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OfflineAtmozFear
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Finally found an SAB setup and routine that suits me! * 2
    #26526692 - 03/10/20 06:38 AM (3 years, 11 months ago)

three stacked lids that gliiiiide across the bottom of the box, coupled with fly paper placed on the top lid- the combo meaning adjustable arm positions, while the fly paper keeps the jars from slipping or sliding.  My elbows sit on a scrap marble block.  I spooned out the half-liquified agar with culture on it, holding the spoon to an alcohol lamp between scoops and cooling with an immovable alcohol-soaked paper towel wedged under the marble.

I think tonight I will try to use an unopened syringe to suck up some other agar I have, colonized and half-liquid... and try injecting some jars without even using the SAB.  btw why in the hell do I not hear about a thousand other people trying this?  seems like a no-brainer.


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Edited by AtmozFear (03/10/20 06:41 AM)


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OfflineLogicaL ChaosM
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26526795 - 03/10/20 08:03 AM (3 years, 11 months ago)

Very cool. I like yoir creative design.

I wouldnt recommend openning an agar culture outside a SAB but if you wish to gamble :shrug:


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InvisibleLadysKnight
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Re: Finally found an SAB setup and routine that suits me! [Re: LogicaL Chaos]
    #26526797 - 03/10/20 08:07 AM (3 years, 11 months ago)

"cooling with an immovable alcohol-soaked paper towel wedged under the marble"

Everything coming out nice and clean?


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: LogicaL Chaos] * 1
    #26527371 - 03/10/20 01:49 PM (3 years, 11 months ago)

Quote:

LogicaL Chaos said:
Very cool. I like yoir creative design.

I wouldnt recommend openning an agar culture outside a SAB but if you wish to gamble :shrug:




No no, that jar on the side was just an empty jar to set my spoon in as I am getting ready... I am opening the agar inside the SAB. 

Thanks for the compliment!  I sat around during my PC cycle, just brain storming and going over what exactly I was going to do inside the SAB.


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: LadysKnight]
    #26527380 - 03/10/20 01:52 PM (3 years, 11 months ago)

Quote:

LadysKnight said:
"cooling with an immovable alcohol-soaked paper towel wedged under the marble"

Everything coming out nice and clean?



Sorry about the bump- wanted to reply to everyone...
yes, it seems very simple and fundamental, but I always had a stupid little problem of dragging or sliding the alcohol soaked paper towel whenever I tried to cool after the flame.


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OfflineLogicaL ChaosM
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26527442 - 03/10/20 02:09 PM (3 years, 11 months ago)

Ah i see. I must have misread your post or somethang. Whoops!


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: LogicaL Chaos] * 1
    #26527452 - 03/10/20 02:13 PM (3 years, 11 months ago)

:thumbup: no problemo


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OfflineOne of Us
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26527561 - 03/10/20 03:11 PM (3 years, 11 months ago)

Quote:

AtmozFear said:
Quote:

LadysKnight said:
"cooling with an immovable alcohol-soaked paper towel wedged under the marble"

Everything coming out nice and clean?



Sorry about the bump- wanted to reply to everyone...
yes, it seems very simple and fundamental, but I always had a stupid little problem of dragging or sliding the alcohol soaked paper towel whenever I tried to cool after the flame.




I think what LadysKnight was getting at here is that cooling something with alcohol soaked paper towel is not generally seen as a wise move. The alcohol will get rid of the bacteria, but not any mold spores that may be on the paper towel.  These spores can end up sticking to whatever you cool effectively contaminating whatever you just flame sterilized.

A lot of people are skipping the syringe/spoon and just pouring into the receiving jar or whatever (one less contam vector).  Media bottles are great for liquid cultures/inoculates because of their no-drip ring/lip.


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InvisibleInocuole
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Re: Finally found an SAB setup and routine that suits me! [Re: One of Us]
    #26527791 - 03/10/20 05:17 PM (3 years, 11 months ago)

You're literally better off putting the searing hot blade right into the agar than to try to do anything to "cool" it down.  Also, why not have the SAB upside down so you have a proper flat surface?


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InvisiblemushboyMDiscord
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Re: Finally found an SAB setup and routine that suits me! [Re: Inocuole] * 1
    #26527842 - 03/10/20 05:40 PM (3 years, 11 months ago)

:whathesaid:

Not to mention any disturbance caused by coming in contact with the tote. Think the game operation:stoned:


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: mushboy]
    #26527851 - 03/10/20 05:48 PM (3 years, 11 months ago)

Quote:

mushboy said:
:whathesaid:

Not to mention any disturbance caused by coming in contact with the tote. Think the game operation:stoned:



Well it is a wood surface under the tote, not a very smooth surface, so 1) all kinds of microbeasties in the wood and 2)  the whole point of the lids being inside the tote is that they slide around very easily, whereas they would have too much friction on the wood.

Quote:

Inocuole said:
You're literally better off putting the searing hot blade right into the agar than to try to do anything to "cool" it down.



Awesome, did not know that.  I thought the heat could kill some mycellium.


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Edited by AtmozFear (03/10/20 05:51 PM)


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InvisibleInocuole
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear] * 1
    #26527874 - 03/10/20 05:55 PM (3 years, 11 months ago)

It can kill whatever mycelium it first touches but a little dead mycelium is better than a little living mold.

Regarding the wood surface, have you considered using a damp towel or like, maybe a thin rubber mat as the floor of the SAB?  Problem is if you touch the bottom and it's connected to the walls, you get vibrations/disturbances to the whole thing, and that can knock little particles loose or create tiny air currents.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: Inocuole]
    #26527911 - 03/10/20 06:07 PM (3 years, 11 months ago)

Quote:

Inocuole said:
It can kill whatever mycelium it first touches but a little dead mycelium is better than a little living mold.

Regarding the wood surface, have you considered using a damp towel or like, maybe a thin rubber mat as the floor of the SAB?  Problem is if you touch the bottom and it's connected to the walls, you get vibrations/disturbances to the whole thing, and that can knock little particles loose or create tiny air currents.



It is too much work dealing with you.  I'm not gonna repeat my thread intro.  You are pessimistic as hell and you're only here to criticize because you had a bad day.


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InvisibleInocuole
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26529395 - 03/11/20 04:00 PM (3 years, 11 months ago)

K good luck :cheers:


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: Inocuole]
    #26529405 - 03/11/20 04:04 PM (3 years, 11 months ago)

thanks... very magnanimous of you, I appreciate it!


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InvisibleLadysKnight
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26529446 - 03/11/20 04:36 PM (3 years, 11 months ago)

Easy killer, we wouldn't say anything at all if we didn't give a fuck. I know it's hard, but gotta keep the ego in check for peer review.


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Re: Finally found an SAB setup and routine that suits me! [Re: LadysKnight]
    #26529468 - 03/11/20 04:50 PM (3 years, 11 months ago)

Not trying to ruin your day bro.  I just didn't understand why something easily slidable and able to channel percussive force to the whole box would be an ideal surface to work on.  You don't have to explain if you don't want to but, gotta pick your brain a little bit since you factored in an alcohol soaked paper towel in your design and that's a sign you haven't been at this long. :shrug:

I mean it is what it is, and I'm sorry for my unpolished tone.  I could've dropped the word "literally" and you'd probably have received it better.  But lets be real, you were having a worse day than I was by the sound of it.

Whatever the case, do your thing man. :thumbup:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: Inocuole]
    #26529515 - 03/11/20 05:21 PM (3 years, 11 months ago)

the alcohol soaked paper towel is a problem?  Everywhere I go on here and on YouTube show people using dozens of them... I am not THAT new, been at it for a year, already done more than a few successful bulk grows.  I would really appreciate it if you avoided me around here, very very annoying.

You are nothing compared to Bod, and LogicaL...  looks like you have more experience than me, but I am already better.


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Edited by AtmozFear (03/11/20 05:24 PM)


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OfflineA.k.aM
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear] * 1
    #26529634 - 03/11/20 06:36 PM (3 years, 11 months ago)

The usual way to cool the blade is to stick it into the clean agar of the dish you’re about to transfer to.


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: A.k.a]
    #26529636 - 03/11/20 06:38 PM (3 years, 11 months ago)

that's a good idea.  I was transferring to grain tho.


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