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Armadel
Neub Farmer

Registered: 02/28/20
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white mold on agar
#26521614 - 03/07/20 03:39 AM (3 years, 10 months ago) |
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Hello Shroomery! I'm a newb posting here,not so good at writing specially in forums. anyway, i've been trying to find and buy spores,but i'm living somewhere nobody wants to deal with  so i bought 2g dried golden teacher from a local cultivator.i used dried tissue on pda and got something growing very quickly(first signs in 2-3days) it became horrific dirty with bacteria and molds and i tried to get some clean samples out of it.now i have 3 plates each with 3 inoculation points. now i need help to identify if it's gt myc or white moldy thing :/ sorry for this long write up and thanks in advance for your precious answers

EDIT:more pix may help
Edited by Armadel (03/13/20 02:16 AM)
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Kmacmo
The aborted pin



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Re: white mold on agar [Re: Armadel]
#26521659 - 03/07/20 05:20 AM (3 years, 10 months ago) |
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What part of the shroom did you take a cut from? And was is a large or small shroom?
Plate 3 right hand side transfer looks like it might be the best bet, do you have some better pictures more close up and focused if possible?
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Armadel
Neub Farmer

Registered: 02/28/20
Posts: 22
Last seen: 3 years, 6 months
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Re: white mold on agar [Re: Kmacmo]
#26521689 - 03/07/20 06:02 AM (3 years, 10 months ago) |
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i took small pieces of a medium sized dried gt, from stem,cap and gills in the pictures, you might see 2 small dots in one of transferred samples which are some cracked/powdered parts from gills and let me remind,the pictures are from plates that are cleaned, they contain no piece of mushroom
i will try to get better pictures asap
Edited by Armadel (03/07/20 06:04 AM)
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Kmacmo
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Re: white mold on agar [Re: Armadel]
#26521802 - 03/07/20 08:08 AM (3 years, 10 months ago) |
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I think there good, keep transferring the dense white areas from leading edges while avoiding the transparent 'weak' looking patches. Avoid bumpy while lumps/balls/very cottony/fluffy areas
There's a fair bit of mycelium on your plates just need to keep cleaning
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Armadel
Neub Farmer

Registered: 02/28/20
Posts: 22
Last seen: 3 years, 6 months
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Re: white mold on agar [Re: Kmacmo]
#26521929 - 03/07/20 09:35 AM (3 years, 10 months ago) |
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Oh man! such a good news.i thought i don't even have gt mycelium on my plate and all of them are molds. thank you
Quote:
Kmacmo said: Avoid bumpy while lumps/balls/very cottony/fluffy areas
and what are those actually? can you help me to find them with some example pix? am so excited cuz cultivating golden teacher mushrooms is one of my life goals
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Kmacmo
The aborted pin



Registered: 08/14/19
Posts: 1,675
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Re: white mold on agar [Re: Armadel]
#26522159 - 03/07/20 12:32 PM (3 years, 10 months ago) |
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I've outlined good areas in green and dodgy areas in black. The pink is a good example of mycelium, looks like strands of silk alot of the time youll get these transparent edges on the mycelium.
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Armadel
Neub Farmer

Registered: 02/28/20
Posts: 22
Last seen: 3 years, 6 months
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Re: white mold on agar [Re: Kmacmo]
#26525302 - 03/09/20 10:26 AM (3 years, 10 months ago) |
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thank you so much.i've isolated those areas and preparing 30 new pda plates,i hope it goes well
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Armadel
Neub Farmer

Registered: 02/28/20
Posts: 22
Last seen: 3 years, 6 months
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Re: white mold on agar [Re: Armadel]
#26531999 - 03/13/20 01:42 AM (3 years, 10 months ago) |
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i did 6 transfers and they're growing fine i guess, one thing they have in common is some kinda fluffiness. i'd like to know if it's a good sign,why is it fluffy and why my mycelium doesn't grow like lightning and other mycelium i see in this forum.i'm just worried if i'm growing anything but not golden teacher :/ here's an example of that fluffy stuff
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Kmacmo
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Registered: 08/14/19
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Re: white mold on agar [Re: Armadel]
#26532590 - 03/13/20 11:53 AM (3 years, 10 months ago) |
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Looks okay, the fluff isn't a bad sign just tomentose mycelium that could turn rhizo.
The speed of growth depends of temp, genetics, nutrients etc.. During winter for me I'm usually around 18-20c and it does slow things down but that's alright. Ideally around 25c seems like the sweet spot.
Your newest plates are T2 plates, I'd transfer another once or twice to make sure it's clean
Edited by Kmacmo (03/13/20 12:04 PM)
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Armadel
Neub Farmer

Registered: 02/28/20
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Re: white mold on agar [Re: Kmacmo]
#26535014 - 03/14/20 02:44 PM (3 years, 10 months ago) |
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ok so tomentos and rhizomorphic they're called. thanks.some kinda white contam just appeared in 3 out of 6 plates.i hope the rest will survive. what do you mean by T2?
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Kmacmo
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Registered: 08/14/19
Posts: 1,675
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Re: white mold on agar [Re: Armadel]
#26536046 - 03/15/20 06:41 AM (3 years, 10 months ago) |
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Yeah rhizo is preferred because it will produce fruits like 95% of the time, tomentose won't always produce fruits.
It goes like this: mushroom tissue on plate one is the germ plate.. If you take 3 transfers from that plate they will all be transfer 1 (T1)
Let the T1'S grow enough to transfer then the next plates will be T2 and so on...
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