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Onlinebio_alchemist
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Registered: 03/21/18
Posts: 590
Loc: Florida
Last seen: 3 minutes, 25 seconds
How I Do Grain Spawn!
    #26525174 - 03/09/20 08:58 AM (3 years, 10 months ago)

I made a video showing how I do Grain Spawn!
I thought this method was worth sharing because it doesn't use the Simmer and evap step that I always found made doing larger quantities of Grain tedious. Check it out and let me know what you guys think!
:super:



--------------------
:heart:
Check out my Odysee Channel!!


Edited by bio_alchemist (03/09/20 08:59 AM)


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InvisibleHobbyist
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Registered: 08/15/10
Posts: 805
Re: How I Do Grain Spawn! [Re: bio_alchemist]
    #26526658 - 03/10/20 06:01 AM (3 years, 10 months ago)

That's a pretty well put together video. :smile: I do have a couple questions though.

When you fold your bag, am I correct in guessing that you fold the filter against the bulk of the bag in the first fold?  That's what it looked like.

Also, you said you let your aa cool overnight, but you show flicking the valve and causing a change in pressure.  You also said you didn't let it pull a vacuum.  Unless I'm missing something pivotal, that looks like a contradiction.

Thanks for sharing


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Everything i say is completely hypothetical...



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Onlinebio_alchemist
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Folding@home Statistics
Registered: 03/21/18
Posts: 590
Loc: Florida
Last seen: 3 minutes, 25 seconds
Re: How I Do Grain Spawn! [Re: Hobbyist]
    #26526877 - 03/10/20 09:06 AM (3 years, 10 months ago)

Thanks!

Quote:

Hobbyist said:
When you fold your bag, am I correct in guessing that you fold the filter against the bulk of the bag in the first fold?





Thats right! Its how I have always done it, but I have barely ever given that any thought, no matter how I fold it the filter always gets covered by a layer of plastic but for whatever reson I decided having it against the bag was better. I guess another difference is that the filter would be face down one way, and face up the other.

Is it better to do it a different way?

Quote:

Hobbyist said:
Unless I'm missing something pivotal, that looks like a contradiction.




I wondered if that part might be confusing, had a hard time deciding where to put that statement. I did in fact let the autoclave depressurize mostly overnight. If you listen close you can hear a rooster crow and morning birds singing before I pop it hah, also no Steam and it very quickly depressurized compared to if you popped the valve right after cycle.

Any criticism greatly appreciated!
Since I have never talked with people about how exactly I do things I expect to hear plenty of ways to improve it!
:cheers:


--------------------
:heart:
Check out my Odysee Channel!!


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InvisibleHobbyist
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Registered: 08/15/10
Posts: 805
Re: How I Do Grain Spawn! [Re: bio_alchemist]
    #26528499 - 03/11/20 04:48 AM (3 years, 10 months ago)

Quote:

bio_alchemist said:

Is it better to do it a different way?





Not at all, I was just curious. :smile:  TBH I don't use bags, but am keenly interested in them.  It's not practical for me to use them right now as I don't have a FH.. :/

Quote:

bio_alchemist said:
Quote:

Hobbyist said:
Unless I'm missing something pivotal, that looks like a contradiction.




I wondered if that part might be confusing, had a hard time deciding where to put that statement. I did in fact let the autoclave depressurize mostly overnight. If you listen close you can hear a rooster crow and morning birds singing before I pop it hah, also no Steam and it very quickly depressurized compared to if you popped the valve right after cycle.

Any criticism greatly appreciated!
Since I have never talked with people about how exactly I do things I expect to hear plenty of ways to improve it!
:cheers:




You're good, once again I was just curious. :smile:

For clarity's sake: it still has positive pressure when you release it?  My AA cools off, reaches atmospheric pressure and starts pulling a vacuum in less than 2 hours from 20psi.


--------------------
Everything i say is completely hypothetical...



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