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OfflineBackspacey
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Registered: 03/04/20
Posts: 8
Last seen: 3 years, 5 months
Sclerotia Cloning - Atlantis & Mexicana
    #26515970 - 03/04/20 07:04 AM (3 years, 10 months ago)

Hello All,

I am from The Netherlands where sclerotia is legal (excuse me for my English).

This is my first post on this forum and also my first attempt doing any kind of mushroom/agar work. I've read a lot on this and other forums already, quite fun so far.

I bought some truffles (Atlantis & Mexicana) a couple of months ago but didn't had the time to do anything with them (eating). So I finally made the first step into mushroom cultivation.

I already had some pre-mix MEA laying around. The outer package told me I had to use 50grams of MEA on 1 liter of water PCed for 15 mins @ 15 psi. From what I've read here most use 40 per liter.

I tried two different methods in order get some sclerotia tissue on a petri dish.
1: Breaking the sclerotia in halve and cut a little piece out of there.
  After 2 weeks there were no signs of growth.
2: Putting a whole sclerotia in 90% iso, drying it in front of the flowhood, then cut in halve (ofc flame sterilized scalpel) and put on the agarplate. Signs of growth appeared after around 1.5 - 2 weeks.

No mold or bacteria. BUT now it looks like the center is turning brownish/yelowish. Is this bacteria / old mycelium or old sclerotia dying?



PS had to do some rewrap with polytape as the parafilm had gotten old.


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Offlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,937
Loc: 7b
Last seen: 1 day, 1 hour
Trusted Cultivator
Re: Sclerotia Cloning - Atlantis & Mexicana [Re: Backspacey]
    #26516065 - 03/04/20 08:00 AM (3 years, 10 months ago)

brown growth is normal for Atl or mexicana.
your plate looks good
Usually growth only takes about 48 hours to be visible. Two weeks is crazy.
You have a flowhood and you never tried to do agar work before?
Welcome to the shroomey :hi:


Edited by bw86 (03/04/20 08:01 AM)


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OfflineBackspacey
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Registered: 03/04/20
Posts: 8
Last seen: 3 years, 5 months
Re: Sclerotia Cloning - Atlantis & Mexicana [Re: bw86]
    #26516158 - 03/04/20 09:20 AM (3 years, 10 months ago)

Thanks for the answer I was hoping for.

Indeed the first time doing this, majority of the plates ended up in the trash hehe.

A friend of mine gave me his flowhood among some other stuf a while ago as he had no more use for it. I watched him doing a few things here and there, now I know why there were so many Azurescens growing in the woods.


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