lysol for cleaning his SAB.

Yeah, IPA is a terrible beer and disinfectant! :lol:


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InvisibleTranscendingLife
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Re: Significant difference in isopropyl alcohol? [Re: Errl_Shmirl]
    #26535758 - 03/15/20 12:44 AM (3 years, 10 months ago)

Quote:

Errl_Shmirl said:
91% just evaporates faster. Sometimes too fast.




not sometimes... all the time.
You have the correct answer, Sir.
70% has enough water in it that it penetrates cell walls of bacteria and mold. 91% does not.


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OfflineAtmozFear
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Re: Significant difference in isopropyl alcohol? [Re: cronicr]
    #26536020 - 03/15/20 06:12 AM (3 years, 10 months ago)

Quote:

cronicr said:
Quote:

p9hu7 said:
Quote:

cronicr said:
open a plate in open air, when you see green on the plate squirt a syringe full of iso at any percent on it and suck it up, then squirt it in a grain jar and see for yourself just how magic it actually isnt




If it's ineffective why do people use it...do you use it?



I'm not saying it's ineffective it's just not a huge crutch , it's a disinfectant for molds and bacteria but not super effective on spores, I see people daily using iso in their sab  I stead of soap and water or they use Lysol or bleach but you can be just as effective if not more with soap and water and not have to work in a fire hazard, jars and shit can get wiped down because you don't want to saturate your filters and iso evaporates quickly which is also why it's not great for sab prep




bod said the other day that he doesn't even use soapy water anymore to prep his SAB... he said he leaves the walls dry with nothing.  Maybe you guys can debate?  I am just interested in learning.


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Edited by AtmozFear (03/15/20 06:13 AM)


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InvisibleStipe-n CapMDiscord
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Re: Significant difference in isopropyl alcohol? [Re: AtmozFear]
    #26536195 - 03/15/20 08:58 AM (3 years, 10 months ago)

So why bother saying anything, I wasn't talking about a SAB I was asking about the effectiveness of 70% iso when sanitizing surfaces. People have been making comments lately that make it sound like they don't use iso at all, which seemed strange. Cron cleared it up for me.


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OfflineA.k.aM
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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap]
    #26536210 - 03/15/20 09:07 AM (3 years, 10 months ago)

The guys that use dry boxes are just saying that technique is what matters. Iso and soapy water are there as a back up in case you make a mistake, but if you know what you’re doing and can do it flawlessly on a regular basis then you don’t really need the safety net.


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InvisibleStipe-n CapMDiscord
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Re: Significant difference in isopropyl alcohol? [Re: A.k.a]
    #26536218 - 03/15/20 09:13 AM (3 years, 10 months ago)

Well I don't use a SAB, I have a flow hood. The example I used was that I often have sterilized jars laying around on a shelf collecting dust. Opening them in front of the hood without spraying and whipping them doesn't sound like reliance on a crutch at all, it sounds like common sense. That was my issue. Bod said that he doesn't use it and that sometimes he flames the neck of his jars when I know for sure that he uses a flow hood.
I would certainly still clean those shelved jars even if I was using a SAB.

Eats seem to agree that a dry SAB may increase rates of contamination, so I wouldn't call soap a crutch either, but hey :shrug:

Ultimately I was just trying to get to the bottom of this whole no use of ISO thing.


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InvisiblebodhisattaMDiscordReddit
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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap]
    #26536226 - 03/15/20 09:16 AM (3 years, 10 months ago)

I used a sab a lot longer than ive had a hood. The outside of jars is always dirty. Even wiped down. Technique saves you tho.

I use a sab at my own home still for any mycology endeavors.


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InvisibleStipe-n CapMDiscord
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Re: Significant difference in isopropyl alcohol? [Re: bodhisatta]
    #26536232 - 03/15/20 09:19 AM (3 years, 10 months ago)

So you wouldn't wipe your jars under those circumstances when using a hood?


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InvisiblebodhisattaMDiscordReddit
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Re: Significant difference in isopropyl alcohol? [Re: Stipe-n Cap]
    #26536239 - 03/15/20 09:22 AM (3 years, 10 months ago)

Mason jar lids cover the threads. You take the lid off you have a clean jar neck anyway. Like i said sometimes i flame the thread/neck area but meh


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InvisibleStipe-n CapMDiscord
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Re: Significant difference in isopropyl alcohol? [Re: bodhisatta]
    #26536255 - 03/15/20 09:29 AM (3 years, 10 months ago)

Ok, thanks for your input.


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Offlinepsycho_nauticus
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Re: Significant difference in isopropyl alcohol? [Re: TranscendingLife]
    #26536697 - 03/15/20 01:45 PM (3 years, 10 months ago)

Well I got lucky today and found the last bottle in a store, and it was 70%!!! I never realized how much I go through when having to clean and wipe down my SAB.

But I feel like using 90% isn't going to make a difference if it came down to me having to use it. They both disinfect and will get you to the cleanliness you need.


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OfflinecronicrFacebook
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Re: Significant difference in isopropyl alcohol? [Re: AtmozFear]
    #26536874 - 03/15/20 03:25 PM (3 years, 10 months ago)

Quote:

LogicaL Chaos said:
cronicr mentioned he uses bleach/Lysol for cleaning his SAB.

Yeah, IPA is a terrible beer and disinfectant! :lol:



I kind of worded it funny I don't use Lysol or bleach just soapy water, I will sometimes disinfect my sab an hour or so before I use it but mostly I just use soap and water , things get wet and contams don't move it's a pretty basic principle and it works for me so I'm not the least bit worried about iso not being on shelves right now.
Quote:

AtmozFear said:
Quote:

cronicr said:
Quote:

p9hu7 said:
Quote:

cronicr said:
open a plate in open air, when you see green on the plate squirt a syringe full of iso at any percent on it and suck it up, then squirt it in a grain jar and see for yourself just how magic it actually isnt




If it's ineffective why do people use it...do you use it?



I'm not saying it's ineffective it's just not a huge crutch , it's a disinfectant for molds and bacteria but not super effective on spores, I see people daily using iso in their sab  I stead of soap and water or they use Lysol or bleach but you can be just as effective if not more with soap and water and not have to work in a fire hazard, jars and shit can get wiped down because you don't want to saturate your filters and iso evaporates quickly which is also why it's not great for sab prep




bod said the other day that he doesn't even use soapy water anymore to prep his SAB... he said he leaves the walls dry with nothing.  Maybe you guys can debate?  I am just interested in learning.



there isn't much to debate, he also does unmodded tubs where I stick to classic monos but the end goal is the same


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OfflineSockadin
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Re: Significant difference in isopropyl alcohol? [Re: cronicr]
    #26536890 - 03/15/20 03:39 PM (3 years, 10 months ago)

By classic do you mean poly or MP tape?


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InvisiblebodhisattaMDiscordReddit
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Re: Significant difference in isopropyl alcohol? [Re: Sockadin]
    #26536891 - 03/15/20 03:40 PM (3 years, 10 months ago)

Holes in general


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Invisibletramalot
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Re: Significant difference in isopropyl alcohol? [Re: bodhisatta]
    #26536991 - 03/15/20 04:45 PM (3 years, 10 months ago)

Most SAB cleaning can be done with just soap & bleach or Lysol ECT.

Save ISO for scaple\loop hands and jars. A qt should last 100-200 jars bags or plates easy


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Invisiblegizmo1
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Re: Significant difference in isopropyl alcohol? [Re: tramalot]
    #26537313 - 03/15/20 07:40 PM (3 years, 10 months ago)

How many oz of distilled water do I need to add to 16oz of 91% to get it to around 70%?
Is there a formula I can use to figure % I suck at math.
If I mix 50% and 70% 50/50 what percentage will I get.

Can I get a crash course in figuring percentages?


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