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Invisiblethemushroombloke
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Registered: 08/20/11
Posts: 448
Loc: Australia
Re: Developing a specific phenotype [Re: B Traven]
    #27932907 - 09/03/22 10:12 PM (1 year, 4 months ago)

Quote:

B Traven said:
I think the way mushrooms grow in the wild is substantially different from what we're doing.

You've got dried-out cowpies that will occasionally revive after a storm, reviving a colony that then goes on to produce fruits, which drop spores on that and similar colonies, meaning many generations are intermingling their mycelium. Also, spore dispersal means that genetic material from a very wide area is intermingling on a regular basis.

So then if you're trying to narrow down the genetics, the first thing that's going to affect the effort is how far you are from a wild-type. I know I have no idea when it comes to the spores I'm working with. It's basically "X generations from wild plus the number of generations I personally have pushed through."

Sporulation resets the genetic clock, sure, but it can't recover genetics that have been removed from circulation via selective agar transfers and cloning. Unlike the situation in an open field, your cultures are never intermingling with new myc fromm some wind-blown spores. Even if you're only narrowing the gene pool by, say, 1% every time you do a clone-print-grow cycle, you'll eventually start to see the effects.

I really want to step up to do some conscious breeding at some point, but for now I'm just amusing myself with sequential cloning. I've got a few twice-and-thrice-cloned plates. I think it'd be fun to get up to like 4 or 5 sequential clones, then grow them out side by side.




I think this guy from Fresh from the Farm Fungi explains it well in some of his video's. Here's one that's a brief overview.

and here's a link to his awesome breeding project that took ages and he grew  out all these different sectors on the agar and choose colonies that cross bred, extremely cool! here's the link for the playlist enjoy!!!!!


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Invisiblethemushroombloke
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Registered: 08/20/11
Posts: 448
Loc: Australia
Re: Developing a specific phenotype [Re: A.k.a]
    #27932912 - 09/03/22 10:14 PM (1 year, 4 months ago)

Quote:

A.k.a said:
Daaaaamn this is old!

Look at all the TC tags now :awesomenod:




:zombie5: brains!!! it's alive!!


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