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OfflineGrim11311
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Liquid Culture Question
    #26513814 - 03/03/20 02:15 AM (1 year, 2 months ago)

Hi guys... need a little bit of your help. I made my first liquid culture... i uploaded some pics which i took today... this is on day 8 of the process. Just wanted to know if it looks good to you and how much longer i should keep it going. Thanks for your time

It is golden teacher
Honey and maple syrup and filtered water mixture



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Offlinecna8
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Re: Liquid Culture Question [Re: Grim11311]
    #26513883 - 03/03/20 04:25 AM (1 year, 2 months ago)

Good-aye Bro.

those photo's are really blurry.
Does it look like organized growth or just chaos?
is it all white? as in no discolored parts?
Did you start with agar?
Can you take better photos with a plain background?
And try get good lighting please.

Here is a how it should look thread
https://www.shroomery.org/forums/showflat.php/Number/17231150
Search for liquid in your browser and you should be able to skip to the part you want.
Notice how hard it is too see what you are looking at?
However on the agar it is really easy to tell the difference.

I highly recommend testing on agar to see if its clean.
That's the best way in my opinion.
Then you can guarantee your growing what you want.
Because a clean looking Liquid Culture can be contaminated.

Good Luck bro


Edited by cna8 (03/03/20 04:32 AM)


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OfflineGrim11311
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Re: Liquid Culture Question [Re: cna8]
    #26513927 - 03/03/20 06:03 AM (1 year, 2 months ago)

Hi will try to take better pictures tonight... no just had to do it from a spore syringe i bought. I cant seem to find agar where i live
It looks white when i shine a torch in there and it is in a roundish shape at the bottom of the jar. When i swirl it it breaks up into pieces


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OfflineOvoidhunter
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Re: Liquid Culture Question [Re: Grim11311]
    #26513931 - 03/03/20 06:08 AM (1 year, 2 months ago)

No way to know for sure with LC but to test it. Put it to some grains. Also they say you shouldn't put spores directly to LC but I don't like to discourage folks. It looks good to me. :mushroom2:

Edit: what is the thing in the bottom there?


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Edited by Ovoidhunter (03/03/20 06:10 AM)


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OfflineGrim11311
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Re: Liquid Culture Question [Re: Ovoidhunter]
    #26513966 - 03/03/20 07:09 AM (1 year, 2 months ago)

It is 3 small marbles i put in to break up the mycelium


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OfflineOvoidhunter
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Re: Liquid Culture Question [Re: Grim11311]
    #26513980 - 03/03/20 07:23 AM (1 year, 2 months ago)

Wow, that's some innovative stuff grim. I'm exited to see how this turns out I just nocced some LC the same way.


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OfflineGrim11311
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Re: Liquid Culture Question [Re: Ovoidhunter]
    #26514021 - 03/03/20 08:13 AM (1 year, 2 months ago)

Thanks Ovoidhunter... really hope this works because i struggled my ass off to get the syringe and after a month of waiting for it i did 5 jars popcorn.. on day 9 only one looks like it started to grow. The rest i nocced in the liquid culture and have only about 2cc left in the syringe


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OfflineGrim11311
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Re: Liquid Culture Question [Re: Grim11311]
    #26514023 - 03/03/20 08:14 AM (1 year, 2 months ago)

Hope yours turn out well


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OfflineGrim11311
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Re: Liquid Culture Question [Re: Grim11311]
    #26514244 - 03/03/20 11:15 AM (1 year, 2 months ago)

Here are the pics i took tonight... and of the popcorn jars i inoculated the same time as the LC. One shows signs of growth but the other 4 shows nothing



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Offlinecna8
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Re: Liquid Culture Question [Re: Grim11311]
    #26515614 - 03/04/20 01:01 AM (1 year, 2 months ago)

from what i can see it looks all right.
Although without testing that doesn't mean much.
order some agar online if needed.
It is well worth it.
even if you have to order agar agar separately just add it to some grain boil water then PC it(i prefer rye water).
In the mean time do what Ovoidhunter said.

try not to break it up with a marble until your going to use it.
Then you can see how its growing when you get the lighting right.

Those jars you inoculated at the same time.
Not all will be successful i had a 50:50 ratio when i started like that.
Spores can take up to three weeks to germinate.

Wishing you the best bro.
Good Luck


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OfflineGrim11311
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Re: Liquid Culture Question [Re: cna8]
    #26515633 - 03/04/20 01:18 AM (1 year, 2 months ago)

Thank you for the reply and help so far cna8... will try to get some agar. So i should just leave the LC and not swirl it everyday?


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Offlinecna8
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Re: Liquid Culture Question [Re: Grim11311]
    #26515646 - 03/04/20 01:38 AM (1 year, 2 months ago)

Yea bro leave it.
It is hard to see in photos but you should be able to see how organized the growth is if you can get light to hit it right.
If you mess it up it makes it harder to tell from other more disorganized members of the fungi family.
Good Luck


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OfflineGrim11311
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Re: Liquid Culture Question [Re: cna8]
    #26516212 - 03/04/20 11:59 AM (1 year, 2 months ago)

This is what it looks like today... did not stir... just took it out of the closet... tried to get the best lighting



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OfflineThe Mycologist
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Re: Liquid Culture Question [Re: Grim11311]
    #26516220 - 03/04/20 12:05 PM (1 year, 2 months ago)

How did you inoculate the lc?


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Offlinecna8
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Re: Liquid Culture Question [Re: The Mycologist]
    #26516273 - 03/04/20 12:46 PM (1 year, 2 months ago)

Hey Mycologist
he used a spore syringe.
Hes only got 2ml left now.
Im at the stage where i think hes gotta test it.
Hope you can help him more,
Peace


Edited by cna8 (03/04/20 12:48 PM)


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OfflineGrim11311
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Re: Liquid Culture Question [Re: cna8]
    #26516307 - 03/04/20 01:17 PM (1 year, 2 months ago)

Hi Mycologist... used a spore syringe I bought and inoculated 5 popcorn jars... only 1 showing signs of growth... also did the LC and have 2 cc left in the syringe... really hope either the jars or the LC works or both... really had a hard time getting my hands on the syringe


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OfflineGrim11311
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Re: Liquid Culture Question [Re: Grim11311]
    #26517688 - 03/05/20 01:02 AM (1 year, 2 months ago)

Quote:

Grim11311 said:
This is what it looks like today... did not stir... just took it out of the closet... tried to get the best lighting








From these pictures.... can i suck up some of the culture and test it on some popcorn or wbs jars already or must i still wait a few days


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Offlinecna8
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Re: Liquid Culture Question [Re: Grim11311]
    #26517743 - 03/05/20 01:51 AM (1 year, 2 months ago)

Yea bro go ahead and test it.
This is not scientific i don't even know if this is right but in my experience,
If you see any film on the side of the jar or the surface throw it out its bacteria,
Next time you do one don't shake it.
Molds usually show them self after about a week at room temp.
However because you shook it it will be really hard to tell from white molds.
I know things can probably germinate and show way slower but in my experience that is enough time.
If this is bad advice someone correct me i may have just gotten lucky when iv'e done it.

Good Luck bro


Edited by cna8 (03/05/20 01:53 AM)


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OfflineGrim11311
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Re: Liquid Culture Question [Re: cna8]
    #26517752 - 03/05/20 02:04 AM (1 year, 2 months ago)

Thanks cna8... will do some jars this weekend... kinda hard to prep all the things after work... will keep you posted on my progress


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Offlinecna8
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Re: Liquid Culture Question [Re: Grim11311]
    #26517770 - 03/05/20 02:20 AM (1 year, 2 months ago)

All good bro.
Sounds like a good plan
Check again in the weekend for any discoloration etc.
See if the growth flows (it may not, it was shaken so don't ditch it yet if it doesn't)
Look for bacterial films.
If it all looks lovely test your marble shacker and your culture.
Wishing you luck.


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Shop: Bridgetown Botanicals Bridgetown Botanicals   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Amazon ½ Pint Jars, Agar, Rye Grain, Wild Bird Seed

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