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Offlinealphaindigo
Stranger
Registered: 03/07/20
Posts: 1
Last seen: 3 years, 11 months
Messed up liquid culture first time
    #26522510 - 03/07/20 05:02 PM (3 years, 11 months ago)

G’day. This is my first grow, thought I’d be slick and make some LC from a cubensis b plus spore syringe. I did everything right, until I injected 6ccs into my 250 ml jar🥴 I don’t know if I spazzed because it was my first time and I was excited, or I have a really strong thumb...anyway, what’s the bottom line for this? Did I wreck it? What to do? Thank goodness you guys are here and can help!


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OfflineMr.Wizard
Harbinger of Hallucination
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Registered: 01/20/20
Posts: 281
Last seen: 18 days, 7 hours
Re: Messed up liquid culture first time [Re: alphaindigo] * 1
    #26522546 - 03/07/20 05:27 PM (3 years, 11 months ago)

I think it was all going right until you decided to put an MS syringe to LC.

It's not any more wrecked than any MSS going to LC. You just put more of everything from the syringe into your LC.

After you have growth with LC (even when going from clean agar) you should put some to agar to see if it's contaminated.

https://www.shroomery.org/forums/showflat.php/Number/25383024


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OfflineLongBeak
I'm a teapot

Registered: 11/15/19
Posts: 43
Last seen: 3 years, 9 months
Re: Messed up liquid culture first time [Re: alphaindigo]
    #26522569 - 03/07/20 05:36 PM (3 years, 11 months ago)

People generally dont like to go from spores to LC because spore syringes always have contams.

That being said theres a chance it'll work out, especially if it's a good quality spore solution (minimal contams).


Here is what I would do to get from spores to LC: go from spore to fruit. Then go from fruit to LC like so: https://www.shroomery.org/forums/showflat.php/Number/5874305/fpart/all/vc/1



I can give more details if you need.


Edited by LongBeak (03/07/20 05:37 PM)


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