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OfflineZombie-ant
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Agar mycelium take 2
    #26529162 - 03/11/20 01:21 PM (3 years, 10 months ago)

The yellow circle is trich and the our Le circle is bacteria right?
The rest look fine?


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Offlinefootpath
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Re: Agar mycelium take 2 [Re: Zombie-ant]
    #26529174 - 03/11/20 01:30 PM (3 years, 10 months ago)

The yellow is probably penicillium or aspergillus - a mold, nonetheless. The blue is a bacteria.

I wouldn't say the rest look fine. You let them grow out too far and it's gonna be hell to try to take a workable transfer. But I'd probably go for something in that 4-6 o'clock range on the fourth image there.


Edited by footpath (03/11/20 01:33 PM)


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OfflineMagic Mushpoon
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Re: Agar mycelium take 2 [Re: footpath]
    #26529182 - 03/11/20 01:35 PM (3 years, 10 months ago)

Trich is green. These plates look rough. What's your sterile procedure?


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OfflineMadMyc
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Re: Agar mycelium take 2 [Re: Zombie-ant]
    #26529185 - 03/11/20 01:36 PM (3 years, 10 months ago)

Nothing there looks like cube mycelium to me.


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Offlinefootpath
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Re: Agar mycelium take 2 [Re: Magic Mushpoon]
    #26529213 - 03/11/20 01:54 PM (3 years, 10 months ago)

He circled things with designated colors. A yellow circle. A blue circle.
Trichoderma turns green, aspergillus turns green, penicilium turns green - the latter two are far more common.

edit: read wrong. My b, madmyc. However, I do think there's some cube myc there.


Edited by footpath (03/11/20 01:55 PM)


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OfflineZombie-ant
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Re: Agar mycelium take 2 [Re: footpath]
    #26529224 - 03/11/20 02:05 PM (3 years, 10 months ago)

Quote:

footpath said:
He circled things with designated colors. A yellow circle. A blue circle.
Trichoderma turns green, aspergillus turns green, penicilium turns green - the latter two are far more common.

edit: read wrong. My b, madmyc. However, I do think there's some cube myc there.





Is it best practice to cut out myc that surrounds spore inoculation areas?


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OfflineMagic Mushpoon
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Re: Agar mycelium take 2 [Re: Zombie-ant]
    #26529231 - 03/11/20 02:10 PM (3 years, 10 months ago)

You want to take a small (grain of rice sized) sample from the leading edge of mycelium. Not trying to be a dick here, but you should really read some of the agar resources on here. It doesn't really make sense to do agar before you know how to do agar.
https://www.shroomery.org/forums/showflat.php/Number/21922023
This is a good place to start.


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Offlinefootpath
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Re: Agar mycelium take 2 [Re: Zombie-ant]
    #26529239 - 03/11/20 02:16 PM (3 years, 10 months ago)

Quote:

Zombie-ant said:
Quote:

footpath said:
He circled things with designated colors. A yellow circle. A blue circle.
Trichoderma turns green, aspergillus turns green, penicilium turns green - the latter two are far more common.

edit: read wrong. My b, madmyc. However, I do think there's some cube myc there.





Is it best practice to cut out myc that surrounds spore inoculation areas?



Yeah. Not super early, but definitely before it fills out the plate.
If I were you, I would probably just start a new plate and, if you feel like experimenting, grab a transfer from this plate,

Like I said, probably around that 4-5 o'clock area. See how you can kind of make out a thick dot that radiates outward? Some of that outward growth may get you something you can work with. Doubtful, but maybe. It'll be a good learning experience either way.
Otherwise, just start a new plate and show us before it grows out too much.


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OfflineZombie-ant
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Re: Agar mycelium take 2 [Re: footpath]
    #26529290 - 03/11/20 02:51 PM (3 years, 10 months ago)

Quote:

footpath said:
Quote:

Zombie-ant said:
Quote:

footpath said:
He circled things with designated colors. A yellow circle. A blue circle.
Trichoderma turns green, aspergillus turns green, penicilium turns green - the latter two are far more common.

edit: read wrong. My b, madmyc. However, I do think there's some cube myc there.





Is it best practice to cut out myc that surrounds spore inoculation areas?



Yeah. Not super early, but definitely before it fills out the plate.
If I were you, I would probably just start a new plate and, if you feel like experimenting, grab a transfer from this plate,

Like I said, probably around that 4-5 o'clock area. See how you can kind of make out a thick dot that radiates outward? Some of that outward growth may get you something you can work with. Doubtful, but maybe. It'll be a good learning experience either way.
Otherwise, just start a new plate and show us before it grows out too much.




Cut out these triangular sectors?


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Offlinefootpath
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Re: Agar mycelium take 2 [Re: Zombie-ant]
    #26529403 - 03/11/20 04:04 PM (3 years, 10 months ago)

Not the whole thing, just a little bit (grain of rice sized) of some of that outer growth. I'd go for the one closer to 4 o'clock.


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OfflineShquonk
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Re: Agar mycelium take 2 [Re: footpath]
    #26529459 - 03/11/20 04:43 PM (3 years, 10 months ago)

Do NOT try and just cut out the contamination. You gotta move a healthy piece of myc to a new dish. Trying to cut out contam is usually futile because its probably all over the dish already especially if the mold is sporulating in the dish. Trash it and start with a new healthy piece of myc that you can salvage and keep transfering until its fully clean. Also when you cut out a clean piece of myc make sure the contam is away from you so you dont bring it over the contaminated spot and ruin your next dish.

Oops I read a reply wrong but its still good information nonetheless


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Edited by Shquonk (03/11/20 04:45 PM)


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OfflineZombie-ant
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Re: Agar mycelium take 2 [Re: Shquonk]
    #26529494 - 03/11/20 05:09 PM (3 years, 10 months ago)

Is it worth cleaning up Myc using H2O2


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OfflineShquonk
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Re: Agar mycelium take 2 [Re: Zombie-ant]
    #26529510 - 03/11/20 05:18 PM (3 years, 10 months ago)

No


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OfflineSockadin
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Re: Agar mycelium take 2 [Re: Zombie-ant]
    #26529665 - 03/11/20 06:51 PM (3 years, 10 months ago)

Quote:

Zombie-ant said:
The yellow circle is trich and the our Le circle is bacteria right?
The rest look fine?
[/quote
Can we talk about your dates. Does everyone in the UK do day month year?


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OfflineMagic Mushpoon
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Re: Agar mycelium take 2 [Re: Sockadin]
    #26529880 - 03/11/20 08:43 PM (3 years, 10 months ago)

All you need to do if you want to get a transfer (which at this point is risky) is take a small sample from the leading edge of mycelium from on the colonies where the spore germinated. Don't take the whole thing. No need for H2O2. (Never heard of that being used for agar work). Your goal is not to cut out the contams, its to isolate healthy growth.


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OfflineZombie-ant
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Re: Agar mycelium take 2 [Re: Magic Mushpoon]
    #26530155 - 03/12/20 01:00 AM (3 years, 10 months ago)

Before anyone says do some reading... I have: bods, kizzle and other TCs. I’m still none the wiser at recognising early rhizomorphic growth. Bacteria I think I get (literally and metaphorically), green/yellow slimy stuff, like ectoplasm from Slimer (ghostbusters).

fluffy stuff mold...

Maybe I’m getting old and need specs


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OfflineZombie-ant
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Re: Agar mycelium take 2 [Re: Zombie-ant]
    #26530747 - 03/12/20 10:50 AM (3 years, 10 months ago)

Here are close ups in what I think are mycelium... an I wrong



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Edited by Zombie-ant (03/12/20 10:55 AM)


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OfflineShquonk
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Re: Agar mycelium take 2 [Re: Zombie-ant]
    #26530772 - 03/12/20 11:02 AM (3 years, 10 months ago)

Go for it. Youll probably have to keep cleaning it up but that's a good place to start


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