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Offlinemuchlove
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Hyphal knots or contamination?
    #26533525 - 03/13/20 09:22 PM (3 years, 10 months ago)



hey so I have what looks to be a ton of knots all over my sub but im coming here to see if that is indeed what it is or something else? looks like knots to me. only problem is this is Penis envy and these knots are coming in really early. only been 12 days since putting into bins to colonize and i was going to case it today but these showed up since the 10th. what should i do? should I case or let it blob and pick the first flush then case after it? or should i toss it I cant tell if this is contamination or not. there is no bad smell. I did open the bins on the 10th to check on them but they werent fully colonized and now they have this all over them.


Edited by muchlove (03/13/20 09:38 PM)


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InvisibleStipe-n CapMDiscord
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Re: Hyphal knots or contamination? [Re: muchlove]
    #26533547 - 03/13/20 09:44 PM (3 years, 10 months ago)

Looks like a metric fuck tonne of trich.


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InvisiblefahtsterM
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Re: Hyphal knots or contamination? [Re: muchlove]
    #26533548 - 03/13/20 09:45 PM (3 years, 10 months ago)

Looks like trich before it sporelates.. could be myc but I doubt it.. you’ll know either way in a day or two.  Starts to turn greenish, toss it immediately away from your grow room... outside is best

Faht


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Offlinemuchlove
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Re: Hyphal knots or contamination? [Re: Stipe-n Cap]
    #26533590 - 03/13/20 10:15 PM (3 years, 10 months ago)

yeah prob is trich. i feel like i see some green in some places but not 100% sure. This contamination shit has really been kicking my ass. Ive been trying to get a clean liquid culture for some months. I feel like it might be some other factor though, I didnt clean the room down too well from a previous grow I had that got trich and was colonizing this bin in the same room. I find it difficult to believe that the culture is contaminated because I tested it on agar using a drop from the same syringe I used to inoculate all the jars with and nothing showed up on the agar, and all the jars looked good with a good smell. looked clean. if you dont thoroughly clean a room that had a bin in it with trich can it contaminate future bins? I thought that mycelium has a pretty good defense barrier if it colonizes the substrate well enough.


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Offlinejcm4620
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Re: Hyphal knots or contamination? [Re: muchlove]
    #26533610 - 03/13/20 10:26 PM (3 years, 10 months ago)

dump that thing fast holy shit that is a metric ton of trich wow


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OfflineTheDuckMan
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Re: Hyphal knots or contamination? [Re: jcm4620]
    #26533637 - 03/13/20 10:49 PM (3 years, 10 months ago)

So the white fuzz balls are the trich?


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Offlinemuchlove
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Re: Hyphal knots or contamination? [Re: TheDuckMan]
    #26533705 - 03/13/20 11:50 PM (3 years, 10 months ago)





okay well im 99% sure this is a serious case of trich, I've never had this much trich occur at once. almost positive it cant be knots because its only been 12 days since jars were put into the bin and it just doesnt look right. what do you guys think? I'm assuming its from the room that it was in because there were trich contaminated bins in the room about a week before I moved this in and I didnt clean the room before moving these in there. no sign whatsoever of trich was showing in my spawn jars before using, the spawn jars were shaken at about 30% colonization too and nothing showed, they were perfectly white and smelled good. a drop of the LC from the same syringe used to inoculate the jars was put on agar which grew out perfectly with no signs of contamination. The bin after 10 days looked fine and was about 90% done colonizing the substrate and then boom, it exploded into this in 2 days. Might have also been the coir, I only used the bhod cooler pseudo pasteurize/sterilize method. might just pressure cook it next time for 90 mins.

how should I go about cleaning the room after this? Lysol the whole room and wipe things down with rubbing alcohol? I have a small hepa air filter in there to circulate air but it clearly didnt help. I noticed there was a small piece (about an inch square) of dried substrate that I had cut out of that old contaminated bin that was a trich contam'd piece I cut out to finish flushing the old substrate, maybe the dried spores were carried into the rest of the bins during colonization by the air filter circulating air in the room.


Edited by muchlove (03/13/20 11:59 PM)


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