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Invisiblebucket
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Re: Does this substrate look right, at 3 weeks? [Re: Sockadin]
    #26507042 - 02/27/20 06:39 PM (3 years, 11 months ago)

yes. it is just starting to brown.
I have two more tubs mixed, and three more grain bags almost fully colonized.
So still some hope in this go-around. Syringes and bags and substrate were bought online (all prepared products). I will try the quarter-inch drilled tubs.
I am starting to understand the reason for agar transfers, and do-it-yourself grain jars, etc. What I have been thinking was clean growth probably has not been.


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Invisiblebucket
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Re: Does this substrate look right, at 3 weeks? [Re: bucket]
    #26507959 - 02/28/20 10:40 AM (3 years, 11 months ago)

Mycelium or bacteria? This bin is on day 7.
I get the point now, for the complete lab setup, but I am not currently situated for it, so will use up what I have while working on general sanitation, and monotub skills.


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InvisiblefahtsterM
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Re: Does this substrate look right, at 3 weeks? [Re: bucket]
    #26508712 - 02/28/20 06:25 PM (3 years, 11 months ago)

For a week colonizing and the amount of spawn grain in that tub, it should be fully colonized by like day 5.  BUT it could just be slow.. just like last time, I wouldn’t toss yet but it could be bacterial.. if it stalls at that growth, I’d say definitely bacterial.  You May still get fruits with bacterial tubs... depends how bad it is.  Too early to say definitively though.  Is it still colonizing or has it stalled?

Faht


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Invisiblebucket
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Re: Does this substrate look right, at 3 weeks? [Re: fahtster]
    #26509757 - 02/29/20 01:05 PM (3 years, 11 months ago)

It appeared to stall, but I had to leave for two days and will know for sure when I get back.


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Re: Does this substrate look right, at 3 weeks? [Re: PsilocyBen17]
    #26514322 - 03/03/20 09:58 AM (3 years, 11 months ago)

Quote:

PsilocyBen17 said:
:threadmonitor:



Seems like I had dirty syringes in dirty grain bags in dirty substrate all done by a dirty practitioner.
I am going to step back to making brf-verm jars in 8oz. size, steamed on stove-top, inoculated with MS syringe (fingers crossed). I'd still like to spawn to bulk pasteurized coir.
I think that open-air spawning to bulk was my weakest point. Pup tent, fumigated with Lysol???


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OfflineOmbisha
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Re: Does this substrate look right, at 3 weeks? [Re: bucket]
    #26514335 - 03/03/20 10:06 AM (3 years, 11 months ago)

Quote:

I think that open-air spawning to bulk was my weakest point. Pup tent, fumigated with Lysol???




You can spawn to coir in open-air no problem. You also don't need to pasteurize it, you only really need to cook it. I do this by pressure cooking for two hours.


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Invisiblebucket
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Re: Does this substrate look right, at 3 weeks? [Re: Ombisha]
    #26514359 - 03/03/20 10:17 AM (3 years, 11 months ago)

Quote:

Ombisha said:
Quote:

I think that open-air spawning to bulk was my weakest point. Pup tent, fumigated with Lysol???




You can spawn to coir in open-air no problem. You also don't need to pasteurize it, you only really need to cook it. I do this by pressure cooking for two hours.



If I buy Coir in 650g bricks, how does that work, please (pressure cooking it)?


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Re: Does this substrate look right, at 3 weeks? [Re: fahtster]
    #26522351 - 03/07/20 02:51 PM (3 years, 11 months ago)

Quote:

fahtster said:
For a week colonizing and the amount of spawn grain in that tub, it should be fully colonized by like day 5.  BUT it could just be slow.. just like last time, I wouldn’t toss yet but it could be bacterial.. if it stalls at that growth, I’d say definitely bacterial.  You May still get fruits with bacterial tubs... depends how bad it is.  Too early to say definitively though.  Is it still colonizing or has it stalled?

Faht



I had bin #4 set near the back door, ready to go into the garden. Walking by a couple of days later there were 6 gangly looking blessed fruits on one end of the tub (consumed, much as early-birds catch worms), and green patches on the other end. Tubs #5 & #6 are still in play... mixing BRF Cakes, tomorrow. If I get a good specimen from those (or my now doubtful tubs), it will be cloned to agar.


Edited by bucket (03/07/20 03:32 PM)


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Re: Does this substrate look right, at 3 weeks? [Re: bucket]
    #26522356 - 03/07/20 02:58 PM (3 years, 11 months ago)

Why are we still BRF to Bulking? Who ever came up with this ridiculous idea should be flogged.

BRF from MS Syringe is a gamble at best.

Just fruit them as cakes. Way easier to move if they contaminate.


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Invisiblebucket
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Re: Does this substrate look right, at 3 weeks? [Re: Sockadin]
    #26522374 - 03/07/20 03:10 PM (3 years, 11 months ago)

Sockadin said:
Why are we still BRF to Bulking? Whoever came up with this ridiculous idea should be flogged.

BRF from MS Syringe is a gamble at best.

Just fruit them as cakes. Way easier to move if they contaminate.



-I'm not doing BRF to bulk, herein:-)
-MS Syringes are a Be Here Now thing. I don't have anything else to start with:-(
-I hope that this grow-in-jar tek (MS Syringe inoculated) will give me something nice to clone to agar. I will also be doing some MS Syringe to agar at about the same time.


Edited by bucket (03/08/20 07:56 AM)


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