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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Tell me about your Lab [Re: LotKid]
    #26553728 - 03/23/20 06:48 PM (3 years, 10 months ago)

Nice flow cabinet


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InvisibleLotKid
Never.Trust.A.Prankster
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Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
Trusted Cultivator
Re: Tell me about your Lab [Re: Strainsfordaze] * 1
    #26553741 - 03/23/20 06:53 PM (3 years, 10 months ago)

Thanks. I love that thing


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


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OfflineBirdSeedUndaUrFeet
Micropope
I'm a teapot User Gallery


Registered: 10/29/18
Posts: 248
Loc: Germany
Last seen: 3 years, 4 months
Re: Tell me about your Lab [Re: LotKid] * 3
    #26553997 - 03/23/20 09:00 PM (3 years, 10 months ago)

PCs.



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OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
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Folding@home Statistics
Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible... Flag
Last seen: 2 hours, 13 minutes
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Re: Tell me about your Lab [Re: LotKid]
    #26558522 - 03/26/20 07:31 AM (3 years, 9 months ago)

Damn dude, that is one serious lab u got there! :eek: :mindblown:


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Offlinemonkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 23 days
Re: Tell me about your Lab [Re: gizmo1]
    #26558585 - 03/26/20 08:24 AM (3 years, 9 months ago)

Quote:

gizmo1 said:
Most definitely. Thank you contams suck but going through a severe outbreak can make or break a cultivator. If you dont let it break you then you can learn alot from it.




This... been there lost like 4 tubs back to back with 6q in each and just gave up for awhile. Back at it now though trying to flex my agar muscle.


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: Tell me about your Lab [Re: LogicaL Chaos] * 2
    #26558588 - 03/26/20 08:27 AM (3 years, 9 months ago)

Upgraded to a flowhood happy camper





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Offlinemonkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 23 days
Re: Tell me about your Lab [Re: Infinity-25]
    #26558593 - 03/26/20 08:31 AM (3 years, 9 months ago)

Quote:

Infinity-25 said:






It never crossed my mind to get more than one pyrex pouring bottle until i saw this photo...:bow2:


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Offlinemonkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 23 days
Re: Tell me about your Lab [Re: D3_Myc] * 2
    #26558600 - 03/26/20 08:34 AM (3 years, 9 months ago)

Quote:

D3monic said:
Is it a terrible idea that my lab and grow are the same space? Just opposite sides of the room. I’m in the process of building a flowhood right now. Should be done with final coats of polyurethaning tomorrow






Rocking chair for maximum shrooming capabilities.  :rockon:


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InvisibleLotKid
Never.Trust.A.Prankster
Male User Gallery

Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
Trusted Cultivator
Re: Tell me about your Lab [Re: monkey_accident]
    #26558606 - 03/26/20 08:38 AM (3 years, 9 months ago)



--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


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Offlinemonkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 23 days
Re: Tell me about your Lab [Re: LotKid]
    #26558613 - 03/26/20 08:42 AM (3 years, 9 months ago)

Niiiiice. Little foil over the top and careful handling do the trick with these? I see myself getting boiling agar all over the place without a lid..


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InvisibleLotKid
Never.Trust.A.Prankster
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Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: Tell me about your Lab [Re: monkey_accident]
    #26558620 - 03/26/20 08:46 AM (3 years, 9 months ago)

Yup, foil over the top. I use the 2 liter beaker to prep 1 liter of agar and that gets me 20 plates. 20 plates easliy lasts me a month or 2. I prep in my aa915 and open it to remove the sterilzed beaker in front of my filter.


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


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InvisibleInfiniteDreams
Male

Registered: 10/25/19
Posts: 1,224
Re: Tell me about your Lab [Re: LotKid] * 1
    #26558639 - 03/26/20 08:57 AM (3 years, 9 months ago)

You pour your plates pretty thick.  800mL gets me about 40 plates (100mm size)


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Offlinemonkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 23 days
Re: Tell me about your Lab [Re: InfiniteDreams] * 1
    #26558653 - 03/26/20 09:06 AM (3 years, 9 months ago)

Yeah I got the same ratio, 400mL gets me 20 plates.


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Tell me about your Lab [Re: LotKid] * 1
    #26558670 - 03/26/20 09:15 AM (3 years, 9 months ago)

I thought I poured thick, 1L give me 40 plates(100mm)
What kind of dishes are you using?


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InvisibleLotKid
Never.Trust.A.Prankster
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Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
Trusted Cultivator
Re: Tell me about your Lab [Re: Strainsfordaze] * 1
    #26558737 - 03/26/20 09:40 AM (3 years, 9 months ago)

Maybe it is 40 plates and i'm just retarded. We cant rule that out...


Edit: I need to pour some more plates anyway... so we'll see if i'm just brain fartin'... which i'm kinda thinking i might be.


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


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Offlinemonkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 23 days
Re: Tell me about your Lab [Re: LotKid]
    #26558820 - 03/26/20 10:12 AM (3 years, 9 months ago)

Quote:

LotKid said:
Yup, foil over the top. I use the 2 liter beaker to prep 1 liter of agar and that gets me 20 plates. 20 plates easliy lasts me a month or 2. I prep in my aa915 and open it to remove the sterilzed beaker in front of my filter.




Also 20 plates for a month? I'm just getting comfortable with agar but I feel like you'd use more if you have multiple vars ur trying to isolate/different isolates of the same var. I just ordered a case of 500... should last me a while


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InvisibleLotKid
Never.Trust.A.Prankster
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Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
Trusted Cultivator
Re: Tell me about your Lab [Re: monkey_accident]
    #26558829 - 03/26/20 10:15 AM (3 years, 9 months ago)

Fuck tryig to isolate. Mostly a just a rabbit hole.


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


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Offlinemonkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 4 months, 23 days
Re: Tell me about your Lab [Re: LotKid]
    #26558844 - 03/26/20 10:20 AM (3 years, 9 months ago)

Yeah if we're talking a monoculture true isolate, I'm right there with you. But generally a few transfers for less competition of genetics should give quicker colonization times if memory serves me correctly.
edit: as well as a more consistent fruiting


Edited by monkey_accident (03/26/20 10:21 AM)


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 4 hours, 5 minutes
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Re: Tell me about your Lab [Re: monkey_accident]
    #26558864 - 03/26/20 10:32 AM (3 years, 9 months ago)

I was really surprised the difference a transfer or two can make.

These are the same clone but I think t3 and t1.



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LAGM2020


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InvisibleInfiniteDreams
Male

Registered: 10/25/19
Posts: 1,224
Re: Tell me about your Lab [Re: monkey_accident]
    #26558877 - 03/26/20 10:36 AM (3 years, 9 months ago)

I went through a ton of agar at first because:
  • I needed practice and to learn
  • I needed backup in case a had contam problems
  • I didn't really know how things would work


In the end I've thrown out plenty of awesome plates and ended up only being beneficial for learning the skills.

I see once you know your process as long as you've got good technique there's no need to burn through many plates.

And if you clone you are close enough to an "isolate" anyway.

But back on topic, LotKid, that's a sweet lab.


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