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InvisibleLadysKnight
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Registered: 10/09/15
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Defeating mold on agar
    #26527177 - 03/10/20 12:06 PM (3 years, 10 months ago)

I have a couple dirty prints that I'd like to grow out, but the mold germinates and colonizes the plate before the mush. It's the sexy clear halo type. No matter when or where I take transfers, the mold comes with it.

I occasionally use black tea agar to freeze bacteria in place to allow myc to outgrow it. Does anyone have a method for defeating enmeshed mold on agar?


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OfflinecronicrFacebook
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Re: Defeating mold on agar [Re: LadysKnight]
    #26527206 - 03/10/20 12:27 PM (3 years, 10 months ago)

mold spores on prints you are gonna be hard pressed to find any secret.
swipe an s pattern, transfer quick:shrug:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineShquonk
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Registered: 01/02/19
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Re: Defeating mold on agar [Re: LadysKnight]
    #26527210 - 03/10/20 12:29 PM (3 years, 10 months ago)

Sorry but it sounds to me like you need a new culture or spores.


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Ain't that just the way.


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OfflineTGS
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Re: Defeating mold on agar [Re: Shquonk]
    #26527486 - 03/10/20 02:32 PM (3 years, 10 months ago)

Curious for a general approach to this problem of clear/fine mold intertwined with what we want.

Keep cutting and isolating in hopes of outrunning it. How long before you give up, 10 transfers, 20?

Run a tub of MS from syringe and try to catch a print/clone before it greens out.

Drop syringe on agar and start over?  Seems to germinate better on grain than agar.


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InvisibleLadysKnight
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Re: Defeating mold on agar [Re: TGS]
    #26528023 - 03/10/20 07:15 PM (3 years, 10 months ago)

Quote:

cronicr said:
mold spores on prints you are gonna be hard pressed to find any secret.
swipe an s pattern, transfer quick:shrug:




Thanks, at least I know it's futile.

Quote:

Shquonk said:
Sorry but it sounds to me like you need a new culture or spores.




Yep, about to give it up.
Quote:

TGS said:
Curious for a general approach to this problem of clear/fine mold intertwined with what we want.

Keep cutting and isolating in hopes of outrunning it. How long before you give up, 10 transfers, 20?

Run a tub of MS from syringe and try to catch a print/clone before it greens out.

Drop syringe on agar and start over?  Seems to germinate better on grain than agar.




I've been working on this print for 5 months, definitely done with trying from spores. Have about a dozen brf pucks, a few Muda bottles and 6 shoeboxes going with hopes to squeak out a clone. But no expectations.

Thanks all.


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OfflineMycobolical
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Re: Defeating mold on agar [Re: LadysKnight]
    #26528251 - 03/10/20 09:57 PM (3 years, 10 months ago)

I've had similar problems. It could very well have been a fluke, but I managed to transfer away when let the plates colonize at a lower temp. Around 65-70°F. Like I said could just be a fluke but maybe worth a shot.


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Invisiblewoofwoof
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Registered: 01/04/19
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Re: Defeating mold on agar [Re: Mycobolical]
    #26528264 - 03/10/20 10:07 PM (3 years, 10 months ago)

try Josex biopsy method


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InvisibleLadysKnight
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Re: Defeating mold on agar [Re: woofwoof]
    #26528785 - 03/11/20 09:07 AM (3 years, 10 months ago)

Quote:

Mycobolical said:
I've had similar problems. It could very well have been a fluke, but I managed to transfer away when let the plates colonize at a lower temp. Around 65-70°F. Like I said could just be a fluke but maybe worth a shot.




I've tried waiting until the mush myc overtakes the mold, which occasionally happens, then transferring the tinyest scrape of the very tip of the most outer edge. Fails every time. Those are my temps also.

Quote:

woofwoof said:
try Josex biopsy method




I tried this with my brf pucks. They look good on brf, but when I transfer back to agar, there it is.


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