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Offlinepoisoned
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Registered: 04/17/13
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Re: Dried gils on agar [Re: cli_hlt]
    #26570402 - 04/01/20 05:21 AM (3 years, 9 months ago)

Make sure to pull in the air bubble while holding a needle over the burner!


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OfflineNewShroomBunny
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Re: Dried gils on agar [Re: cli_hlt]
    #26571872 - 04/01/20 07:21 PM (3 years, 9 months ago)

Thanks for the tips!

I got rid of it asap.

I actually did use a SAB. I'm hoping the bacteria came from the agar and not the syringe. Agar was sitting in my fridge for a few months. I'll keep an update on the my only decent looking agar dish haha.


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Offlinecli_hlt
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Re: Dried gils on agar [Re: NewShroomBunny]
    #26572672 - 04/02/20 06:37 AM (3 years, 9 months ago)

Sometimes you can get enough mycelium amongst loads of contamination that you can transfer away some. Don't get discouraged so quickly. :smile: That plate did look pretty grim btw. :frown:


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OfflineNewShroomBunny
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Re: Dried gils on agar [Re: poisoned]
    #26629679 - 04/26/20 11:59 AM (3 years, 8 months ago)

Do you mind explaining this?

I have a feeling your comment has to do with the popping sounds while the needle was over the burner and likely why I ended up with a lot of contam. Is there any way to prevent liquid from staying in the needle?

Any instructions on technique is very much appreciated.


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Re: Dried gils on agar [Re: cli_hlt]
    #26629691 - 04/26/20 12:03 PM (3 years, 8 months ago)

Thanks for the help! I ended up getting rid of almost all of them as yeast took over. I wish I transferred the healthy myc sooner instead of waiting it out. Still learning.


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Offlinepoisoned
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Re: Dried gils on agar [Re: NewShroomBunny]
    #26629765 - 04/26/20 12:34 PM (3 years, 8 months ago)

So, there's two techniques. 1st, you gotta shake those spores. It's good to have an air bubble in the syringe and to do that, you get your needle red hot and draw in some air over the flame.

2nd, you need to sterilize it before inoculating. you bring it to red hot, then push on the plunger until you get a nice droplet, which means the needle has cooled enough for inoculation.


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OfflineNewShroomBunny
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Re: Dried gils on agar [Re: poisoned]
    #26631556 - 04/27/20 07:14 AM (3 years, 8 months ago)

Right, I did all of that. I was innoc-ing multiple bags and read that I should burn the needle for each bag. My issue was after the first bag, liquid remained in the needle and would start popping when under the needle. Don't know if you have any advice on that? If I'm using a SAB, is it necessary to burn the needle for each inoculation?


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