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OfflineDr3
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Is this mold in a plate? * 1
    #26516938 - 03/04/20 05:35 PM (3 years, 11 months ago)

So I got this agar plate, thai variety. Apart from the white spots, I would like to know if the wispy growth on the borders is mold. I inoc'd a jar and it went badly, as you can see in the pic. From what I've been reading on the forums it could be penicillin, aspergillus posibly?

Thanks a lot for the help.



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Invisiblewildernessjunkie
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Re: Is this mold in a plate? [Re: Dr3] * 1
    #26517038 - 03/04/20 06:11 PM (3 years, 11 months ago)

The pics of that plate look fine to me. That jar is funky though.


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Offlinebootedboy
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Re: Is this mold in a plate? [Re: wildernessjunkie]
    #26517097 - 03/04/20 06:38 PM (3 years, 11 months ago)

Quote:

wildernessjunkie said:
The pics of that plate look fine to me. That jar is funky though.



Agreed


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OfflineEclipse3130
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Re: Is this mold in a plate? [Re: bootedboy]
    #26517112 - 03/04/20 06:44 PM (3 years, 11 months ago)

The plate could have mold, but most likely not, probably your technique of inoculation


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OfflineSockadin
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Re: Is this mold in a plate? [Re: Eclipse3130]
    #26517149 - 03/04/20 06:55 PM (3 years, 11 months ago)

Jar is way overhydrated. Did it get PC'd long enough? Did your filter fail?

Hard to tell on the plate because it looks clean. But mold can hide in cultures. More likey bad grain prep.


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OfflineDr3
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Re: Is this mold in a plate? [Re: Sockadin]
    #26517872 - 03/05/20 02:32 AM (3 years, 11 months ago)

Thanks for your answers. Jar is definitely bad, yep.I've been having trouble with overhydration during PC cycle because I use these pp5 containers and water seems to get in. Since I can't seem to be able to fix it maybe I will just pre-hydrate the oats for a shorter time, next time.

If you can see it, there are tiny white dots in the plate. These are pins then I suppose?

Thanks for the feedback!


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OfflineLogicaL ChaosM
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Re: Is this mold in a plate? [Re: wildernessjunkie]
    #26517904 - 03/05/20 03:23 AM (3 years, 11 months ago)

Quote:

wildernessjunkie said:
The pics of that plate look fine to me. That jar is funky though.




Exactly my thoughts. Plates are good, jar is junk.


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InvisibleInfiniteDreams
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Re: Is this mold in a plate? [Re: LogicaL Chaos] * 1
    #26518059 - 03/05/20 06:49 AM (3 years, 11 months ago)

Jar looks way way too wet.  What is your technique there?


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Offlinemr. whothehell
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Re: Is this mold in a plate? [Re: InfiniteDreams]
    #26518064 - 03/05/20 06:53 AM (3 years, 11 months ago)

the dishes looks good but probably there are some hidden contam in them, the jar are ready for the trash bag.


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Offlinefootpath
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Re: Is this mold in a plate? [Re: Dr3]
    #26518080 - 03/05/20 07:06 AM (3 years, 11 months ago)

Being wet doesn't cause contamination. Why it's wet might be the cause, however.
It was introduced somewhere. Either before sterilizing and you didn't sterilize properly. Or after sterilizing and your equipment or technique was improper... which could easily be from the original culture.
The plate is way past the point of being able to obviously identify any contaminant cultures, but it does look fine... for whatever that's worth... which is not much.
Those tiny white dots are primordium. Which will become pins. That still doesn't really give an insight as to whether or not there's a contaminant present.


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InvisibleInfiniteDreams
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Re: Is this mold in a plate? [Re: footpath] * 1
    #26518161 - 03/05/20 08:04 AM (3 years, 11 months ago)

Being too wet can allow a contamination to take hold where it wouldn't otherwise be able to.


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Offlinefootpath
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Re: Is this mold in a plate? [Re: InfiniteDreams]
    #26518202 - 03/05/20 08:41 AM (3 years, 11 months ago)

So the implication is it's all good to transfer around contaminants if your spawn substrate is prepped well enough to inhibit their growth? What happens when you transfer it to the more moist bulk substrate?
Obviously warm and wet conditions give the opportunity for more microbes to proliferate, but they have to get there in the first place. That's what OP's concern should be for right now.


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InvisibleInfiniteDreams
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Re: Is this mold in a plate? [Re: footpath]
    #26518211 - 03/05/20 08:49 AM (3 years, 11 months ago)

There never was such an implication, I don't know where you came up with that.

OPs concern should be being able to read and follow directions which didn't happen if he ended up with jars that wet.

I'd argue OP may have a bit of a grasp on sterile technique to produce those agar plates, but who knows?  Maybe he swabbed spores while cleaning out the chicken coop.


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OfflineShroom_Realm
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Re: Is this mold in a plate? [Re: Dr3]
    #26518232 - 03/05/20 09:11 AM (3 years, 11 months ago)

I’m no expert but some experienced growers told me don’t let the plate get colonized to the edges or it could possibly introduce contam somehow. Like I said I’m no expert. I’ll be trying agar to WBS again soon. My last ones stalled at 80% for some reason


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InvisibleInfiniteDreams
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Re: Is this mold in a plate? [Re: Shroom_Realm]
    #26518245 - 03/05/20 09:22 AM (3 years, 11 months ago)

The thought there is that contamination that finds its way in will be at the outside.  With wrapped plates though, it seems like a very small chance.  It is also an excuse to use your plates sooner.


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Offlinefootpath
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Re: Is this mold in a plate? [Re: InfiniteDreams]
    #26518322 - 03/05/20 10:18 AM (3 years, 11 months ago)

Excuse me, inference.

By saying,
Being too wet can allow a contamination to take hold where it wouldn't otherwise be able to.
In response to,
Being wet doesn't cause contamination. Why it's wet might be the cause, however. It was introduced somewhere.
You can easily infer that,
it's all good to transfer around contaminants if your spawn substrate is prepped well enough to inhibit their growth
Because you're saying that a contaminant in dry conditions won't take hold, you leave open the inference that drier conditions can eliminate the ability for contamination to contaminate, or 'take hold'.
It's misleading at best.

Also the thought with not taking from the edge of a cell culturing plate is that there is no substrate on the sidewall for a present microbe to be able to grow, so you leave open the opportunity to transfer something that you cannot see...because it's inhibited and, once in conditions that allow the opportunity for it to grow (like wet, nutritious things), it will then contaminate.


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InvisibleInfiniteDreams
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Re: Is this mold in a plate? [Re: footpath]
    #26518348 - 03/05/20 10:33 AM (3 years, 11 months ago)

:rolleyes:

Yes, I am misleading someone by suggesting they follow TC teks and aim for drier grains...

From RR:

Quote:

Nibin gave the best answer, but I should add that sterilization is never 100% complete with grains.  In addition, filters on bags and/or jars are never 100% effective.  A 99.99% filter will still let that .001% of bacteria in the air get through, and if you sleep in the room, have dogs and cats, run a vacuum cleaner, etc., there's a LOT of bacteria in the air.

With dry grains, you give the mycelium the advantage and it can colonize before the less favored competitors.  Mycelium won't colonize the wet grains at the bottom of the jars, which leaves the spot perfectly suited for bacteria.
RR




And what Nibin said:

Quote:


c) Many contaminants struggle to thrive on drier surfaces, especially if they are surface dwellers like bacteria without the penetrating abilities of fungi.

d) Over wet grains make it harder for mycelium to colonize the grain, giving any bacteria present more time to grow and get stronger than the mycelium.

e) Very over wet grains will even prevent mycelium from growing at all, starving it from air. In this situation any anaerobic bacteria which would struggle to thrive in loose, well aired grain will thrive.




But yes, I did not mean to imply, infer, or otherwise suggest that contamination spontaneously arises from the ether.  And again, I will point out that we are presented 3 pictures originally.  2 very nice looking plates that suggest a grasp of sterile technique and 1 pic of a jar that looks like it is being stored in a swimming pool.

And certainly I am also misinformed about the lurking super bugs hiding on plastic.


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Offlinefootpath
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Re: Is this mold in a plate? [Re: InfiniteDreams]
    #26518389 - 03/05/20 10:56 AM (3 years, 11 months ago)

You can follow whatever instruction you please, it doesn't make wet conditions cause contamination. It has to be otherwise introduced.

It's okay to be misinformed, it's just best to not go spreading it around.


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InvisibleInfiniteDreams
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Re: Is this mold in a plate? [Re: footpath]
    #26518408 - 03/05/20 11:08 AM (3 years, 11 months ago)

I never said that, you came up with that and falsely attributed it to me.

Good luck with your contamination issues footpath.  If you continue to run into trouble, I highly suggest reading through the teks published by TCs and following them carefully and closely.  It has worked very well for me so far.


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Offlinefootpath
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Re: Is this mold in a plate? [Re: InfiniteDreams]
    #26518427 - 03/05/20 11:20 AM (3 years, 11 months ago)

I never said you said that. I said that it could be inferred from your statement in response to mine about wet conditions.

Never thought to look at a Shroomery TC publication for my lessons into the physical properties of microbiological locomotion, I'll have to take a look at some of those. :cheers:


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