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OfflineNyptonian
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Registered: 03/02/20
Posts: 12
Last seen: 9 months, 19 days
Is this contam?
    #26529269 - 03/11/20 02:37 PM (3 years, 10 months ago)

Hi just started this i'm new to this. Bought a growbag inoculated it I was clean used gloves, iso 70% wiped down everything and flamed the needle. The bag was kept in the dark at 80ish and above for few days read on here that it was safer to keep it in the 70s to keep contams at bay have been doing that since.





This is how the bag looks it's been about 13 days since I inoculated it. A bit concerned about some of the gray in the grains. I did put 5 cc in the bag which I'm guessing is too much for 1lb?

I did all this before reading this forum and the different teks I could use. This forum is great very useful I appreciate all the guys and ladies contributing their info and tek. Planning on going with sff shoebox tek and start from scratch after this. I bought a pressure cooker, rye grains, a SAB and some pint jars. Also going to try my hand at agar since it seems the most fool proof way to Inoculate the grains.

Thanks for any feedback

posted this in the contam section but no reply


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Is this contam? [Re: Nyptonian]
    #26529298 - 03/11/20 02:55 PM (3 years, 10 months ago)

Whomever made that bag made it to wet.
Did you inoculate with a spore syringe?
If it was a spore syringe, it should go to agar first to prevent contaminations.
I would trash that bag then inoculate plates of agar with your syringe.


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OfflineFrankRhizo
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Re: Is this contam? [Re: Strainsfordaze]
    #26529314 - 03/11/20 03:04 PM (3 years, 10 months ago)

Yes contaminated.
Yes too much spore solution.
No agar isn't fool proof but it will greatly increase your chances.
Keep your chin up, work with agar, learn what a clean culture looks like and you will have success.


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Invisiblerickyswamps
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Registered: 11/08/18
Posts: 1,192
Re: Is this contam? [Re: FrankRhizo]
    #26529379 - 03/11/20 03:47 PM (3 years, 10 months ago)

Thats got mold and bacteria.  Looks like it wasn't even sterilized or the syringe was completely garbage.  Sorry, but you can toss it out.  What you said is the right path, there are a lot of people here to learn from and lots of good teks for spores>agar>grains>spawn to shoebox or tub.


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OfflineNyptonian
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Registered: 03/02/20
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Re: Is this contam? [Re: rickyswamps]
    #26529572 - 03/11/20 05:56 PM (3 years, 10 months ago)

Thanks for the help guys gonna throw away the bag and start agar work. I'm thinking about using Pastywhyte's Easy Agar Tek is that a good starting point?


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Is this contam? [Re: Nyptonian]
    #26529638 - 03/11/20 06:39 PM (3 years, 10 months ago)

Yes.


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OfflineC12ShroomMan
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Registered: 01/19/20
Posts: 239
Last seen: 3 years, 3 months
Re: Is this contam? [Re: Nyptonian]
    #26529648 - 03/11/20 06:43 PM (3 years, 10 months ago)

Quote:

Nyptonian said:
Thanks for the help guys gonna throw away the bag and start agar work. I'm thinking about using Pastywhyte's Easy Agar Tek is that a good starting point?




if you buy a small pack of petri dishes to start and pour them in a SAB it will be easier to see whats growing.


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Offlinejbgtaa
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Registered: 06/09/19
Posts: 1,785
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Re: Is this contam? [Re: C12ShroomMan]
    #26529654 - 03/11/20 06:45 PM (3 years, 10 months ago)

I’d start with real petris. Not only are they easier to see what’s inside and to use but also pouring them clean and successfully will teach you good sterile technique


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