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OfflineEbilPhish
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Registered: 06/20/18
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ID Request: Melbourne (collected last year) * 1
    #26528468 - 03/11/20 03:55 AM (3 years, 10 months ago)

These two where actually from last year, late in the sub season.

They where just 2 small ones, way past their prime and kind of gnarly so I forget about them. Since then I've decided it would be nice to try and get some growing in the garden and I still have the spore print. Unfortunately the prints have been sitting out in the open for a while but hopefully I can clean them up on agar. Failing that maybe I can find some this upcoming season.

Collected from woodchip areas.

They weren't very big. About fat thumb sized. They do have purplish brown spore print. But I can't tell if the darkness on the stems was bruising or just them rotting.



Edited by EbilPhish (03/11/20 04:29 AM)


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OfflineAnglerfishM
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Re: ID Request: Melbourne [Re: EbilPhish]
    #26528473 - 03/11/20 04:03 AM (3 years, 10 months ago)

Looks like waterlogged P. subaeruginosa.  Perhaps you should add "from last year" in the headline of your post, to avoid confusion.


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Offlineobtuse
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Re: ID Request: Melbourne [Re: Anglerfish]
    #26528513 - 03/11/20 05:08 AM (3 years, 10 months ago)

I agree that they are water logged Psilocybe subaeruginosa

Can see blue bruising on the gills and the stem of the righthand specimen.  the spore print looks good too.

if you want to transfer to agar, prints need to be super clean, and if you have had them in the open then other spores and bacteria could be a problem.  if you use the dilution tek it could work for you when you plate out. or use a small amount of spores and do good long streaks across the agar.


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OfflineEbilPhish
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Re: ID Request: Melbourne [Re: obtuse]
    #26528552 - 03/11/20 06:11 AM (3 years, 10 months ago)

Quote:

Anglerfish said:
Looks like waterlogged P. subaeruginosa.  Perhaps you should add "from last year" in the headline of your post, to avoid confusion.




Added :smile:

Quote:

obtuse said:
I agree that they are water logged Psilocybe subaeruginosa

Can see blue bruising on the gills and the stem of the righthand specimen.  the spore print looks good too.

if you want to transfer to agar, prints need to be super clean, and if you have had them in the open then other spores and bacteria could be a problem.  if you use the dilution tek it could work for you when you plate out. or use a small amount of spores and do good long streaks across the agar.




They have probably be sitting out for like 6 months in a dusty house, so I'm sure contams will be a problem.

I was planning to follow c10's agar streaking. And maybe a hotpour with hydrogen peroxide agar.

Got a link to the the dilution tek though? Not finding a whole lot in the search.

I'm hoping with them being woodlovers they will be more contam resistant and the goal is an outdoor grow so full sterile isn't an issue in the long run but that's assuming I can bootstrap some mycelium from the spores. Might try putting an agar wedge on a woodchip or maybe even try scraping some spores directly on a chip and keeping it moist.


Edited by EbilPhish (03/11/20 06:56 AM)


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Offlineobtuse
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Re: ID Request: Melbourne [Re: EbilPhish]
    #26529497 - 03/11/20 05:12 PM (3 years, 10 months ago)

Hydrogen peroxide kills spores.  its the major reason why its such a useful method.  but you cannot use it for spore prints.

dilution:  take say half or quarter of the print and wash spores into 1 ml of water, then add this to 9 ml of water.  you can repeat as many times as you want, i.e take 1 ml of that solution add to a further 9 ml.

the idea being is that when you plate it out there is less concentration of spores and hopefully far less concentration of contaminating spores.

someone sent me a print that had been sitting out in the open for a week, i had nothing but trouble across 20 plates, so i gave up on that print.


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