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Grumio

Registered: 10/18/19
Posts: 126
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Cloning to agar: pin vs mature fruit?
#26513947 - 03/03/20 04:50 AM (3 years, 10 months ago) |
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What is the general rule?
I've heard people say cloning a pin is good as it's not as "dirty" as a mature fruit.
Then in my head I think I could clone a pin and it turns out to only mature into a small fruit.
Therefore I think cloning mature fruits would be better but can someone else enlighten me?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cloning to agar: pin vs mature fruit? [Re: Grumio]
#26513960 - 03/03/20 05:04 AM (3 years, 10 months ago) |
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I don't clone pins i put them on agar in their entirety. I clone mature fruits because they're clone-able
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cloning to agar: pin vs mature fruit? [Re: bodhisatta]
#26513963 - 03/03/20 05:06 AM (3 years, 10 months ago) |
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Grumio

Registered: 10/18/19
Posts: 126
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Re: Cloning to agar: pin vs mature fruit? [Re: bodhisatta]
#26514024 - 03/03/20 06:14 AM (3 years, 10 months ago) |
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Quote:
bodhisatta said: I don't clone pins i put them on agar in their entirety. I clone mature fruits because they're clone-able
Thanks for correcting me, I indeed meant putting the whole pin onto agar vs a piece of mature fruit flesh from inside the stem.
Do you prefer to put pins to agar or clone a mature fruit?
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A.k.a
Stranger



Registered: 10/27/19
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Re: Cloning to agar: pin vs mature fruit? [Re: Grumio]
#26514211 - 03/03/20 08:53 AM (3 years, 10 months ago) |
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From what I’ve read the reason to clone pins is they grow really quickly.
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LAGM2020     
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shevanel
Gone til November


Registered: 01/27/08
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Re: Cloning to agar: pin vs mature fruit? [Re: A.k.a]
#26514221 - 03/03/20 08:59 AM (3 years, 10 months ago) |
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watching
---- i need to get your agar recipe, that looks sweet.
Quote:
bodhisatta said:

Edited by shevanel (03/03/20 09:01 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cloning to agar: pin vs mature fruit? [Re: shevanel]
#26514276 - 03/03/20 09:34 AM (3 years, 10 months ago) |
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Its in the agar tek. Just malt agar
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Mycobum7
Stranger


Registered: 01/28/19 
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Re: Cloning to agar: pin vs mature fruit? [Re: Grumio]
#26514283 - 03/03/20 09:36 AM (3 years, 10 months ago) |
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im new but i was under the impression part of the point of cloning would be to select a fruit with preferred traits? in that case, wouldnt choosing a pin be a weird way to select a clone?
Also, how are they "cleaner" arent we to take tissue from inside the fruit?
-------------------- "This isn't my first rodeo ya know.......its like my third."
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The Mycologist
Explorer

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Re: Cloning to agar: pin vs mature fruit? [Re: Mycobum7]
#26514296 - 03/03/20 09:44 AM (3 years, 10 months ago) |
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A pin on agar shows you that it at least likes to fruit.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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feldman114
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Registered: 09/06/19
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Re: Cloning to agar: pin vs mature fruit? [Re: Mycobum7]
#26514299 - 03/03/20 09:45 AM (3 years, 10 months ago) |
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I love when my MS agar pins - free, sterile clone without any work. But I’d never put a pin from a mono on agar. Maybe I’m wrong but it seems like a guarantee of nasties in the culture.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cloning to agar: pin vs mature fruit? [Re: feldman114]
#26514306 - 03/03/20 09:49 AM (3 years, 10 months ago) |
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You can open a colonized jar dump it out on the carpet. Pick one grain up with your bare fingers. Put it onto agar. And easily get a clean culture.
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feldman114
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Re: Cloning to agar: pin vs mature fruit? [Re: bodhisatta]
#26514311 - 03/03/20 09:52 AM (3 years, 10 months ago) |
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Whaaaa??
I know nothing, Jon Snow.
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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Re: Cloning to agar: pin vs mature fruit? [Re: bodhisatta]
#26514388 - 03/03/20 10:35 AM (3 years, 10 months ago) |
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since i had no idea wtf i was doing yet i used premix mea from a vendor.
ill revisit the tek as i am getting down to 0g left
(mine comes out peach colored i like how yours are clear, looks neat)
Edited by shevanel (03/03/20 10:36 AM)
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Shr00mEater
Strange

Registered: 10/17/18
Posts: 985
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Re: Cloning to agar: pin vs mature fruit? [Re: feldman114]
#26514530 - 03/03/20 12:31 PM (3 years, 10 months ago) |
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Quote:
bodhisatta said: You can open a colonized jar dump it out on the carpet. Pick one grain up with your bare fingers. Put it onto agar. And easily get a clean culture.
Quote:
feldman114 said:

Whaaaa??
I know nothing, Jon Snow.
LOL! I was thinking a grain>floor>agar tek would be in order.
I assume the keyword is colonized. After the mycelium colonizes the grains, it is fairly hardy. Even if a hitchhiker was picked up from the floor, and put on agar, it will be very hard for the contaminant to get established. The myc is already in control of the major resources and has developed its own defenses.
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