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Shquonk
Prof. Mushroom Grower


Registered: 01/02/19
Posts: 127
Last seen: 2 years, 6 months
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Re: Tell me about your Lab [Re: A.k.a]
#26530328 - 03/12/20 06:04 AM (3 years, 10 months ago) |
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You can do any other kind of sterile work such as g2g and spore Inoculation.
-------------------- Ain't that just the way.
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: Tell me about your Lab [Re: Shquonk]
#26530338 - 03/12/20 06:18 AM (3 years, 10 months ago) |
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When I first started I bleached my entire bathroom top to bottom bleached the toilet and the shower and the sink drains, bombed the room with Lysol and ISO and washed my whole body in antibacterial soap just to knock up some brf jars... burnt the shit out of my lungs too.
Once i started working inside a SAB on the kitchen table things got much easier and my contamination rate in Petris was pretty low like 1 in 50 poured plates got contaminated...
SABS are the shit
I’m lucky I didn’t pass out from using ISO and bleach in the same room. I guess when you mix ISO and bleach it creates chloroform gas
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Tell me about your Lab [Re: footpath]
#26530358 - 03/12/20 06:53 AM (3 years, 10 months ago) |
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Quote:
footpath said: Definitely the larger ones you see at gas stations and hardware stores - they'll be 20lb propane tanks. You can get a lot out of them... I'd say at least 20 2hr PC cycles. You hardly need any flame at all to your PC to 15-18PSI
Presto's are aluminum, BTW. So, just keep it on the lower end. Don't let the flames get anywhere close to curling up around the base and you'll be good.
 Not the best depiction, but you can see that it's pretty damn low.
So I'm pretty late to this thread, but I moved my PC to one of these years ago before retirement. It is worth every penny. I can run one PC on it and the other on my Cadco hot plate and get 16qts done at the same time.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 54 minutes
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Re: Tell me about your Lab [Re: Sockadin] 1
#26530429 - 03/12/20 07:52 AM (3 years, 10 months ago) |
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I love hearing that, my burner comes tomorrow I’m so ready.
Lol spunky it’s so funny how mega paranoid we all were at first. I would do literally almost nothing to get ready to knock up brf jars at this point.
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LAGM2020     
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Tell me about your Lab [Re: A.k.a]
#26530894 - 03/12/20 12:08 PM (3 years, 10 months ago) |
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I will say that my Cadco hot plate gets up to venting way faster than the propane burner. I think the open flame is less efficient, but works to boil grains.
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: Tell me about your Lab [Re: A.k.a]
#26531022 - 03/12/20 01:18 PM (3 years, 10 months ago) |
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Quote:
A.k.a said: I love hearing that, my burner comes tomorrow I’m so ready.
Lol spunky it’s so funny how mega paranoid we all were at first. I would do literally almost nothing to get ready to knock up brf jars at this point.
Exactally after I got started on grains I knocked up some brf in open air with no flame just an ISO wipe between jars and they all still produced.
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SpearCaps
On a journey


Registered: 11/18/19
Posts: 110
Last seen: 3 years, 9 months
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Re: Tell me about your Lab [Re: A.k.a]
#26534843 - 03/14/20 01:21 PM (3 years, 10 months ago) |
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Quote:
A.k.a said: The quick turn around is what makes this so much better than plants.
Once you get the basics down it’s like an addicting game, trying to always do better or discover the perfect clone.
My “lab” is just an empty bedroom with a table for the SAB, and a drawer desk thing next to it I store my plates and agar stuff in.
Besides that it’s just tubs everywhere.
Thats my dream.
Unfortunately i have no bedroom to spare. I don'T have place for monos, so there is no point for me to go into Grain atm (as my PC is tiny also).
But in the future i will have such a spare room and it will be epic!
-------------------- Outside of right and wrong is a place. There we'll meet. - Sufi Wisdom
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Tell me about your Lab [Re: SpearCaps] 2
#26534848 - 03/14/20 01:25 PM (3 years, 10 months ago) |
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All you need is a closet and the same bedroom you sleep in. My first pressure cooker was able to do either 2 or 3 quart jars at a time I don't remember for sure but I still used to get it in. I had fun doing it too. All I had was the closet in my bedroom to fruit in and the dresser in my bedroom to do sterile work on. I've been through many different situations and I always made it work.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 54 minutes
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Re: Tell me about your Lab [Re: gizmo1]
#26534868 - 03/14/20 01:33 PM (3 years, 10 months ago) |
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Yeah it’s such a flexible hobby it’s great. No permanent set ups really.
Up until today I’ve only been able to pc one quart at a time, but when that quart turns to two ounces of mushrooms it’s pretty sweet.
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LAGM2020     
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Kimble
Idiot

Registered: 03/08/18
Posts: 508
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Re: Tell me about your Lab [Re: A.k.a]
#26538927 - 03/16/20 03:53 PM (3 years, 10 months ago) |
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My apartment kitchen is my lab when I need it to be.
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SpearCaps
On a journey


Registered: 11/18/19
Posts: 110
Last seen: 3 years, 9 months
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Re: Tell me about your Lab [Re: Kimble]
#26541143 - 03/17/20 04:36 PM (3 years, 10 months ago) |
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Guys i stocked up and got a massive 140l Box for a new SAB. It will be much easier to work with alot of Jars then and in all more comfortable.
@Kimble your SAB looks too good to be true. what is your contam rate?
Quote:
gizmo1 said: All you need is a closet and the same bedroom you sleep in. My first pressure cooker was able to do either 2 or 3 quart jars at a time I don't remember for sure but I still used to get it in. I had fun doing it too. All I had was the closet in my bedroom to fruit in and the dresser in my bedroom to do sterile work on. I've been through many different situations and I always made it work.
What if you don't have a closet? Actually how did u fruit in your closet? I'd imagine the FAE and GE would have been terrible. You used modded or unmodded FCs?
-------------------- Outside of right and wrong is a place. There we'll meet. - Sufi Wisdom
Edited by SpearCaps (03/17/20 04:37 PM)
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LongBeak


Registered: 11/15/19
Posts: 43
Last seen: 3 years, 8 months
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Re: Tell me about your Lab [Re: SpearCaps]
#26541301 - 03/17/20 06:22 PM (3 years, 10 months ago) |
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My lab is stuffed in a closet and I take the pieces out and set them up whenever I need to work. Basically just a Presto 23qt PC, small SAB (54qts), tub of oats, torch, spray bottles (iso and water).
The only other thing is that I have a cabinet with "weather stripping" and a raspberry Pi controlled heat lamp that I use to incubate some jars, LCs and plates. Also I keep the food dehydrator out because it looks nice enough in the kitchen.
Basically everything set up perfectly to grow in a small apt and not bother my wife with the clutter.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Tell me about your Lab [Re: SpearCaps]
#26541806 - 03/17/20 11:03 PM (3 years, 10 months ago) |
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Quote:
SpearCaps said: Guys i stocked up and got a massive 140l Box for a new SAB. It will be much easier to work with alot of Jars then and in all more comfortable.
@Kimble your SAB looks too good to be true. what is your contam rate?
Quote:
gizmo1 said: All you need is a closet and the same bedroom you sleep in. My first pressure cooker was able to do either 2 or 3 quart jars at a time I don't remember for sure but I still used to get it in. I had fun doing it too. All I had was the closet in my bedroom to fruit in and the dresser in my bedroom to do sterile work on. I've been through many different situations and I always made it work.
What if you don't have a closet? Actually how did u fruit in your closet? I'd imagine the FAE and GE would have been terrible. You used modded or unmodded FCs?
Horrible gas exchange in a closet? No. FAE no problem I run modified monos and unmodified shoeboxes. Never had a problem I also left the door open most of the time though. I still fruit in a closet. Check out the OS mini mono link in my signature all the tubs in the bottom were fruited in a closet. No fan running or anything like that. Amount of FAE is controlled by how tight you stuff the holes but I've never had to loosen them I usually just stuff them as tight as possible. I'll be honest FAE could be a little better but closet is stealthier. It's a trade off. Also who doesn't have a closet nowadays what are you living under a rock lmao. Just kidding bro
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Tell me about your Lab [Re: gizmo1]
#26541829 - 03/17/20 11:40 PM (3 years, 10 months ago) |
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Is it a terrible idea that my lab and grow are the same space? Just opposite sides of the room. I’m in the process of building a flowhood right now. Should be done with final coats of polyurethaning tomorrow
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Tell me about your Lab [Re: D3_Myc] 1
#26541887 - 03/18/20 12:33 AM (3 years, 10 months ago) |
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Quote:
D3monic said: Is it a terrible idea that my lab and grow are the same space? Just opposite sides of the room. I’m in the process of building a flowhood right now. Should be done with final coats of polyurethaning tomorrow

Na shouldn't be a problem. You can definitely still do sterile work where you fruit even if it's not ideal. In a ideal world you wouldn't want anything else going on in your sterile lab but... I mean we are(most of us) amateur mycologist and hobbyists. We do what we have to to get the job done. Most of us don't have separate rooms to dedicate to growing. It's all good.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 7 hours
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Re: Tell me about your Lab [Re: gizmo1]
#26541912 - 03/18/20 01:07 AM (3 years, 10 months ago) |
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 54 minutes
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Re: Tell me about your Lab [Re: D3_Myc]
#26542018 - 03/18/20 05:03 AM (3 years, 10 months ago) |
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Quote:
D3monic said: Is it a terrible idea that my lab and grow are the same space? Just opposite sides of the room. I’m in the process of building a flowhood right now. Should be done with final coats of polyurethaning tomorrow

Hasn’t made a difference for me lol.
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LAGM2020     
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: Tell me about your Lab [Re: A.k.a]
#26542092 - 03/18/20 06:38 AM (3 years, 10 months ago) |
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Quote:
A.k.a said:
Quote:
D3monic said: Is it a terrible idea that my lab and grow are the same space? Just opposite sides of the room. I’m in the process of building a flowhood right now. Should be done with final coats of polyurethaning tomorrow

Hasn’t made a difference for me lol.

You actually got shoeboxes on top of your SAB, lol
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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The extra weight keeps the SaB in place.
Here are mine on the shelf above my FH.
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: Tell me about your Lab [Re: Sockadin] 4
#26553714 - 03/23/20 06:40 PM (3 years, 10 months ago) |
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Sorry its a lil messy but, i work in there.
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