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OnlyLoseMeLegs
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Rye grain contam or just wet? + Help me grow my first shroom!
#26526459 - 03/10/20 12:42 AM (3 years, 10 months ago) |
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Hey guys, it's my first time growing and unfortunately, due to circumstances I had to buy pre sterilized grain bags. I recived 2 today and on the bottom of both bags there are swollen and some cracked grains that look discolored. I did some searching and saw some posts with similar looking grains with people saying they are just too wet but I'm not sure. What do you think?
Edited by OnlyLoseMeLegs (04/15/20 04:19 PM)
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BooShow
Spooky



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That looks like wet-spot contamination, unfortunately. It happens when the grain isn't properly sterilized. It'll have a ripe, dirty socks kinda smell. Here's a pic of wet spot contamination:
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OnlyLoseMeLegs
Stranger Danger
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Re: Rye grain contam or just wet? [Re: BooShow]
#26526485 - 03/10/20 01:23 AM (3 years, 10 months ago) |
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That's a real bummer, was really excited to start
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BooShow
Spooky



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Quote:
OnlyLoseMeLegs said: That's a real bummer, was really excited to start 
Yeah, that sucks man. Especially buying them pre-sterilized. You should see if you can get your money back or at least another batch of grain. Send them those pics too.
Your best bet is to buy some jars, buy some grain, get your hands on a PC, and do it yourself. Trust me, man. Grains are simple AF once you do them a couple of times.
Don't get too discouraged bro; we all get contams. It comes with the territory. Keep at it and you'll have cubies in no time.
 
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OnlyLoseMeLegs
Stranger Danger
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Re: Rye grain contam or just wet? [Re: BooShow]
#26526556 - 03/10/20 03:35 AM (3 years, 10 months ago) |
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Just got a reply from them, I sent some pics and this is what they had to say,
"its not uncommon for some grains on the bottom to get some extra excess water and normal" "if your worried sit the bag somewhere dark and cool for a week and if nothing grows they are fine"
They also say they've been making them for 10+ years, they work in a class 100 lab and everything is 100% sterile when they leave the factory.
Sounds a bit sketchy to me. Any thoughts?
Also definitely not stopping till I get this right And I'll be getting a PC as soon as I can.
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BooShow
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All I know is anytime my grains looked like that, they were always contaminated.
IMO, it's Bacillus subtilis.

And it's not so much about their lab being 'sterile', they didn't properly sterilize the grain, i.e. proper pressure cooking/autoclaving. The fact that they won't own up to their mistake is rather sketchy. Where did you get the bags from?
However, I wouldn't toss them just yet. I agree that you should let them sit for a couple days or so and see if it spreads. It's definitely wetspot but sometimes you can get away with it (if the bacteria isn't too strong) and you're using it for bulk.
This is a quote from Trusted Cultivator TheEaglesGift: The reason bacillus is a large issue is because often times it'll cause jars to stall and never even finish colonization, or grain to never recover once you use it for spawn(if the bacteria was serious enough). Bacteria isn't a common or major contamination of bulk substrates, so you're fine to use those jars for spawn, just don't go using any jars with bacteria as g2g masters.
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Edited by BooShow (03/10/20 04:12 AM)
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OnlyLoseMeLegs
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Re: Rye grain contam or just wet? [Re: BooShow]
#26526588 - 03/10/20 04:21 AM (3 years, 10 months ago) |
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I got them from aussimushroomsupplies, pretty much the only place other then reddit to find bags where i live. They have multiple different websites too.
Knew it would be risky before I ordered but I gave it a try anyway.
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BooShow
Spooky



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You could always knock 'em up and see what happens lol
-------------------- You are what is. That's all.
  
Edited by BooShow (03/10/20 04:23 AM)
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OnlyLoseMeLegs
Stranger Danger
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Re: Rye grain contam or just wet? [Re: BooShow]
#26526602 - 03/10/20 04:34 AM (3 years, 10 months ago) |
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I have to at least try Haha I'll try keep the thread updated
Edited by OnlyLoseMeLegs (03/20/20 04:22 AM)
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BooShow
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-------------------- You are what is. That's all.
  
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OnlyLoseMeLegs
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Inoculated both bags and am getting signs of growth on first bag but, is it the right kind of growth? It's right where the wet grains are.
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OnlyLoseMeLegs
Stranger Danger
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Getting bigger
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rumfor69
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Yeah let it keep going it might just colonize all the way and work out
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jbgtaa
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Re: Rye grain contam or just wet? [Re: rumfor69]
#26554266 - 03/24/20 12:45 AM (3 years, 10 months ago) |
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If those are syringe-inoculated then you probably don’t have any serious problems to deal with. It looks like semi-solid cubensis mycelium to me. Word to the wise, next time spend your money on a pressure cooker, grains, and jars. This hobby is extremely difficult to do successfully without the proper equipment.
If you can’t afford any of the proper tools look into the PF tek.
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OnlyLoseMeLegs
Stranger Danger
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Re: Rye grain contam or just wet? [Re: jbgtaa]
#26554275 - 03/24/20 12:53 AM (3 years, 10 months ago) |
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PC and equipment just arrived today!
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OnlyLoseMeLegs
Stranger Danger

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More progress, any signs of contam? Should colonization be faster? These are beefy 2kg bags.
Bag 1

bag 2
Edited by OnlyLoseMeLegs (03/30/20 08:27 PM)
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Smartattack
C'mon man



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I think people have it in their heads that moisture = bacteria. In reality bacteria = bacteria. If all signs of moisture in a container of grain meant contamination it would mean that every liquid culture was 100% bacteria. I've had plenty of jars with excess water, some got gross sure, but often they are just wetter. 🤷
Obviously if bacteria finds its way to that water, it has a field day. As long as it's non bacteria containing water - all good.
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OnlyLoseMeLegs
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Hey So, both bags are close to 100% colonisation but there are some bad signs.
Both bags have a couple small yellow (I think) droplets on the side of the bag. metabolites maybe?

The bottom of bag 2 I think looks funky too.

Bag 2 6 days after shaking

Bag 1 4 days from shaking

I'm thinking of spawning into shoeboxes just too see what happens.
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rumfor69
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I bet you're good to go there they finished up nicely. Even if it does have a little bacteria in there it shouldn't affect much.
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Myc097
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Looks good maybe some bacteria, mostly just looks like grains pressed against the bag!
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OnlyLoseMeLegs
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Re: Rye grain contam or just wet? [Re: Myc097]
#26603366 - 04/15/20 04:11 PM (3 years, 9 months ago) |
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Hey guys, thanks for the answers.
I spawned 1:1 spawn/coco/verm to 2 6qt shoeboxes and one 15L tub 3 days ago and there's a lot of condensation with some water pooling. I lined one of the shoeboxes and there's only condensation on the lid but the others have it everywhere. Temps are around 74f to 77f.
15L tub

Shoebox

What can I do?
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CocaineBuffet
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Did you mist it with water? If not mist it with water and put it into fruiting conditions.
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OnlyLoseMeLegs
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Didn't mist, just left them in my closet for the past 3 days. Any harm done putting in fruiting conditions before 100%?
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rumfor69
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No harm, it's ok to go right into fruiting conditions as soon as mixed up.
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OnlyLoseMeLegs
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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26606045 - 04/16/20 03:06 PM (3 years, 9 months ago) |
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So, I put one shoebox and the 15L in fruiting and got my first good look at the substrate surface. The yellow beads are back on the surface of the shoebox and a few on the 15L. (I cased 15L at spawn)
From what I've read I can hopefully get some fruits but it's most likely bacterial and won't produce as many. I guess time will tell.
How's the surface looking anyway? Any bad signs of contam other then the yellow beads?
Edited by OnlyLoseMeLegs (04/16/20 03:07 PM)
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rumfor69
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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26606221 - 04/16/20 05:07 PM (3 years, 9 months ago) |
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I've seen way worse yellow metabolites. Your bacterial contamination doesn't seem to be bad thus far and it just might fruit great. I've had bacterial tubs put out awesome flushes. The surface looks good you don't want yours too overly wet cause of the possible bacteria. If you can maintain it how it looks there with a small amount of misting and fanning a couple times a day you should be good.
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OnlyLoseMeLegs
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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26609090 - 04/17/20 08:43 PM (3 years, 9 months ago) |
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I'm starting to worry about cobweb. Does this look like mycelium? It's not really white and is kinda wispy/cobweby?

Don't wanna be fanning mould everywhere.
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Smartattack
C'mon man



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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26609139 - 04/17/20 09:12 PM (3 years, 9 months ago) |
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It's not cobweb. That shit looks like loose polyfil in your tub.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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rumfor69
Bodhicitta Cultivator



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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26609144 - 04/17/20 09:14 PM (3 years, 9 months ago) |
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Go look at pictures of cobweb mold using the search it should help ease your worries. Some mycelium is just wispy it's called tomentose. But it's still looking ok at this point I think.
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OnlyLoseMeLegs
Stranger Danger

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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26618886 - 04/21/20 10:18 PM (3 years, 9 months ago) |
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Some update photos:
Shoebox 1

Shoebox 2

Tub

Small white dots in every tub/shoebox, sides and bottom too. Are these knots?

Lookin good? Bad?
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rumfor69
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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26618931 - 04/21/20 10:41 PM (3 years, 9 months ago) |
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Those look pretty good and those look like the beginning of knots
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Stage
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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26618944 - 04/21/20 10:48 PM (3 years, 9 months ago) |
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Looks like knots to me.
Shoebox 2 looks sadder than the rest. I don't see knots there. I see a lot of spots of yellow metabolites. This could just be the lighting conditions of the photo though.
I've also seen beads of what looked like very dark metabolites in my tubs, that turned out to just be a bead of clean water acting as a lens on some brown coir. I've taken to shining a little LED at odd coloured beads from different angles to see if it's the juice that's that colour or just what it's resting on.
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OnlyLoseMeLegs
Stranger Danger

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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26652081 - 05/06/20 03:13 AM (3 years, 8 months ago) |
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Yo, Got some pins and fruits from one of the shoeboxes and a bag I spawned to but they're small and don't look healthy. The veils are breaking when they're around 3cm and the stems are dark with streaks. Some of them were pressed up against the bag before I cut it open which deformed some caps.
I put bubble wrap in the shoebox which is why some of these pins are bent.

More fae? Do they look ok to eat?
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fahtster
Now With 33%More Faht


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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: OnlyLoseMeLegs]
#26652085 - 05/06/20 03:19 AM (3 years, 8 months ago) |
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Those are usually signs of being too wet. Give it more FAE . Try it next time without the bubble wrap. They’re fine to eat
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rumfor69
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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: fahtster]
#26652685 - 05/06/20 10:37 AM (3 years, 8 months ago) |
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Hopefully you're into agar and are making a clone or at least some prints to keep things going again
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OnlyLoseMeLegs
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Re: Rye grain contam or just wet? + Help me grow my first shroom! [Re: rumfor69]
#26662939 - 05/10/20 06:29 PM (3 years, 8 months ago) |
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I have everything ready for agar, maybe ill try some cloning on a new tub i have going. Definitely making some prints tho.
Also dried the shroomies, 15g dry so far, in a dehydrator for 12+ hours. They snap in half but they're kinda fluffy/soft inside and don't seem like they'll get any drier. any ideas?
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