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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 18 days
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Help me learn how to read agar cultures
#26524300 - 03/08/20 05:15 PM (3 years, 11 months ago) |
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Ok so i successfully made a butt load of plates from MS to get clean mycelium.
Mostly was practicing and making sure i could produce seemingly clean plates. Transfer after transfer etc
Now i have like 3 dozen different plates. When doing transfer 2-4 i tried to pay close attention to sectors. Apparently looking at this particular one there was no isolation at all, right?
Tell me what you see when youre looking at a plate like this. What observations do you make? What is your next move?
Those 2 brownsh specs are gel'd lumps of agar when it cooled too much during the pour.
Edited by shevanel (03/08/20 05:18 PM)
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
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Re: Help me learn how to read agar cultures [Re: shevanel]
#26524344 - 03/08/20 05:56 PM (3 years, 11 months ago) |
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I like 11:00. But yeah I would also out that to grain.
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 18 days
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Re: Help me learn how to read agar cultures [Re: Sockadin]
#26524355 - 03/08/20 06:01 PM (3 years, 11 months ago) |
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why do you like 11? would you transfer that out?
thank you
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mimir
Professional Noob

Registered: 01/21/19
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Loc: Europe
Last seen: 1 month, 1 day
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Re: Help me learn how to read agar cultures [Re: shevanel]
#26524364 - 03/08/20 06:06 PM (3 years, 11 months ago) |
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Quote:
shevanel said: Ok so i successfully made a butt load of plates from MS to get clean mycelium.
Mostly was practicing and making sure i could produce seemingly clean plates. Transfer after transfer etc
Now i have like 3 dozen different plates. When doing transfer 2-4 i tried to pay close attention to sectors. Apparently looking at this particular one there was no isolation at all, right?
Tell me what you see when youre looking at a plate like this. What observations do you make? What is your next move?
Those 2 brownsh specs are gel'd lumps of agar when it cooled too much during the pour.

From what I've read, unless you're trying to create new varieties or isolates you can just go straight to grain/LC as soon as you have a clean plate (yours looks clean to me). My understanding is that isolates need to be tested to see how they perform, which would mean running plenty of different shoeboxes/tubs to see which performs better.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
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Re: Help me learn how to read agar cultures [Re: mimir]
#26524369 - 03/08/20 06:10 PM (3 years, 11 months ago) |
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11:00 is clean and clear growth.
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 18 days
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Re: Help me learn how to read agar cultures [Re: Sockadin]
#26524382 - 03/08/20 06:16 PM (3 years, 11 months ago) |
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@ mimir and sock Ok good info. Thanks.
Soon ill be doing my first cloned tissue to agar. 
Lol to believe i once did it to wbs jars
**march 3rd plates ok here is a plate i shot some GT LC on [right side pic] which was made from a piece of myc on agar. The one on left is multispore Eq plate 1.
On the eq its growing dense vertically and on the edge there is this discolored cresent , probably bacteria? Might be gel'd agar but atop the mound of myc is a couple orange brownish spots, bacteria?
I had it to the side to throw out because nothing came from it [the others all had myc to transfer] so when i found it i decided to keep it and watch what it does when i noticed the rapid growth. 5 days from MS

Edited by shevanel (03/08/20 06:52 PM)
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