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ToxicMush



Registered: 03/02/20
Posts: 7
Last seen: 2 years, 9 months
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First time, GT, G2G ready? Mycelium piss?
#26513805 - 03/02/20 11:57 PM (3 years, 10 months ago) |
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Whatsup Mush Fam? So as most people, I’ve been lurking the shroomery for awhile, (years on and off) before deciding to finally make an account. Now that I’ve obtained a syringe of GT’s and successfully sterilized my spawn(rye), I inoculated a few jars and an LC, in the Beginning of February, and it seems to be going well.... one jar I gave about 2 cc while the others 1.. the 1 I started the 3rd last month (2cc) seems to have a tan yellowish liquid coming out of some areas... I’ve seen pictures and read that this is harmless and just called “mycelium piss” or something like that... I just want some more recent, advice from someone that’s experienced the same, and I feel like my jar is about fully colonized but I’m honestly not sure. Anyone have experience with the Golden teachers? All advice is welcome! Will have pics up soon on this thread, but have them in my pictures ATM - Thanks It won’t let me post any pictures or videos for some reason.. it’s says there’s a picture of one of my jars in my Gallery, is there a reason for this?? And I took it upon myself to shake the jar up because it honestly looks like cobweb mold after I’ve been searching the forum... but what would be the reasons I would have cobweb if I did everything obnoxiously sterile? One flaw I know I made is my Qt jars are definitely more filled than they should be... they have about 2 - 3 inches of air space. I’m sorry for the read, but I’ve put a lot in, and want success!
Edited by ToxicMush (03/03/20 04:10 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First time, GT, G2G ready? Mycelium piss? [Re: ToxicMush] 1
#26513948 - 03/03/20 04:53 AM (3 years, 10 months ago) |
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You shouldn't be getting metabolites in grain jars growing cubensis. More spores = more contamination. Only takes a drop of spore solution to have enough spores. Spore prints/syringes are not axenic so use as little as needed to inoculate. Preferably start with agar
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Cootermonkey
Seeker/lover of knowledge


Registered: 01/30/18
Posts: 272
Loc: Texas
Last seen: 1 year, 4 months
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Re: First time, GT, G2G ready? Mycelium piss? [Re: ToxicMush]
#26513958 - 03/03/20 05:04 AM (3 years, 10 months ago) |
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Damn the big guy beat me to it haha, already typed this chit up so im still submitting itxD
Well pictures will definetly help out alot more, as fot the "myc piss" thats likely metabolites which means theres a contamination, still might get fruits and you might not depends on the contam To me it sounds like you did all that with the syringe-which is possible to work but youll likely lose a bunch to contamination.
Much better to go buy from an asian market or order online some agar agar powder and make some up, can even use the water from boiling your grains as the media. That will help insure and assure you to get a clean culture going from a few transfers after germination.
All while stretching the spore syringe significantly since you only need a drop or two to get growth, Then once youve got a clean culture going(doesnt have to be an isolate just clean) you can try Lc because more than likely the Lc's you made up are contaminated. To finish- I wouldnt G2g, if they grow out well you can still spawn your qt jars to a few shoeboxes and maybe at least get some fruits which you could then clone your favorite (looking,biggest,whatever really)fruit to the agar and help reduce your time to next harvest dramatically as well as have more faith in a nice clean growing culture that could make you some canopies!
-------------------- MyLAGM2020Growlog
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ToxicMush



Registered: 03/02/20
Posts: 7
Last seen: 2 years, 9 months
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Re: First time, GT, G2G ready? Mycelium piss? [Re: bodhisatta]
#26514540 - 03/03/20 12:40 PM (3 years, 10 months ago) |
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Okay thanks... even if there’s only a very little of the metabolite? Does this mean I should toss this jar? Should I not grain to grain this one? I think I might just play around with it to learn more about contamination as well, thanks for the advice
-------------------- “I wonder what would happen if there were a United Organization of Organisms (UOO, pronounced “uh-oh”), where each species gets one vote. Would we be voted off the planet? The answer is pretty clear.” -Paul Stamets “Nature alone is antique, and the oldest art a Mushroom” - Thomas Carlyle
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ToxicMush



Registered: 03/02/20
Posts: 7
Last seen: 2 years, 9 months
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Re: First time, GT, G2G ready? Mycelium piss? [Re: Cootermonkey]
#26514551 - 03/03/20 12:47 PM (3 years, 10 months ago) |
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Okay I see... so more than likely the one jar is contam the others are going well, looks like clean growth to me, what would you do with the metabolite jar? Experiment or toss? And as for Agar, I thought if you didn’t use a flow hood your chances for success were minimal.. I can make SAB but flow hoods gonna have to wait lol and what potential, contam would I be able to fruit through? Cobweb? Thanks again , both of your responses more than likely just saved me a bunch of RYE and a bunch of TIME lmfao
-------------------- “I wonder what would happen if there were a United Organization of Organisms (UOO, pronounced “uh-oh”), where each species gets one vote. Would we be voted off the planet? The answer is pretty clear.” -Paul Stamets “Nature alone is antique, and the oldest art a Mushroom” - Thomas Carlyle
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SynKyd
ctrl-alt-delite



Registered: 09/27/13
Posts: 1,554
Loc: ૐ
Last seen: 2 years, 9 months
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Re: First time, GT, G2G ready? Mycelium piss? [Re: ToxicMush]
#26514786 - 03/03/20 03:02 PM (3 years, 10 months ago) |
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Post some pics, impossible to assess this without them.
-------------------- New inclusive poop emojis from Apple!
   
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ToxicMush



Registered: 03/02/20
Posts: 7
Last seen: 2 years, 9 months
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Re: First time, GT, G2G ready? Mycelium piss? [Re: SynKyd]
#26514814 - 03/03/20 03:17 PM (3 years, 10 months ago) |
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Log in to view attachment
I’ve been trying to get a picture up, but it’s denying my picture and saying I need a video.. the pictures are in my files on the shroomery.. idk why it’s not letting me post it
-------------------- “I wonder what would happen if there were a United Organization of Organisms (UOO, pronounced “uh-oh”), where each species gets one vote. Would we be voted off the planet? The answer is pretty clear.” -Paul Stamets “Nature alone is antique, and the oldest art a Mushroom” - Thomas Carlyle
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Cootermonkey
Seeker/lover of knowledge


Registered: 01/30/18
Posts: 272
Loc: Texas
Last seen: 1 year, 4 months
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Re: First time, GT, G2G ready? Mycelium piss? [Re: ToxicMush] 1
#26515468 - 03/03/20 08:43 PM (3 years, 10 months ago) |
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SAB works perfectly fine with sterile procedures and techniques, if it didnt most people on here probably wouldnt have very much success(including me) haha. bodhisatta up there even has some videos posted to show you exactly how easy things can be. I myself would not grain to grain a contaminated jar period haha, as far as experimentation, could always just do a small shoebox use the 1 with some coir. Then it wont be such a waste as making a bunch of jars that would still be contaminated, i havent looked at the pics cause i dont click links but just use your best judgement, take notes thats what will make it scientific, a way to learn and improve. Otherwise its like youre just pullin your pecker the wrong way your whole life xD
-------------------- MyLAGM2020Growlog
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
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Re: First time, GT, G2G ready? Mycelium piss? [Re: ToxicMush]
#26515544 - 03/03/20 09:53 PM (3 years, 10 months ago) |
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Quote:
ToxicMush said: I’ve been trying to get a picture up, but it’s denying my picture and saying I need a video.. the pictures are in my files on the shroomery.. idk why it’s not letting me post it
For uploading pictures, you want this button.

Or this link

Not this one.

Sounds like that may be the issue.
as for the grain, I definitely would not recommend using anything you think could be contaminated for grain to grain. As far agar, and SAB is all I and many others have used to great success. Some excellent teks on here to get the basics from. MSS to grain can be done but certainly not the best way to go. A little less than 50% success rate at best. If all you have is MSS and don't want to do agar, I would stick to BRF\PF Tek until then simply because you will have much better success rate. I say all of this as a fellow noob that went through the same process in the not so distant past.
Edited by Randalf the Grey (03/03/20 09:59 PM)
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