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OfflineThe Mycologist
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Registered: 05/06/16
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Re: Liquid Culture Question [Re: cna8]
    #26518044 - 03/05/20 08:34 AM (1 year, 2 months ago)

Spores to LC wont work.


You have to use agar first. Get it clean then put it in lc.


Get into agar. One drop per plate unlimited genetics to use.


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Offlinemr. whothehell
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Re: Liquid Culture Question [Re: Grim11311]
    #26518055 - 03/05/20 08:47 AM (1 year, 2 months ago)

you put too much grain in your pop corn jar, fill it  just 2/3. your LC looks good, BUT it's impossible to tell if it's good.


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OfflineGrim11311
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Re: Liquid Culture Question [Re: mr. whothehell]
    #26518074 - 03/05/20 09:01 AM (1 year, 2 months ago)

Is that why only 1 shows signs of growth and the other 4 dont.... because it is too full of grains?


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Offlinecna8
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Re: Liquid Culture Question [Re: Grim11311]
    #26518266 - 03/05/20 11:38 AM (1 year, 2 months ago)

Hey bro.
Too full just means it might stall or be really hard to shake.
It can take 3 weeks to germinate so at this stage it doesn't mean anything.


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OfflineGrim11311
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Re: Liquid Culture Question [Re: cna8]
    #26518338 - 03/05/20 12:27 PM (1 year, 2 months ago)

Thanks for your help bro.... just saw signs of life in another jar... so 2 of the 5 have signs of life


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Offlinemr. whothehell
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Re: Liquid Culture Question [Re: Grim11311]
    #26519821 - 03/06/20 02:07 AM (1 year, 2 months ago)

give them some more time, no if they are too full they will be diffcult too shake


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OfflineOvoidhunter
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Re: Liquid Culture Question [Re: mr. whothehell]
    #26520058 - 03/06/20 08:28 AM (1 year, 2 months ago)

Don't let people get you down. Spores to LC can work an has in the past. You can get lucky and it's not some impossible feat.. I'm rooting for u dude.


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OfflineThe Mycologist
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Re: Liquid Culture Question [Re: Ovoidhunter] * 1
    #26520064 - 03/06/20 08:34 AM (1 year, 2 months ago)

Planning to get lucky isnt a good plan :mad2:


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineOvoidhunter
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Re: Liquid Culture Question [Re: The Mycologist]
    #26520069 - 03/06/20 08:41 AM (1 year, 2 months ago)

Bro calm down. Just because he didn't know and he did it the unorthodox way your gonna be flustered. Why not let him do it for science.. I know it's not the most foolproof but just chill dude. Why not just tell him to open it up and take a shit in it.. Dang

We learn things in life by trial and error. Even if it doesn't work he's still going to learn something. He's gonna learn not to do it that way again. If it doesn't work. Which it still could.

:whattefuck2:


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OfflineThe Mycologist
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Re: Liquid Culture Question [Re: Ovoidhunter]
    #26520088 - 03/06/20 08:58 AM (1 year, 2 months ago)

I let people do what they want.

But he asked for help. So I am trying to help him grow shrooms.

I guess you are trying to help him play with chance.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineOvoidhunter
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Re: Liquid Culture Question [Re: The Mycologist]
    #26520095 - 03/06/20 09:07 AM (1 year, 2 months ago)

I'm just not going to discourage him and tell him it's not going to work. OP knows by now he should have went to agar first. Everyone's already told him.

Also he said he had to wait forever for supplies to get shipped in and if it doesn't work he's screwed. So I'm going to give him words of encouragement. Instead of saying black and white up and down it won't work.


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OfflineThe Mycologist
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Re: Liquid Culture Question [Re: Ovoidhunter]
    #26520168 - 03/06/20 10:02 AM (1 year, 2 months ago)

He would be better off doing pf tek then.

I honestly think it is a waste of grain to use that lc.

Im not trying to be mean, Im just saying what I would do if I wanted shrooms and didnt want to waste my money.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineOvoidhunter
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Re: Liquid Culture Question [Re: The Mycologist] * 1
    #26520173 - 03/06/20 10:10 AM (1 year, 2 months ago)

I feel you on that. PF tek is a better way to start.  Sorry for being defensive of him so much. However I'd let him use the LC just for shits and giggles. If it doesn't work he'll know better next time and we're all here to listen and learn. OP would be wise to listen to your advice and get into agar. At the same time tho he should figure stuff out for himself and experience failure.

No hard feelings to you mycologist.


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OfflineGrim11311
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Re: Liquid Culture Question [Re: Ovoidhunter]
    #26520263 - 03/06/20 11:20 AM (1 year, 2 months ago)

Hi guys... read your posts... so what i understand from it is there is no way the LC will work... not even the slightest of chance even though it looks good and there is no visible contamination? I am preping some WBS and popcorn to soak for 12h+ and wil make a syringe tomorrow to inoculate the grains.... maybe i will get lucky though.
Uploaded some pics of how the LC looks today



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OfflineOvoidhunter
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Re: Liquid Culture Question [Re: Grim11311]
    #26520271 - 03/06/20 11:29 AM (1 year, 2 months ago)

You do have hope. Those look fine to me but you gotta test it to know. I was just saying earlier that you can go spores to LC and people have done it successfully. Of course that's no reason to not use agar to isolate your culture first.  Percentage of success would no doubt be higher.

Still tho wishing you luck OP. It definitely still can work.


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Offlinecna8
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Re: Liquid Culture Question [Re: Ovoidhunter]
    #26520334 - 03/06/20 11:59 AM (1 year, 2 months ago)

Bro it looks good like he said so I would test it not ditch it.
I used to go spores to liquid culture.
I also used to go spores to grain.
I used to get a higher rate of failures then.
About a 50:50.
Modern teqniques tend to be easier and or more successful that is why we use agar now for example.
testing it is what you gotta do to know if there's no films and no discoloration and the gowth isn't disorganized.
Try get the agar like I said but in the mean time, test it and hope, the gods of mushrooms smile appon you.
If your print is as clean as I make and your luck is like mine, you should have 2 or 3 successful inoculations.
Don't be disappointed if you don't though.

Good Luck


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OfflineThe Mycologist
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Re: Liquid Culture Question [Re: Grim11311]
    #26520437 - 03/06/20 01:12 PM (1 year, 2 months ago)

If you dont care about maybe wasting grain then go for it.

I would just top case the jars after they are colonized, I think even if its clean enough to use, spawning to bulk would probably be too risky.

Ovoid: No worries bro.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Offlinecna8
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Re: Liquid Culture Question [Re: The Mycologist]
    #26520503 - 03/06/20 02:02 PM (1 year, 2 months ago)

Yea bro he had a real hard time getting the syringe and only has 2ml left so it's definitely worth it for him.
Even if he fruits directly off the grain in the jar to make his own prints so he has some safety.
Anyways...

Good Luck


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OfflineGrim11311
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Re: Liquid Culture Question [Re: cna8]
    #26520535 - 03/06/20 02:20 PM (1 year, 2 months ago)

Yes cna8... that is what i thought as well. Even if the LC fail to produce anything i could do g2g transfer when jar is completely colonized and spawn to bulk from there... Even if i could just get a few shrooms (wouldn't mind for a lot though) to make my own spore prints,spore syringes or LC using a piece of shroom it would be a huge accomplishment for me... ordered some patri dishes already... still looking for a place in this shitty 3rd world country of mine for some agar... if i cant find i will probably have to order online as well.


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Offlinecna8
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Re: Liquid Culture Question [Re: Grim11311]
    #26520553 - 03/06/20 02:34 PM (1 year, 2 months ago)

Look for "agar agar"
It tends to be easier to find.
I agree with him about casing the jar.
The less steps the less chance of contamination.
Once you have some extra spores then go hard.
I recommend grain to grain at a 1:10 ratio, if your set on doin it straight away.
I don't use liquid culture anymore just grain to grain.
Anyways...

Good Luck


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Shop: Bridgetown Botanicals Bridgetown Botanicals   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Amazon ½ Pint Jars, Agar, Wild Bird Seed

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