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Satanic
Stranger



Registered: 10/06/19
Posts: 11
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Colorblind guy tries agar
#26519262 - 03/05/20 06:17 PM (3 years, 11 months ago) |
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Hey guys think I have a clean plate but am colorblind, anyone see anything I can't?
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Colorblind guy tries agar [Re: Satanic]
#26519286 - 03/05/20 06:27 PM (3 years, 11 months ago) |
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It’s white if that’s what you mean.. but it’s not very uniform and doesn’t look to great to be honest.
Most people don’t let plates grow all the way to the edge because they usually get contaminated when they do.
Is this a spore plate? Did you take any transfers?
-------------------- Tek's I use LAGM2020
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Ignorantape
Mycophile


Registered: 01/30/20
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Re: Colorblind guy tries agar [Re: Satanic]
#26519296 - 03/05/20 06:30 PM (3 years, 11 months ago) |
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That's an interesting issue that you'll have to work around.
There seems to be some slight discoloration on the colony that's a 1 o'clock on the plate. I can't see enough detail to say if it's contam or not.
If you're going to take a section then perhaps take it from somewhere else on the plate.
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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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Quote:
staytrippy420 said:Most people don’t let plates grow all the way to the edge because they usually get contaminated when they do.
Curious because I hear this advice often. IME though it has never happened to me personally. I have kept most plates just to watch, and based off this advice assumed they would show a contamination, but it has not occurred. I have had germination plates literally go rhizo when they hit the edge and like ivy grow up the side and along the lid.
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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I’ve seen that as well on some of my neglected plates. Personally I haven’t ever tried to spawn plates that touch the edge. I’m just regurgitating what I’ve read. I have had pasty plates contam when it hits the edge tho.
Im not sure if you would ever see the contam? ... but that doesn’t mean it’s not there.
-------------------- Tek's I use LAGM2020
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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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FWIW I have spawned multiple plates that touched the edge and 0 issue. I've read exactly the same as you, but having not seen it play out in practice, I am questioning it.
Should note I am talking strictly plastic petri dishes, not pastys or any other no pour.
I agree that a contam does not need to be seen that could later cause issue. But the unseen contam that never causes an issue is Schrödinger's contam.
I've even left plates unwrapped figuring they would have to go bad, and so far nothing. Finally I took a test plate and mashed my finger into the agar just so I could see what would grow. Whole lotta nothing fortunately or unfortunately.
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Culliganman360
Stranger


Registered: 06/05/19
Posts: 854
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I have a lot of plates where growth is touching the edge
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naturalistic123



Registered: 02/18/18
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That looks like tissue plates, the discoloration relating to the tissue itself perhaps. It all looks good to me, i'd use any of that plate except for the swirly 12oclock, but thats just my personal OCD.
Hope all works out!
-------------------- Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Now that I think about it condensation might have something todo with it?
Most of the no pour plates I’ve seen contam have lots of condensation.
-------------------- Tek's I use LAGM2020
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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I dont think it's a matter of the plate itself contaminating, I think its 2 things:
It's easier to see/transfer and distinguish myc types before they hit the edge, after they hit the edge they get intermingled
If contams were on the agar surface you'd be able to see it, but you cant see contams on the edge because there is no agar/food for them to grow on. These could be picked up by the myc and end up on your next plate/grain. even if the contams never germinate/bloom on the overgrown plate, they might once they hit food
It's more of a best practices for transfers/a2g/LC/LI thing than an "it will contam the plate if it hits the edge" thing. It's not that there are contams on the edge, it's that there could be and you wouldnt know until you put it to grain/transfer/etc
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (03/05/20 07:56 PM)
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CatsLoveHouseMusic
SpeakerFreaker



Registered: 11/03/18
Posts: 1,085
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Re: Colorblind guy tries agar [Re: c10h12n2o]
#26519560 - 03/05/20 08:38 PM (3 years, 11 months ago) |
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There is an app I saw someone use on Reddit where they changed the color somehow so they could see contams. I think it ended up having the sub look black, and contams looked some kind of blue. I may be wrong about the colors but check it out. Idk much about that stuff bud sorry
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Maylink
One with Gratitude



Registered: 08/29/19
Posts: 321
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Re: Colorblind guy tries agar [Re: Satanic]
#26519819 - 03/06/20 12:02 AM (3 years, 11 months ago) |
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Side note and just an idea: have you considered adding food coloring to your agar?
Many do it and might be helpful to see contrast and growth of mycelium or mold. And maybe over time be able distinguish by shapes and shades?
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Mr Solo Dolo


Registered: 02/24/19
Posts: 243
Loc: U.S
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Re: Colorblind guy tries agar [Re: Maylink]
#26519832 - 03/06/20 12:24 AM (3 years, 11 months ago) |
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Looks okay.. definitely do more transfers before grain tho..
Contams only happen if contams are present.. I believe the main point of not letting myc hitting the edges is the culture loses vigor (definitely correct me if I'm wrong).. I've put many cultures to grain that hit the side without problems as well.. only a problem if you're trying to keep very vigorous growth in cultures in general
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  LIFE'S A TRIP..  
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Raccoon
Newb


Registered: 04/08/19
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Quote:
c10h12n2o said: It's not that there are contams on the edge, it's that there could be and you wouldnt know until you put it to grain/transfer/etc
Awesome point, C10. I never thought about it this way, and this explanation requires no speculation. We can speculate on whether the edge is more likely to have a contam then the rest of the plate or whether the myc growth loses vigor, but in the end agar is used to KNOW if there is a contam or not, and you don't know if the edge has one. You could still pull the contam from the edge during agar removal to inoculate grain, but that seems less likely than having myc engulfing the edge of the dish pulling potential contams.
Edited by Raccoon (03/06/20 01:33 AM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Quote:
Raccoon said:
Quote:
c10h12n2o said: It's not that there are contams on the edge, it's that there could be and you wouldnt know until you put it to grain/transfer/etc
Awesome point, C10. I never thought about it this way, and this explanation requires no speculation. We can speculate on whether the edge is more likely to have a contam then the rest of the plate or whether the myc growth loses vigor, but in the end agar is used to KNOW if there is a contam or not, and you don't know if the edge has one. You could still pull the contam from the edge during agar removal to inoculate grain, but that seems less likely than having myc engulfing the edge of the dish pulling potential contams.
Exactly 
Quote:
ZombieVibes said: Looks okay.. definitely do more transfers before grain tho..
Contams only happen if contams are present.. I believe the main point of not letting myc hitting the edges is the culture loses vigor (definitely correct me if I'm wrong).. I've put many cultures to grain that hit the side without problems as well.. only a problem if you're trying to keep very vigorous growth in cultures in general

No that's not it. It's the reasons I explained above. The idea of cultures "losing vigor" with too many transfers is greatly exaggerated , and the idea of losing vigor in the same plate is absurd
Think about it: myc expands probably 1000 times more in a grain jar than it does on an agar plate, and probably another 10 times more on a bulk sub. Does myc "lose vigor" when it hits grain? Ofc not.. the exact opposite usually. Is a clone culture less vigorous than it was on the agar its grain spawn came from? Ofc not. Maybe after 100 grows, but not in these short time frames for sure
A plate hitting the edge does not mean it WILL contam, only that there is a possibilty of picking up unseen contams (which you'd see if they were on the agar itself, kinda defeating one of the main advantages to using agar)
And you lose the visibility advantage for spotting different strain groupings and different types of myc once they intermingle at the edge.
For example:

Look closely at the red lines I drew. These separate 2 different types of myc growing on top of each other. If I transfer now, I can get the better looking one on it's own on the next plate, but if I let it grow to the edge that would be impossible since there would be no part of the plate where they had not mingled.
I know it's hard to see, but does that make sense?
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Mr Solo Dolo


Registered: 02/24/19
Posts: 243
Loc: U.S
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Re: Colorblind guy tries agar [Re: c10h12n2o]
#26524188 - 03/08/20 04:04 PM (3 years, 11 months ago) |
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That makes sense I always thought about the slants and how little surface area there is and how it most likely be touching the sides after some time.. Definitely puts my mind at ease

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Colorblind-Myc



Registered: 12/16/19
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Watching.... A thread for me.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Quote:
Mr Solo Dolo said: That makes sense I always thought about the slants and how little surface area there is and how it most likely be touching the sides after some time.. Definitely puts my mind at ease
 
Right, but you wouldnt be going from slant straight to grain, typically you'd grow it out on another plate first. Also, based on the way you use slants, you wouldn't need visibility to be looking for multiple types of myc growing over each other and trying to transfer one or the other (like you would on a plate)
So both of the reasons to transfer before it hits the edge dont really apply with slants
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Mr Solo Dolo


Registered: 02/24/19
Posts: 243
Loc: U.S
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Re: Colorblind guy tries agar [Re: c10h12n2o]
#26524413 - 03/08/20 06:46 PM (3 years, 11 months ago) |
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Yeah that's true .. definitely need to try some out.. lost all my old plates from my last grows that were in a fridge for a few months RIP
Learn something new everyday around here
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  LIFE'S A TRIP..  
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Nichrome
I'm a torso!



Registered: 12/17/18
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"Better looking" 
I love all the myc patterns.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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