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OfflineAtmozFear
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Finally found an SAB setup and routine that suits me! * 2
    #26526692 - 03/10/20 06:38 AM (3 years, 10 months ago)

three stacked lids that gliiiiide across the bottom of the box, coupled with fly paper placed on the top lid- the combo meaning adjustable arm positions, while the fly paper keeps the jars from slipping or sliding.  My elbows sit on a scrap marble block.  I spooned out the half-liquified agar with culture on it, holding the spoon to an alcohol lamp between scoops and cooling with an immovable alcohol-soaked paper towel wedged under the marble.

I think tonight I will try to use an unopened syringe to suck up some other agar I have, colonized and half-liquid... and try injecting some jars without even using the SAB.  btw why in the hell do I not hear about a thousand other people trying this?  seems like a no-brainer.


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Edited by AtmozFear (03/10/20 06:41 AM)


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OfflineLogicaL ChaosM
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26526795 - 03/10/20 08:03 AM (3 years, 10 months ago)

Very cool. I like yoir creative design.

I wouldnt recommend openning an agar culture outside a SAB but if you wish to gamble :shrug:


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InvisibleLadysKnight
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Re: Finally found an SAB setup and routine that suits me! [Re: LogicaL Chaos]
    #26526797 - 03/10/20 08:07 AM (3 years, 10 months ago)

"cooling with an immovable alcohol-soaked paper towel wedged under the marble"

Everything coming out nice and clean?


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: LogicaL Chaos] * 1
    #26527371 - 03/10/20 01:49 PM (3 years, 10 months ago)

Quote:

LogicaL Chaos said:
Very cool. I like yoir creative design.

I wouldnt recommend openning an agar culture outside a SAB but if you wish to gamble :shrug:




No no, that jar on the side was just an empty jar to set my spoon in as I am getting ready... I am opening the agar inside the SAB. 

Thanks for the compliment!  I sat around during my PC cycle, just brain storming and going over what exactly I was going to do inside the SAB.


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: LadysKnight]
    #26527380 - 03/10/20 01:52 PM (3 years, 10 months ago)

Quote:

LadysKnight said:
"cooling with an immovable alcohol-soaked paper towel wedged under the marble"

Everything coming out nice and clean?



Sorry about the bump- wanted to reply to everyone...
yes, it seems very simple and fundamental, but I always had a stupid little problem of dragging or sliding the alcohol soaked paper towel whenever I tried to cool after the flame.


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OfflineLogicaL ChaosM
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26527442 - 03/10/20 02:09 PM (3 years, 10 months ago)

Ah i see. I must have misread your post or somethang. Whoops!


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: LogicaL Chaos] * 1
    #26527452 - 03/10/20 02:13 PM (3 years, 10 months ago)

:thumbup: no problemo


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OfflineOne of Us
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26527561 - 03/10/20 03:11 PM (3 years, 10 months ago)

Quote:

AtmozFear said:
Quote:

LadysKnight said:
"cooling with an immovable alcohol-soaked paper towel wedged under the marble"

Everything coming out nice and clean?



Sorry about the bump- wanted to reply to everyone...
yes, it seems very simple and fundamental, but I always had a stupid little problem of dragging or sliding the alcohol soaked paper towel whenever I tried to cool after the flame.




I think what LadysKnight was getting at here is that cooling something with alcohol soaked paper towel is not generally seen as a wise move. The alcohol will get rid of the bacteria, but not any mold spores that may be on the paper towel.  These spores can end up sticking to whatever you cool effectively contaminating whatever you just flame sterilized.

A lot of people are skipping the syringe/spoon and just pouring into the receiving jar or whatever (one less contam vector).  Media bottles are great for liquid cultures/inoculates because of their no-drip ring/lip.


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InvisibleInocuole
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Re: Finally found an SAB setup and routine that suits me! [Re: One of Us]
    #26527791 - 03/10/20 05:17 PM (3 years, 10 months ago)

You're literally better off putting the searing hot blade right into the agar than to try to do anything to "cool" it down.  Also, why not have the SAB upside down so you have a proper flat surface?


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Re: Finally found an SAB setup and routine that suits me! [Re: Inocuole] * 1
    #26527842 - 03/10/20 05:40 PM (3 years, 10 months ago)

:whathesaid:

Not to mention any disturbance caused by coming in contact with the tote. Think the game operation:stoned:


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: mushboy]
    #26527851 - 03/10/20 05:48 PM (3 years, 10 months ago)

Quote:

mushboy said:
:whathesaid:

Not to mention any disturbance caused by coming in contact with the tote. Think the game operation:stoned:



Well it is a wood surface under the tote, not a very smooth surface, so 1) all kinds of microbeasties in the wood and 2)  the whole point of the lids being inside the tote is that they slide around very easily, whereas they would have too much friction on the wood.

Quote:

Inocuole said:
You're literally better off putting the searing hot blade right into the agar than to try to do anything to "cool" it down.



Awesome, did not know that.  I thought the heat could kill some mycellium.


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Edited by AtmozFear (03/10/20 05:51 PM)


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InvisibleInocuole
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear] * 1
    #26527874 - 03/10/20 05:55 PM (3 years, 10 months ago)

It can kill whatever mycelium it first touches but a little dead mycelium is better than a little living mold.

Regarding the wood surface, have you considered using a damp towel or like, maybe a thin rubber mat as the floor of the SAB?  Problem is if you touch the bottom and it's connected to the walls, you get vibrations/disturbances to the whole thing, and that can knock little particles loose or create tiny air currents.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: Inocuole]
    #26527911 - 03/10/20 06:07 PM (3 years, 10 months ago)

Quote:

Inocuole said:
It can kill whatever mycelium it first touches but a little dead mycelium is better than a little living mold.

Regarding the wood surface, have you considered using a damp towel or like, maybe a thin rubber mat as the floor of the SAB?  Problem is if you touch the bottom and it's connected to the walls, you get vibrations/disturbances to the whole thing, and that can knock little particles loose or create tiny air currents.



It is too much work dealing with you.  I'm not gonna repeat my thread intro.  You are pessimistic as hell and you're only here to criticize because you had a bad day.


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InvisibleInocuole
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26529395 - 03/11/20 04:00 PM (3 years, 10 months ago)

K good luck :cheers:


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: Inocuole]
    #26529405 - 03/11/20 04:04 PM (3 years, 10 months ago)

thanks... very magnanimous of you, I appreciate it!


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InvisibleLadysKnight
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26529446 - 03/11/20 04:36 PM (3 years, 10 months ago)

Easy killer, we wouldn't say anything at all if we didn't give a fuck. I know it's hard, but gotta keep the ego in check for peer review.


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Re: Finally found an SAB setup and routine that suits me! [Re: LadysKnight]
    #26529468 - 03/11/20 04:50 PM (3 years, 10 months ago)

Not trying to ruin your day bro.  I just didn't understand why something easily slidable and able to channel percussive force to the whole box would be an ideal surface to work on.  You don't have to explain if you don't want to but, gotta pick your brain a little bit since you factored in an alcohol soaked paper towel in your design and that's a sign you haven't been at this long. :shrug:

I mean it is what it is, and I'm sorry for my unpolished tone.  I could've dropped the word "literally" and you'd probably have received it better.  But lets be real, you were having a worse day than I was by the sound of it.

Whatever the case, do your thing man. :thumbup:


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: Inocuole]
    #26529515 - 03/11/20 05:21 PM (3 years, 10 months ago)

the alcohol soaked paper towel is a problem?  Everywhere I go on here and on YouTube show people using dozens of them... I am not THAT new, been at it for a year, already done more than a few successful bulk grows.  I would really appreciate it if you avoided me around here, very very annoying.

You are nothing compared to Bod, and LogicaL...  looks like you have more experience than me, but I am already better.


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Edited by AtmozFear (03/11/20 05:24 PM)


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OfflineA.k.aM
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear] * 1
    #26529634 - 03/11/20 06:36 PM (3 years, 10 months ago)

The usual way to cool the blade is to stick it into the clean agar of the dish you’re about to transfer to.


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OfflineAtmozFear
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Re: Finally found an SAB setup and routine that suits me! [Re: A.k.a]
    #26529636 - 03/11/20 06:38 PM (3 years, 10 months ago)

that's a good idea.  I was transferring to grain tho.


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OfflineRandalf the Grey
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear] * 1
    #26530891 - 03/12/20 12:07 PM (3 years, 10 months ago)

Definitely frowned upon to touch anything that is not sterile with a utensil after it has been flamed. You are taking it from sterile to sanitary, which is a backwards step.

Also, as a new guys, I can say there is FOR SURE a lot of bad information and misinformation out there. This place is great because while there is old info, there are also people here to correct that old info and point you in the right direction of the up-to-date info.

I like the idea of arm hole covers to prevent stuff floating into your SAB, but do the swing close each time? Seems that may be pushing some outside air into SAB. Maybe remove during work and replace for storage to prevent as much air movement as possible. When I first started I decided to use some painters plastic and a PVC frame to build a positive pressure room to do my sterile work.
I planned it, I built it, I used it, and I quickly realized I did not understand some of the finer details (eddies inside the room fucked EVERYTHING up) and the reason no one else was really doing it was because it was far easier, faster, cheaper, and more effective to just use the same KISS style SAB that 90% of people use. There is a reason why so many people were using them; they work.

If the surface of your table is a issue, have you tried a rack to raise everything up. I know some people go without, but when starting out it is a nice bit of a safety net. Dulls the impact of your movements but also saves your lids and tools from touching the "floor" if dropped. Also makes some work easier by being able to have, for example, your plate sitting higher up than your grain jar.
I get it man, i really do. I am a tinkerer and constantly invent or reinvent things in my mind. I am not trying to take a shot at you or knock you down.
It seems like there are several people here trying to offer genuine advice but are being received as attacks. Yes, there are some assholes in this forum, but even assholes has good advice sometimes.
I'm not saying you must follow what the masses are doing (clearly, we are not that kind of people or we wouldn't be here) and it is always ok to listen to advice without acting on it. "It is the mark of an educated mind to entertain and idea with accepting it as truth". Aristotle never said this but a lot of people claim he did and its message is true.

as for cooling your spoon, the easiest and safest way is to dip in agar of either a clean plate, or the edge of the plate you are taking culture from. Even if you don't, similar to flaming the needle of an MSS, the very first myc(spores) that touch the hot metal will die, and everything after that will be fine and sterile. If you must use a ISO towel, I would at the very least keep in the SAB, not open air. The whole time it is sitting there it is subjected to potential contams both from the air, and every single time you reach your arms over it to do work.

You are thinking and that is never a bad thing, but focus on your technique more than you equipment. Mushboy is a bit of a minimalist I believe and advocates dry SAB, no towels, no lid, and I think no rack if I remember correctly. (not trying to speak for mushboy, just have read several of his discussions on the topic). The only thing you need to make this work is Still Air, and good technique. Everything else is just a bonus or a safety net.
I would definitely not recommend opening any plate in open air. You might get lucky, you might not. I do all my work in a Still Air Room with no vents or windows or anything to cause currents but the simple act of me walking in and moving around brings and stirs up contams.

If your agar is so soft that you need a spoon to work it, instead of a blade, I would suggest a measuring spoon (tblsp/tsp/etc). Less surface area means less to flame and less chance of contams settling on it.

Truly not trying to attack you or your ideas here. I have had those eureka moments that turn out to be not eureka moments in practice. I assure you, there is better advice here than on youtube or reddit or FB (if that group still exists).


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OfflineBrian Jones
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Re: Finally found an SAB setup and routine that suits me! [Re: AtmozFear]
    #26547440 - 03/20/20 07:29 PM (3 years, 10 months ago)

IMO Inocuole gives good advice frequently around here.


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Re: Finally found an SAB setup and routine that suits me! [Re: Brian Jones]
    #26547720 - 03/20/20 10:02 PM (3 years, 10 months ago)

What a great read. Suggestion instead of attaching hole covers to your SAB tape some tyvek sleeves to your gloves then when you enter the SAB you have a bunch of tyvek blocking air movements in the hole while you work.
YouTube: anything you see of YouTube is a copy of teks that where created here. This is why so much of the creation and innovation of this site and the members is interesting.

I recently watched the Gordo Tek vid and most of it was pretty spot on except I disagree with his grain prep, (I think that is the next big area to focus on)

My point is that, if your doing things that work for you. Fuck everyone else's opinion. Most people just reiterate the same shit they have read and take it as doctrine.

  Times change, people evolve their thinking. Now we have unmoded tubs and no pours. And that God Damn Humidity Chamber. ( Not a fan) But I have not tried it yet and even though I have an opinion about it, I can't really argue against it...


Edited by Sockadin (03/20/20 10:12 PM)


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InvisibleInocuole
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Re: Finally found an SAB setup and routine that suits me! [Re: Brian Jones]
    #26549889 - 03/21/20 10:13 PM (3 years, 10 months ago)

Quote:

Brian Jones said:
IMO Inocuole gives good advice frequently around here.



Eh we're all over it, let's not bring it up needlessly. <3

Thanks though.

If anybody's still worried about an argument on a forum with the other shit we got going on, their priorities are fucked.  Now is the time to be nice to everybody.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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