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OfflineSandala
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Registered: 02/20/20
Posts: 197
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Last seen: 1 year, 9 months
spores on agar, isolate or?
    #26526996 - 03/10/20 10:10 AM (3 years, 10 months ago)

Hey all,

Complete noob here, first shroom shinanigans...

this is day 7, cube spores on PDA agar. (I have 10 dishes all together but they all look pretty similar)



plan is to inoculate oat grain in jars, is it worth trying to isolate any of these tufts to new dishes before they grow together, or just let it all colonize the plate and cut chunks for the jars? or isolate some bits when it's grown a bit more, or something else :laugh:

This one is kinda wierd, it was a bunch of spores stuck together in the syringe that dint break up with shaking, contam? or can a spore clump grow out like this?

Day 5


Day 7


same blob diff background


Cheers, San:rasta:


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Offlinejcm4620
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Registered: 05/26/19
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Re: spores on agar, isolate or? [Re: Sandala]
    #26527060 - 03/10/20 10:55 AM (3 years, 10 months ago)

garbage the ones with those brown spots yuck take transfers from other and c what happens


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PANAEOLUS FRUITING MADE SIMPLE



Edited by jcm4620 (03/10/20 10:56 AM)


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OfflineHamHead
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Registered: 03/17/15
Posts: 6,107
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Re: spores on agar, isolate or? [Re: jcm4620]
    #26527102 - 03/10/20 11:23 AM (3 years, 10 months ago)

I'll usually wait until there's about a dime sized growth and start transfers from there.

Don't worry about isolates, just transfer until you get some clean cultures and start dropping to grains. Isolates takes many transfers to get a true isolate.


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The Italian researchers’ findings, published by the INT’s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February.

https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF

This online first version has been peer-reviewed, accepted and edited,  but not formatted and finalized with corrections from authors and proofreaders

https://www.icandecide.org/


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OfflineSandala
Noob Shroomer

Registered: 02/20/20
Posts: 197
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Last seen: 1 year, 9 months
Re: spores on agar, isolate or? [Re: HamHead]
    #26527342 - 03/10/20 01:37 PM (3 years, 10 months ago)

thanks chaps

if the plates have no contam is it even worth transfering before inoculating grain?


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OfflineNeowynd8
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Registered: 06/12/16
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Re: spores on agar, isolate or? [Re: Sandala]
    #26527737 - 03/10/20 04:54 PM (3 years, 10 months ago)

Most would say transfer a few times even if you don't see anything. You could do both. Take a transfer and use the rest to inoculate if you think it's clean


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InvisibleInfiniteDreams
Male

Registered: 10/25/19
Posts: 1,224
Re: spores on agar, isolate or? [Re: Neowynd8]
    #26527748 - 03/10/20 05:04 PM (3 years, 10 months ago)

Quote:

Neowynd8 said:
Most would say transfer a few times even if you don't see anything. You could do both. Take a transfer and use the rest to inoculate if you think it's clean




:whathesaid:

Why limit yourself?


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OfflineSandala
Noob Shroomer

Registered: 02/20/20
Posts: 197
Loc: UK Flag
Last seen: 1 year, 9 months
Re: spores on agar, isolate or? [Re: Neowynd8]
    #26527913 - 03/10/20 06:08 PM (3 years, 10 months ago)

Quote:

Neowynd8 said:
Most would say transfer a few times even if you don't see anything. You could do both. Take a transfer and use the rest to inoculate if you think it's clean




Excellent, thanks. I could do the next batch from 2nd or 3rd transfers, sounds like a good plan, I'll do that :thumbup:


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OfflineSandala
Noob Shroomer

Registered: 02/20/20
Posts: 197
Loc: UK Flag
Last seen: 1 year, 9 months
Re: spores on agar, isolate or? [Re: Sandala]
    #26546487 - 03/20/20 11:44 AM (3 years, 10 months ago)

OK so I let them grow out a bit more, can now see signs of rhyzomorphic growth (is that even the right word :lol:

Am I best trying to take transfers from those rhyzomorphic areas?



Not sure about these BHT spores, the spores seem to have taken well in the mycobox/growkit...



...but don't seem to like agar, or is it contam? is it even worth trying to transfer anything from this plate?



San:rasta:


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